Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GQ674_RS03325 Genome accession   NZ_CP047135
Coordinates   700393..701430 (+) Length   345 a.a.
NCBI ID   WP_070208322.1    Uniprot ID   A0A1E7RM02
Organism   Stenotrophomonas sp. 364     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 695393..706430
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GQ674_RS03300 (GQ674_03300) soxR 696544..696978 (+) 435 WP_201290209.1 redox-sensitive transcriptional activator SoxR -
  GQ674_RS03305 (GQ674_03305) - 697114..697923 (-) 810 WP_328803873.1 short chain dehydrogenase -
  GQ674_RS03310 (GQ674_03310) - 697814..698695 (+) 882 WP_159495943.1 LysR substrate-binding domain-containing protein -
  GQ674_RS03315 (GQ674_03315) proC 698773..699594 (-) 822 WP_159495944.1 pyrroline-5-carboxylate reductase -
  GQ674_RS03320 (GQ674_03320) - 699626..700306 (-) 681 WP_201290210.1 YggS family pyridoxal phosphate-dependent enzyme -
  GQ674_RS03325 (GQ674_03325) pilT 700393..701430 (+) 1038 WP_070208322.1 type IV pilus twitching motility protein PilT Machinery gene
  GQ674_RS03330 (GQ674_03330) pilU 701509..702651 (+) 1143 WP_159495945.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GQ674_RS03335 (GQ674_03335) - 702824..703408 (+) 585 WP_159495946.1 DUF4234 domain-containing protein -
  GQ674_RS03340 (GQ674_03340) - 703546..704529 (+) 984 WP_159495947.1 aldo/keto reductase -
  GQ674_RS03345 (GQ674_03345) - 704606..705427 (-) 822 WP_159495948.1 alpha/beta hydrolase -
  GQ674_RS03350 (GQ674_03350) - 705599..705817 (+) 219 WP_159495949.1 DUF4287 domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38569.36 Da        Isoelectric Point: 6.5797

>NTDB_id=410916 GQ674_RS03325 WP_070208322.1 700393..701430(+) (pilT) [Stenotrophomonas sp. 364]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLAVPPIFRELIDQPQGLILVTGPTGSGKSTTLAAMIDHINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQMGMMTLDQHL
QDLVKRSLITRNQAKEYAKDKRIFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=410916 GQ674_RS03325 WP_070208322.1 700393..701430(+) (pilT) [Stenotrophomonas sp. 364]
ATGGATATCGCCGAACTGTTGGCGTTTTCCGTAAAGAACAAAGCGTCGGATCTTCATCTGTCGGCAGGCCTGCCGCCGAT
GATCCGCGTTGACGGCGATGTCCGTCGCATCAACATCCCGGCCTTGGACCACAAGCAGGTGCATGCGCTTGTGTACGACA
TCATGTCGGACAAGCAGCGCCGCGATTACGAGGAATTCCTCGAAGTCGACTTCTCGTTCGAGATTCCGTCGCTTGCGCGC
TTCCGCGTCAATGCGTTCAATCAGAACCGGGGCGCCGGTGCGGTGTTCCGTACCATTCCCTCGGAAGTGTTGACGCTGGA
AGACCTGGCCGTGCCGCCGATCTTCCGCGAACTGATCGACCAGCCGCAGGGCCTGATCCTGGTCACCGGCCCGACCGGTT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACCACATCAACAAGAACGAATACGGCCACATCCTCACCGTCGAGGAT
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGTGACACGCACGGCTTCAACGA
AGCGCTGCGCTCGGCCCTGCGTGAAGACCCGGACATCATCCTGGTCGGCGAATTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCCGAAACCGGTCACCTGGTGTTCGGCACGCTGCATACCAGTTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCCGCCGGCGAGAAGCCGATGGTGCGCTCGATGCTGTCCGAGTCGCTGCGTGCGGTGATCTCCCAGGC
CCTGCTGAAGAAAGTCGGTGGCGGGCGTACCGCCGCGTGGGAAATCATGGTGGGCACCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGGCGATCCAGACCGGCCAGCAGATGGGCATGATGACCCTGGACCAGCACCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACCCGGAACCAGGCCAAGGAATACGCCAAGGACAAGCGCATCTTCGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1E7RM02

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.651

99.71

0.794
  pilT Acinetobacter baumannii D1279779

79.651

99.71

0.794
  pilT Acinetobacter baumannii strain A118

79.651

99.71

0.794
  pilT Pseudomonas stutzeri DSM 10701

78.779

99.71

0.786
  pilT Acinetobacter baylyi ADP1

77.907

99.71

0.777
  pilT Pseudomonas aeruginosa PAK

78.466

98.261

0.771
  pilT Legionella pneumophila strain Lp02

74.709

99.71

0.745
  pilT Legionella pneumophila strain ERS1305867

74.709

99.71

0.745
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.821

95.362

0.675
  pilT Vibrio cholerae strain A1552

70.821

95.362

0.675
  pilT Neisseria meningitidis 8013

65.396

98.841

0.646
  pilT Neisseria gonorrhoeae MS11

65.103

98.841

0.643
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.499
  pilU Acinetobacter baylyi ADP1

40.588

98.551

0.4
  pilU Pseudomonas stutzeri DSM 10701

40.413

98.261

0.397
  pilU Vibrio cholerae strain A1552

40.299

97.101

0.391

Multiple sequence alignment