Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GSF12_RS08465 Genome accession   NZ_CP047226
Coordinates   1871529..1872680 (+) Length   383 a.a.
NCBI ID   WP_159375125.1    Uniprot ID   A0A6P1KJ10
Organism   Moraxella osloensis strain YV1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1866529..1877680
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GSF12_RS08445 (GSF12_08445) - 1866762..1868111 (-) 1350 WP_159375124.1 sensor domain-containing diguanylate cyclase -
  GSF12_RS08450 (GSF12_08450) - 1868448..1868972 (-) 525 WP_076776333.1 C40 family peptidase -
  GSF12_RS08455 (GSF12_08455) - 1869398..1870123 (-) 726 WP_201450383.1 YggS family pyridoxal phosphate-dependent enzyme -
  GSF12_RS08460 (GSF12_08460) pilT 1870390..1871439 (+) 1050 WP_095355904.1 type IV pilus twitching motility protein PilT Machinery gene
  GSF12_RS08465 (GSF12_08465) pilU 1871529..1872680 (+) 1152 WP_159375125.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GSF12_RS08470 (GSF12_08470) - 1872752..1873288 (-) 537 WP_159375126.1 GNAT family N-acetyltransferase -
  GSF12_RS08475 (GSF12_08475) ribF 1873414..1874412 (+) 999 WP_159375127.1 riboflavin biosynthesis protein RibF -
  GSF12_RS08480 (GSF12_08480) - 1874419..1874955 (-) 537 WP_159375128.1 hypothetical protein -
  GSF12_RS08485 (GSF12_08485) - 1875198..1876328 (+) 1131 WP_159375129.1 transposase -
  GSF12_RS08490 (GSF12_08490) - 1876333..1876929 (-) 597 WP_159375130.1 hypothetical protein -

Sequence

Protein


Download         Length: 383 a.a.        Molecular weight: 42939.25 Da        Isoelectric Point: 6.0546

>NTDB_id=411610 GSF12_RS08465 WP_159375125.1 1871529..1872680(+) (pilU) [Moraxella osloensis strain YV1]
MDFDKLLQLMVQKNGSDLFITADAPPSMKVNGTIMPVTKSALTPEQSMTLVKSVMTPAQIKEFDETLECQFAISDKHQNA
RFRVSAFVQKDCAGMVLRKIETKIPTVEQLNLPPILNEIAMKKQGIVLLVGATGTGKSTTLAAMMGYRNENSRGHIITIE
DPIEYVHEHKGCIITQREVGIDTKSFEAGLKNTLRQAPDVILIGEIRTRETMDYAIQYAETGHLVFATLHANNANQAIDR
IIHFFDAEHHEQILMDLSLNLHAIVAQQLIATPDGKGRRAAIEILLNSQLIGDYIRKGEVHEIKPVMKRSRELGMQTFDQ
ALFDLYENGEITYRDAIKHADSPNDLRLLIKLESKSANKIDENELNFSLQSDDHSAPNEKSMF

Nucleotide


Download         Length: 1152 bp        

>NTDB_id=411610 GSF12_RS08465 WP_159375125.1 1871529..1872680(+) (pilU) [Moraxella osloensis strain YV1]
ATGGATTTTGATAAATTACTGCAGCTAATGGTACAAAAAAACGGCTCTGACCTTTTTATCACCGCTGATGCGCCACCATC
AATGAAAGTCAATGGTACTATCATGCCAGTCACCAAGTCAGCCCTGACTCCAGAGCAATCGATGACGCTGGTCAAAAGCG
TCATGACGCCTGCTCAAATCAAAGAATTTGACGAAACCCTCGAATGCCAGTTTGCCATTAGTGACAAACACCAAAACGCC
CGCTTTCGTGTCTCTGCCTTTGTCCAAAAAGACTGTGCGGGCATGGTGCTACGTAAAATTGAAACCAAAATTCCGACGGT
AGAGCAACTCAATCTACCGCCGATTTTGAATGAAATTGCCATGAAAAAACAGGGTATCGTGCTACTGGTCGGTGCGACGG
GTACGGGTAAATCTACCACGCTGGCGGCTATGATGGGTTATCGCAACGAGAATTCGCGCGGTCATATCATCACTATTGAA
GACCCGATTGAATACGTCCATGAGCACAAAGGCTGTATCATCACCCAACGCGAAGTTGGCATTGATACCAAGTCATTTGA
AGCAGGGTTAAAAAACACCCTGCGTCAAGCGCCTGATGTGATTTTGATTGGCGAGATTCGTACCCGCGAAACCATGGATT
ATGCCATTCAATATGCCGAAACAGGACACTTGGTGTTTGCCACCCTACACGCCAACAATGCTAACCAAGCGATTGACCGT
ATCATTCACTTCTTTGACGCAGAACATCATGAGCAGATTTTGATGGATTTGTCATTGAACCTTCACGCCATTGTAGCGCA
GCAATTGATTGCCACCCCTGATGGCAAAGGTCGCCGCGCTGCGATTGAAATTTTGCTCAATTCGCAACTGATTGGTGATT
ACATCCGTAAAGGGGAAGTCCACGAAATTAAGCCCGTCATGAAACGCTCTCGTGAACTTGGCATGCAAACCTTTGACCAA
GCGTTGTTTGATTTGTATGAAAATGGCGAAATTACTTACCGCGATGCCATCAAACACGCTGACTCACCCAATGATTTACG
CCTACTCATTAAGCTGGAGAGTAAATCAGCCAATAAAATTGATGAAAATGAACTAAATTTTTCATTGCAATCTGATGATC
ATAGCGCACCAAATGAGAAATCCATGTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(31-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6P1KJ10

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.258

92.95

0.634
  pilU Acinetobacter baylyi ADP1

64.56

95.039

0.614
  pilU Vibrio cholerae strain A1552

54.986

91.645

0.504
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.45

91.645

0.389
  pilT Acinetobacter baumannii D1279779

41.983

89.556

0.376
  pilT Acinetobacter nosocomialis M2

41.983

89.556

0.376
  pilT Acinetobacter baumannii strain A118

41.983

89.556

0.376
  pilT Acinetobacter baylyi ADP1

41.399

89.556

0.371
  pilT Legionella pneumophila strain Lp02

41.964

87.728

0.368
  pilT Legionella pneumophila strain ERS1305867

41.964

87.728

0.368
  pilT Vibrio cholerae strain A1552

42.296

86.423

0.366
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.296

86.423

0.366
  pilT Pseudomonas stutzeri DSM 10701

40.173

90.339

0.363
  pilT Pseudomonas aeruginosa PAK

40.173

90.339

0.363

Multiple sequence alignment