Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H4J01_RS04205 Genome accession   NZ_CP059840
Coordinates   857638..858618 (+) Length   326 a.a.
NCBI ID   WP_012578995.1    Uniprot ID   B7UHZ7
Organism   Escherichia coli strain E2348/69     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 852638..863618
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H4J01_RS04175 yggM 853243..854250 (+) 1008 WP_000745186.1 DUF1202 family protein -
  H4J01_RS04180 hemW 854315..855451 (-) 1137 WP_000239938.1 radical SAM family heme chaperone HemW -
  H4J01_RS04185 rdgB 855444..856037 (-) 594 WP_001174747.1 XTP/dITP diphosphatase -
  H4J01_RS04190 yggU 856045..856335 (-) 291 WP_001277194.1 DUF167 family protein YggU -
  H4J01_RS04195 yggT 856332..856898 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  H4J01_RS04200 yggS 856916..857620 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  H4J01_RS04205 pilT 857638..858618 (+) 981 WP_012578995.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  H4J01_RS04210 ruvX 858625..859041 (-) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  H4J01_RS04215 yqgE 859041..859604 (-) 564 WP_001339287.1 YqgE/AlgH family protein -
  H4J01_RS04220 gshB 859713..860663 (-) 951 WP_000593260.1 glutathione synthase -
  H4J01_RS04225 rsmE 860676..861407 (-) 732 WP_012578994.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  H4J01_RS04230 endA 861486..862193 (-) 708 WP_000286507.1 deoxyribonuclease I -
  H4J01_RS04235 yggI 862288..862785 (-) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35929.12 Da        Isoelectric Point: 5.5860

>NTDB_id=411284 H4J01_RS04205 WP_012578995.1 857638..858618(+) (pilT) [Escherichia coli strain E2348/69]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=411284 H4J01_RS04205 WP_012578995.1 857638..858618(+) (pilT) [Escherichia coli strain E2348/69]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGCCCGCAGCTCGAACAACTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTCGAA
TATCTCTATACCAGTCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCTGCGTTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTACGTGACAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCGGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGGAGTTTACGGGCAGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACTGGGCAACAAGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7UHZ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

40.694

97.239

0.396
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

37.346

99.387

0.371
  pilB Legionella pneumophila strain ERS1305867

30.89

100

0.362

Multiple sequence alignment