Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H5C80_RS08665 Genome accession   NZ_CP059907
Coordinates   1738983..1739963 (+) Length   326 a.a.
NCBI ID   WP_182928217.1    Uniprot ID   -
Organism   Escherichia coli strain 181.1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1733983..1744963
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H5C80_RS08635 (H5C80_08635) - 1734357..1735343 (-) 987 WP_000784004.1 TRAP transporter substrate-binding protein -
  H5C80_RS08640 (H5C80_08640) hemW 1735660..1736796 (-) 1137 WP_000239950.1 radical SAM family heme chaperone HemW -
  H5C80_RS08645 (H5C80_08645) rdgB 1736789..1737382 (-) 594 WP_001174747.1 XTP/dITP diphosphatase -
  H5C80_RS08650 (H5C80_08650) yggU 1737390..1737680 (-) 291 WP_001277194.1 DUF167 family protein YggU -
  H5C80_RS08655 (H5C80_08655) yggT 1737677..1738243 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  H5C80_RS08660 (H5C80_08660) yggS 1738261..1738965 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  H5C80_RS08665 (H5C80_08665) pilT 1738983..1739963 (+) 981 WP_182928217.1 type IV pilus twitching motility protein PilT Machinery gene
  H5C80_RS08670 (H5C80_08670) ruvX 1740137..1740553 (-) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  H5C80_RS08675 (H5C80_08675) yqgE 1740553..1741116 (-) 564 WP_001318029.1 YqgE/AlgH family protein -
  H5C80_RS08680 (H5C80_08680) gshB 1741225..1742175 (-) 951 WP_000593258.1 glutathione synthase -
  H5C80_RS08685 (H5C80_08685) rsmE 1742188..1742919 (-) 732 WP_001300912.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  H5C80_RS08690 (H5C80_08690) endA 1742999..1743706 (-) 708 WP_001305312.1 deoxyribonuclease I -
  H5C80_RS08695 (H5C80_08695) yggI 1743801..1744298 (-) 498 WP_001696137.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35935.14 Da        Isoelectric Point: 5.5923

>NTDB_id=411463 H5C80_RS08665 WP_182928217.1 1738983..1739963(+) (pilT) [Escherichia coli strain 181.1]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPAVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETICLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQER
VKEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=411463 H5C80_RS08665 WP_182928217.1 1738983..1739963(+) (pilT) [Escherichia coli strain 181.1]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGCGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGATTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGCATTTCGCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCAGCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTCGAA
TATCTCTATGCAAGCCAGCGATGTTTGATCCAGCAGCGGGAAATCGGTTTGCATTGTATGACGTTCGCATCGGGATTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCTGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCGGCGCAGGCAGTTGAGCGACTGGTAGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCGGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGTTGTTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGTGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGGAGCGG
GTGAAAGAAGGGCGCTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Acinetobacter baylyi ADP1

46.177

100

0.463
  pilT Acinetobacter nosocomialis M2

46.177

100

0.463
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain ERS1305867

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

39.21

100

0.396
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilB Legionella pneumophila strain ERS1305867

31.414

100

0.368
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362