Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GTQ55_RS16865 Genome accession   NZ_CP047491
Coordinates   4002602..4003636 (+) Length   344 a.a.
NCBI ID   WP_161859776.1    Uniprot ID   A0A6P1TCG6
Organism   Microbulbifer hydrolyticus strain IRE-31     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3997602..4008636
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GTQ55_RS16845 (GTQ55_16855) - 3998186..4000153 (-) 1968 WP_161859773.1 dynamin family protein -
  GTQ55_RS16850 (GTQ55_16860) - 4000354..4000959 (-) 606 WP_161859774.1 YggT family protein -
  GTQ55_RS16855 (GTQ55_16865) proC 4000971..4001798 (-) 828 WP_237567739.1 pyrroline-5-carboxylate reductase -
  GTQ55_RS16860 (GTQ55_16870) - 4001825..4002520 (-) 696 WP_161859775.1 YggS family pyridoxal phosphate-dependent enzyme -
  GTQ55_RS16865 (GTQ55_16875) pilT 4002602..4003636 (+) 1035 WP_161859776.1 type IV pilus twitching motility protein PilT Machinery gene
  GTQ55_RS16870 (GTQ55_16880) pilU 4003638..4004786 (+) 1149 WP_161859777.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GTQ55_RS16875 (GTQ55_16885) - 4004886..4006577 (+) 1692 WP_161859778.1 amidohydrolase -
  GTQ55_RS16880 (GTQ55_16890) - 4006603..4007367 (-) 765 WP_202620642.1 LytR/AlgR family response regulator transcription factor -
  GTQ55_RS16885 (GTQ55_16895) - 4007364..4008566 (-) 1203 WP_161859779.1 sensor histidine kinase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38398.03 Da        Isoelectric Point: 6.5788

>NTDB_id=413791 GTQ55_RS16865 WP_161859776.1 4002602..4003636(+) (pilT) [Microbulbifer hydrolyticus strain IRE-31]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDVRRINLPPMEHKQVHGLIYEIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNHNRGAGAVFRTIPSKVLTMEDLGMGQVFKQISDTPRGLVLVTGPTGSGKSTSLAAMIDYINDNKYEHILTVED
PIEFVHESKKCLVNQREVHRDTHGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAQEKAMVRSMLSESLQAVISQTLLKRNGGGRVAAHEIMRGTPAIRNLIREDKIAQMYSAIQTGASVGMQTMDQCL
QDLVEKRIISREVAREKAKMPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=413791 GTQ55_RS16865 WP_161859776.1 4002602..4003636(+) (pilT) [Microbulbifer hydrolyticus strain IRE-31]
ATGGACATTACAGAACTCCTCGCCTTCAGCGCCAAGCAAAACGCCTCGGACTTGCACCTCTCGGCAGGTCTGCCCCCGAT
GATCCGGGTAGACGGCGACGTGCGCCGCATCAACCTGCCTCCGATGGAGCACAAGCAGGTTCACGGCCTGATCTACGAGA
TCATGAACGACAAGCAGCGCAAGGACTACGAAGAGTTCCTCGAGACCGACTTCTCCTTCGAAGTCCCGGGCGTGGCGCGC
TTTCGTGTGAACGCGTTCAACCACAACCGCGGCGCCGGCGCCGTATTCCGGACCATTCCCTCCAAGGTACTCACCATGGA
AGACCTGGGGATGGGCCAGGTGTTCAAGCAGATCTCCGACACCCCCCGCGGCCTGGTTCTGGTGACCGGCCCTACCGGTT
CCGGTAAGTCCACCAGCCTCGCGGCAATGATCGACTACATCAACGACAACAAGTACGAGCACATCCTCACGGTCGAAGAC
CCGATCGAATTCGTGCACGAATCCAAGAAGTGCCTGGTCAACCAGCGGGAAGTCCACCGCGACACCCACGGCTTCTCCGA
AGCACTGCGCTCGGCACTGCGTGAAGACCCGGACATCATCCTCGTGGGTGAGATGCGGGACCTCGAGACCATCCGTCTGG
CGCTGACCGCCGCGGAAACCGGTCACCTGGTATTCGGTACCCTGCACACCACCTCCGCGGCCAAAACCATCGACCGTGTT
GTCGACGTGTTCCCGGCGCAGGAAAAAGCCATGGTGCGCTCCATGTTGTCGGAATCCCTGCAGGCAGTAATCTCGCAGAC
CCTGCTCAAGCGCAACGGCGGTGGCCGTGTGGCCGCCCACGAAATCATGCGCGGCACCCCGGCCATCCGGAACCTCATCC
GCGAAGACAAGATCGCGCAGATGTACTCCGCCATCCAGACCGGGGCCAGTGTGGGCATGCAGACCATGGACCAATGCCTG
CAGGACCTGGTGGAAAAACGCATCATCAGCCGAGAAGTAGCGCGCGAAAAAGCGAAAATGCCGGAGAATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6P1TCG6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

89.244

100

0.892
  pilT Pseudomonas stutzeri DSM 10701

87.791

100

0.878
  pilT Acinetobacter nosocomialis M2

83.721

100

0.837
  pilT Acinetobacter baumannii D1279779

83.721

100

0.837
  pilT Acinetobacter baumannii strain A118

83.721

100

0.837
  pilT Acinetobacter baylyi ADP1

80.233

100

0.802
  pilT Legionella pneumophila strain Lp02

75.74

98.256

0.744
  pilT Legionella pneumophila strain ERS1305867

75.74

98.256

0.744
  pilT Neisseria meningitidis 8013

67.826

100

0.68
  pilT Neisseria gonorrhoeae MS11

67.246

100

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.647

98.837

0.669
  pilT Vibrio cholerae strain A1552

67.647

98.837

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.517
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Vibrio cholerae strain A1552

40.118

98.547

0.395
  pilU Acinetobacter baylyi ADP1

39.017

100

0.392

Multiple sequence alignment