Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RTA_RS02395 Genome accession   NC_015677
Coordinates   485708..486751 (+) Length   347 a.a.
NCBI ID   WP_013899782.1    Uniprot ID   F5XVH3
Organism   Ramlibacter tataouinensis TTB310     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 480708..491751
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RTA_RS02360 (Rta_04660) - 480883..481362 (+) 480 WP_041675946.1 VOC family protein -
  RTA_RS02365 (Rta_04670) - 481487..482641 (+) 1155 WP_193384809.1 methyltransferase -
  RTA_RS02370 (Rta_04680) - 482663..483502 (-) 840 WP_041674978.1 PA4780 family RIO1-like protein kinase -
  RTA_RS02375 (Rta_04690) - 483595..483993 (-) 399 Protein_499 ankyrin repeat domain-containing protein -
  RTA_RS02380 (Rta_04700) - 484350..484604 (+) 255 Protein_500 RNA recognition motif domain-containing protein -
  RTA_RS02385 (Rta_04710) infA 484637..484903 (+) 267 WP_013899780.1 translation initiation factor IF-1 -
  RTA_RS02390 (Rta_04720) - 484982..485674 (-) 693 WP_013899781.1 YggS family pyridoxal phosphate-dependent enzyme -
  RTA_RS02395 (Rta_04730) pilT 485708..486751 (+) 1044 WP_013899782.1 type IV pilus twitching motility protein PilT Machinery gene
  RTA_RS02400 (Rta_04740) pilU 486767..487903 (+) 1137 WP_013899783.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RTA_RS02405 (Rta_04750) - 488102..489004 (+) 903 WP_013899784.1 NAD(P)-dependent oxidoreductase -
  RTA_RS02410 (Rta_04760) - 489141..489767 (+) 627 WP_013899785.1 (2Fe-2S)-binding protein -
  RTA_RS02415 (Rta_04770) - 489811..490788 (+) 978 WP_013899786.1 xanthine dehydrogenase family protein subunit M -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38263.06 Da        Isoelectric Point: 6.7674

>NTDB_id=41342 RTA_RS02395 WP_013899782.1 485708..486751(+) (pilT) [Ramlibacter tataouinensis TTB310]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDALDHKQVHAMVYDIMNDTQRKNYEEFLECDFSFEIDGLAR
FRVNAFNQNRGAAAVFRTIPSKILSLEQLNAPKIFGELALKPRGMVLVTGPTGSGKSTTLAAMVNYLNETEYGHILTVED
PIEFVHESKKCLVNQREVGPMTLSFAAALKSALREDPDCILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPSEEKEMVRAMLSESLQAVISQTLCKTKDGNGRVAAHEIMLGTSAIRNLIREAKVAQMYSAIQTGNSFGMQTLDQN
LADLVKRNMISPAEARSKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=41342 RTA_RS02395 WP_013899782.1 485708..486751(+) (pilT) [Ramlibacter tataouinensis TTB310]
GTGGATATTACCCAGCTGCTGGCATTCAGCGTGAAGAACAAGGCGTCCGACCTGCACCTGTCCGCCGGCCTGCCCCCCAT
GATCCGCGTGCACGGCGACGTGCGCCGCATCAACGTCGACGCGCTGGACCACAAGCAGGTGCACGCCATGGTGTACGACA
TCATGAACGACACCCAGCGCAAGAACTACGAGGAGTTCCTGGAGTGCGACTTCTCGTTCGAGATCGACGGCCTGGCGCGC
TTCCGCGTCAACGCCTTCAACCAGAACCGCGGCGCGGCCGCCGTATTCCGGACCATCCCGTCCAAGATCCTCTCGCTCGA
GCAGCTCAACGCGCCCAAGATCTTCGGCGAGCTGGCGCTCAAGCCGCGCGGCATGGTGCTGGTGACCGGCCCCACCGGCT
CGGGCAAGTCGACCACGCTGGCCGCGATGGTGAACTACCTGAACGAGACCGAGTACGGCCACATCCTGACGGTGGAGGAC
CCGATCGAGTTCGTGCACGAGTCCAAGAAATGCCTGGTGAACCAGCGCGAGGTCGGGCCGATGACGCTGTCGTTCGCCGC
GGCGCTCAAGTCGGCGCTGCGCGAGGACCCGGACTGCATCCTGGTGGGCGAGATGCGCGACCTGGAGACCATCCGCCTGG
CGATGACCGCCGCCGAGACTGGCCACCTGGTGTTCGGCACGCTGCACACCTCCAGTGCCGCCAAGACCATCGACCGGATC
ATCGATGTGTTCCCGTCGGAGGAAAAGGAGATGGTGCGCGCCATGCTGTCCGAGTCGCTGCAGGCGGTGATCTCGCAGAC
GCTGTGCAAGACCAAGGACGGCAACGGCCGGGTGGCCGCGCACGAGATCATGCTGGGCACCAGCGCCATCCGCAACCTGA
TCCGCGAGGCCAAGGTGGCGCAGATGTACTCGGCGATACAGACCGGCAACAGCTTCGGCATGCAGACCCTGGACCAGAAC
CTGGCTGACCTGGTCAAGCGCAACATGATCAGCCCGGCCGAAGCCCGCAGCAAGGCCAAGATCCCCGAGAACTTTCCCGG
ATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F5XVH3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.391

99.424

0.769
  pilT Pseudomonas stutzeri DSM 10701

76.522

99.424

0.761
  pilT Acinetobacter nosocomialis M2

76.232

99.424

0.758
  pilT Acinetobacter baumannii D1279779

76.232

99.424

0.758
  pilT Acinetobacter baumannii strain A118

76.232

99.424

0.758
  pilT Acinetobacter baylyi ADP1

74.203

99.424

0.738
  pilT Legionella pneumophila strain Lp02

74.041

97.695

0.723
  pilT Legionella pneumophila strain ERS1305867

74.041

97.695

0.723
  pilT Neisseria gonorrhoeae MS11

68.406

99.424

0.68
  pilT Neisseria meningitidis 8013

68.406

99.424

0.68
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.862

98.271

0.657
  pilT Vibrio cholerae strain A1552

66.862

98.271

0.657
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

99.424

0.493
  pilU Vibrio cholerae strain A1552

43.284

96.542

0.418
  pilU Acinetobacter baylyi ADP1

41.04

99.712

0.409
  pilU Pseudomonas stutzeri DSM 10701

41.493

96.542

0.401

Multiple sequence alignment