Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GSF12_RS08460 Genome accession   NZ_CP047226
Coordinates   1870390..1871439 (+) Length   349 a.a.
NCBI ID   WP_095355904.1    Uniprot ID   -
Organism   Moraxella osloensis strain YV1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1865390..1876439
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GSF12_RS08440 (GSF12_08440) truD 1865665..1866753 (+) 1089 WP_159375123.1 tRNA pseudouridine(13) synthase TruD -
  GSF12_RS08445 (GSF12_08445) - 1866762..1868111 (-) 1350 WP_159375124.1 sensor domain-containing diguanylate cyclase -
  GSF12_RS08450 (GSF12_08450) - 1868448..1868972 (-) 525 WP_076776333.1 C40 family peptidase -
  GSF12_RS08455 (GSF12_08455) - 1869398..1870123 (-) 726 WP_201450383.1 YggS family pyridoxal phosphate-dependent enzyme -
  GSF12_RS08460 (GSF12_08460) pilT 1870390..1871439 (+) 1050 WP_095355904.1 type IV pilus twitching motility protein PilT Machinery gene
  GSF12_RS08465 (GSF12_08465) pilU 1871529..1872680 (+) 1152 WP_159375125.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GSF12_RS08470 (GSF12_08470) - 1872752..1873288 (-) 537 WP_159375126.1 GNAT family N-acetyltransferase -
  GSF12_RS08475 (GSF12_08475) ribF 1873414..1874412 (+) 999 WP_159375127.1 riboflavin biosynthesis protein RibF -
  GSF12_RS08480 (GSF12_08480) - 1874419..1874955 (-) 537 WP_159375128.1 hypothetical protein -
  GSF12_RS08485 (GSF12_08485) - 1875198..1876328 (+) 1131 WP_159375129.1 transposase -

Sequence

Protein


Download         Length: 349 a.a.        Molecular weight: 38919.63 Da        Isoelectric Point: 6.7822

>NTDB_id=411609 GSF12_RS08460 WP_095355904.1 1870390..1871439(+) (pilT) [Moraxella osloensis strain YV1]
MAKELSIDDLLRFAFKNKASDLHISAGLPPMIRVDGEITKINMPPLDHQKVHSLIYDIMNDKQRADYEEFFETDFSFEIP
NVARFRVNAFNQNRGAGAVFRTIPSEVLTMEQLGMGPIFEKISNYKRGVVLVTGPTGSGKSTTLAAMINYINETRKEHIL
TIEDPIEFVHQSKKSLINQREVHRDTLSFDAALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKT
IDRVVDVFPAAEKDMIRAMLSESLQAVISQSLLKRQTGGRVAAHEIMIGTPAIRNLIRENKVAQMYSAIQTSAGDGMTTL
DQSLRELVNKGVISKESARQYAKQPESFM

Nucleotide


Download         Length: 1050 bp        

>NTDB_id=411609 GSF12_RS08460 WP_095355904.1 1870390..1871439(+) (pilT) [Moraxella osloensis strain YV1]
ATGGCAAAGGAATTATCGATTGACGATTTGTTGCGTTTTGCATTCAAAAATAAAGCCTCTGACTTACACATTTCAGCAGG
CTTACCGCCGATGATTCGGGTAGATGGTGAAATCACCAAAATCAATATGCCGCCGCTTGATCACCAAAAAGTGCATAGTT
TGATTTATGACATCATGAACGACAAACAACGTGCCGATTATGAAGAGTTTTTTGAAACCGACTTTTCGTTTGAAATTCCT
AATGTGGCGCGTTTTCGTGTCAATGCCTTTAACCAAAACCGGGGCGCAGGTGCGGTATTTCGTACCATTCCGTCTGAGGT
CTTGACGATGGAACAGCTGGGTATGGGACCGATTTTTGAGAAAATCTCTAATTACAAACGCGGCGTGGTACTGGTCACAG
GTCCAACGGGTTCAGGTAAGTCAACAACCCTTGCAGCGATGATTAACTATATCAATGAAACCCGTAAAGAACATATCTTA
ACCATCGAAGACCCGATTGAATTTGTGCACCAATCAAAAAAATCCCTCATCAACCAACGGGAAGTCCACCGTGATACCTT
AAGCTTTGACGCCGCACTGCGCTCTGCCCTACGTGAAGACCCCGATGTTATTCTTGTCGGTGAGATGCGTGACCTTGAGA
CCATCCGCTTGGCATTGACGGCTGCTGAAACAGGGCACTTGGTATTTGGGACATTGCATACCTCATCTGCTGCCAAAACC
ATTGACCGTGTGGTCGATGTGTTCCCCGCGGCTGAAAAAGACATGATTCGTGCGATGTTATCAGAATCGCTACAAGCAGT
TATCTCGCAATCGCTACTCAAACGCCAGACAGGTGGTCGTGTCGCCGCGCATGAAATCATGATTGGTACGCCTGCGATTC
GTAACTTAATTCGTGAAAACAAAGTCGCCCAAATGTACTCTGCCATCCAAACCAGTGCGGGCGATGGTATGACCACACTC
GATCAAAGCCTTCGCGAACTGGTCAATAAAGGCGTGATTAGCAAAGAATCGGCGCGCCAATATGCCAAACAACCGGAAAG
CTTTATGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

80.29

98.854

0.794
  pilT Acinetobacter baumannii strain A118

80.29

98.854

0.794
  pilT Acinetobacter baumannii D1279779

80.29

98.854

0.794
  pilT Pseudomonas aeruginosa PAK

77.326

98.567

0.762
  pilT Pseudomonas stutzeri DSM 10701

77.035

98.567

0.759
  pilT Acinetobacter baylyi ADP1

77.035

98.567

0.759
  pilT Legionella pneumophila strain Lp02

73.256

98.567

0.722
  pilT Legionella pneumophila strain ERS1305867

73.256

98.567

0.722
  pilT Neisseria meningitidis 8013

67.826

98.854

0.67
  pilT Neisseria gonorrhoeae MS11

67.536

98.854

0.668
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.976

95.129

0.656
  pilT Vibrio cholerae strain A1552

68.976

95.129

0.656
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.436

100

0.524
  pilU Acinetobacter baylyi ADP1

41.04

99.14

0.407
  pilU Pseudomonas stutzeri DSM 10701

40.708

97.135

0.395
  pilU Vibrio cholerae strain A1552

40

95.989

0.384

Multiple sequence alignment