Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RTA_RS02400 Genome accession   NC_015677
Coordinates   486767..487903 (+) Length   378 a.a.
NCBI ID   WP_013899783.1    Uniprot ID   F5XVH4
Organism   Ramlibacter tataouinensis TTB310     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 481767..492903
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RTA_RS02370 (Rta_04680) - 482663..483502 (-) 840 WP_041674978.1 PA4780 family RIO1-like protein kinase -
  RTA_RS02375 (Rta_04690) - 483595..483993 (-) 399 Protein_499 ankyrin repeat domain-containing protein -
  RTA_RS02380 (Rta_04700) - 484350..484604 (+) 255 Protein_500 RNA recognition motif domain-containing protein -
  RTA_RS02385 (Rta_04710) infA 484637..484903 (+) 267 WP_013899780.1 translation initiation factor IF-1 -
  RTA_RS02390 (Rta_04720) - 484982..485674 (-) 693 WP_013899781.1 YggS family pyridoxal phosphate-dependent enzyme -
  RTA_RS02395 (Rta_04730) pilT 485708..486751 (+) 1044 WP_013899782.1 type IV pilus twitching motility protein PilT Machinery gene
  RTA_RS02400 (Rta_04740) pilU 486767..487903 (+) 1137 WP_013899783.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RTA_RS02405 (Rta_04750) - 488102..489004 (+) 903 WP_013899784.1 NAD(P)-dependent oxidoreductase -
  RTA_RS02410 (Rta_04760) - 489141..489767 (+) 627 WP_013899785.1 (2Fe-2S)-binding protein -
  RTA_RS02415 (Rta_04770) - 489811..490788 (+) 978 WP_013899786.1 xanthine dehydrogenase family protein subunit M -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41713.05 Da        Isoelectric Point: 6.9078

>NTDB_id=41343 RTA_RS02400 WP_013899783.1 486767..487903(+) (pilU) [Ramlibacter tataouinensis TTB310]
MERDQASKFINDLLKLMVSRNGSDLFITGDFPPAIKVDGKVTKVSPQPLNAGHTLALARSIMNDKQASEFERTKECNFAI
SPGGIGRFRVNAFVQQGKVGMVLRVIPQVLPTIDGLGVPQVLKEVVMAKRGLVVLVGATGSGKSTTLAAMIDWRNEQSQG
HIITIEDPVEFVHPHKNCVVTQREVGLDTDSWEAALKNTLRQAPDVILMGEVRDRETMEHAIAFAETGHLCMCTLHANSA
NQALDRIINFFPEERRTQLLMDLSLNLKGMVSQRLVPKQDGKGRAAAVEIMLNTPLISDLIFKGEVSEIKEIMKKSRNLG
MQTFDQALFDAYEGNVISYEDALRNADSVNDLRLQIKLNSQRAKTQDLSAGTEHLAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=41343 RTA_RS02400 WP_013899783.1 486767..487903(+) (pilU) [Ramlibacter tataouinensis TTB310]
ATGGAACGCGACCAGGCCAGCAAATTCATCAACGACCTGCTCAAGCTGATGGTCAGCCGCAACGGCAGCGACCTGTTCAT
CACCGGCGACTTCCCGCCCGCCATCAAGGTGGACGGCAAGGTCACCAAGGTCTCGCCGCAGCCGCTGAACGCCGGCCACA
CGCTGGCGCTGGCCCGCTCGATCATGAACGACAAGCAGGCCTCGGAGTTCGAGCGCACCAAGGAGTGCAACTTCGCGATC
TCGCCGGGGGGCATCGGCCGCTTCCGCGTCAACGCCTTCGTGCAGCAGGGCAAGGTCGGCATGGTGCTGCGGGTGATCCC
GCAGGTGCTGCCCACCATCGACGGCCTGGGCGTGCCGCAGGTGCTCAAGGAGGTGGTGATGGCCAAGCGCGGCCTGGTGG
TGCTGGTGGGCGCCACCGGCTCGGGCAAGTCGACCACGCTGGCGGCCATGATCGACTGGCGCAACGAGCAGAGCCAGGGC
CACATCATCACCATCGAGGATCCGGTGGAATTCGTGCACCCGCACAAGAACTGCGTGGTGACGCAGCGCGAGGTCGGCCT
GGACACCGACAGCTGGGAGGCGGCGCTGAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATGGGCGAGGTGCGCG
ACCGCGAGACCATGGAGCACGCGATCGCCTTCGCCGAGACCGGCCACCTGTGCATGTGCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGGATCATCAACTTCTTCCCCGAGGAGCGCCGCACCCAGCTGCTGATGGACCTGTCGCTCAACCT
CAAGGGCATGGTGTCGCAGCGCCTGGTGCCCAAGCAGGACGGCAAGGGCCGCGCGGCGGCGGTGGAGATCATGCTCAACA
CCCCGCTGATCTCCGACCTGATCTTCAAGGGCGAGGTCTCCGAGATCAAGGAGATCATGAAGAAGAGCCGCAACCTGGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGATGCCTACGAGGGCAACGTCATCAGCTACGAGGACGCGCTGCGCAACGCCGA
CTCGGTCAACGACCTGCGGCTGCAGATCAAGCTGAACAGCCAGCGGGCCAAGACCCAGGACCTGTCGGCGGGCACCGAGC
ACCTGGCCATCGTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F5XVH4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.897

92.328

0.59
  pilU Acinetobacter baylyi ADP1

57.382

94.974

0.545
  pilU Vibrio cholerae strain A1552

54.023

92.063

0.497
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.104

89.153

0.402
  pilT Pseudomonas aeruginosa PAK

44.51

89.153

0.397
  pilT Legionella pneumophila strain ERS1305867

43.844

88.095

0.386
  pilT Legionella pneumophila strain Lp02

43.844

88.095

0.386
  pilT Pseudomonas stutzeri DSM 10701

43.027

89.153

0.384
  pilT Acinetobacter nosocomialis M2

42.943

88.095

0.378
  pilT Acinetobacter baumannii D1279779

42.943

88.095

0.378
  pilT Acinetobacter baumannii strain A118

42.943

88.095

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.533

83.862

0.365
  pilT Vibrio cholerae strain A1552

43.533

83.862

0.365

Multiple sequence alignment