Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GQ674_RS03330 Genome accession   NZ_CP047135
Coordinates   701509..702651 (+) Length   380 a.a.
NCBI ID   WP_159495945.1    Uniprot ID   A0A6P1EEX5
Organism   Stenotrophomonas sp. 364     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 696509..707651
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GQ674_RS03300 (GQ674_03300) soxR 696544..696978 (+) 435 WP_201290209.1 redox-sensitive transcriptional activator SoxR -
  GQ674_RS03305 (GQ674_03305) - 697114..697923 (-) 810 WP_328803873.1 short chain dehydrogenase -
  GQ674_RS03310 (GQ674_03310) - 697814..698695 (+) 882 WP_159495943.1 LysR substrate-binding domain-containing protein -
  GQ674_RS03315 (GQ674_03315) proC 698773..699594 (-) 822 WP_159495944.1 pyrroline-5-carboxylate reductase -
  GQ674_RS03320 (GQ674_03320) - 699626..700306 (-) 681 WP_201290210.1 YggS family pyridoxal phosphate-dependent enzyme -
  GQ674_RS03325 (GQ674_03325) pilT 700393..701430 (+) 1038 WP_070208322.1 type IV pilus twitching motility protein PilT Machinery gene
  GQ674_RS03330 (GQ674_03330) pilU 701509..702651 (+) 1143 WP_159495945.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GQ674_RS03335 (GQ674_03335) - 702824..703408 (+) 585 WP_159495946.1 DUF4234 domain-containing protein -
  GQ674_RS03340 (GQ674_03340) - 703546..704529 (+) 984 WP_159495947.1 aldo/keto reductase -
  GQ674_RS03345 (GQ674_03345) - 704606..705427 (-) 822 WP_159495948.1 alpha/beta hydrolase -
  GQ674_RS03350 (GQ674_03350) - 705599..705817 (+) 219 WP_159495949.1 DUF4287 domain-containing protein -
  GQ674_RS03355 (GQ674_03355) - 705882..706583 (+) 702 WP_159495950.1 hypothetical protein -
  GQ674_RS03360 (GQ674_03360) - 706651..707358 (+) 708 WP_038690005.1 YitT family protein -

Sequence

Protein


Download         Length: 380 a.a.        Molecular weight: 42407.76 Da        Isoelectric Point: 6.8287

>NTDB_id=410917 GQ674_RS03330 WP_159495945.1 701509..702651(+) (pilU) [Stenotrophomonas sp. 364]
MNTTNTTIDFTSFLKLMAHQKASDLFITAGMPPAIKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAI
GLSGVGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNHNSTG
HIITIEDPIEFVHKHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNA
NQAMDRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPSPDGKSRKVAMEIMLGTPLVQDYIRDGEIHKLKEVMKESVQLG
MRTFDQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEVR

Nucleotide


Download         Length: 1143 bp        

>NTDB_id=410917 GQ674_RS03330 WP_159495945.1 701509..702651(+) (pilU) [Stenotrophomonas sp. 364]
ATGAACACCACCAACACGACCATCGACTTCACCTCGTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGACCTGTTCAT
CACCGCGGGCATGCCGCCGGCGATCAAGGTCAACGGCAAGATCTCGCCGATTACCCAGACCCCGCTCACGCCGCAGCAGA
GCCGCGACCTGGTGCTGAACGTGATGACGCCGGCGCAGCGCGAAGAGTTCGAAAAGACCCACGAGTGCAACTTCGCCATC
GGCCTGTCCGGCGTCGGCCGGTTCCGCGTGAGCTGCTTCTACCAGCGCAACCAGGTAGGCATGGTGCTGCGTCGCATCGA
GACGCGCATTCCCACCGTGGAAGAGCTGAGTCTGCCGCCGATCATCAAGACGCTGGCGATGACCAAGCGCGGCATCATTC
TGTTCGTGGGCGCTACCGGTACCGGTAAATCGACTTCGCTGGCGGCGATGATCGGCTACCGCAATCACAACTCGACCGGG
CACATCATCACCATCGAAGACCCGATCGAATTCGTGCACAAGCACGAAGGTTGCATCATCACCCAGCGCGAAGTGGGCAT
CGACACCGACAGCTGGGAAGCAGCGCTGAAGAACACCCTGCGCCAAGCGCCGGACGTGATCATGATCGGCGAGGTGCGCA
CCCGCGAGGGCATGGACCACGCCATCGCCTTCGCCGAAACCGGCCACCTGGTGCTGTGCACGCTGCACGCCAACAACGCC
AACCAGGCGATGGACCGCATCATCAACTTCTTCCCGGAAGACCGCCGCAACCAGCTGCTGATGGATCTGTCGCTGAACCT
CAAGGGCGTGGTGGCGCAGCAGCTGATTCCCTCGCCGGATGGCAAGTCGCGCAAGGTGGCGATGGAGATCATGCTGGGCA
CACCGTTGGTGCAGGATTACATCCGCGATGGCGAGATCCACAAGCTCAAGGAAGTAATGAAGGAGTCGGTGCAGCTGGGC
ATGCGCACCTTCGACCAGAGCCTGTTCGAGCTGTACCAGGCCGGTGAGATCAGCTACGAAGACGCACTGCGCTATGCCGA
TTCGCAGAACGAAGTGCGCCTGCGCATCAAGCTGTCGCAGGGCGGCGACGCACGCACGCTGTCGCAGGGCCTGGACGGGG
TGGAGATCTCGGAAGTCCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(122-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6P1EEX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.575

96.579

0.653
  pilU Acinetobacter baylyi ADP1

63.866

93.947

0.6
  pilU Vibrio cholerae strain A1552

53.371

93.684

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.535

93.421

0.397
  pilT Acinetobacter baumannii strain A118

40.407

90.526

0.366
  pilT Acinetobacter nosocomialis M2

40.407

90.526

0.366
  pilT Acinetobacter baumannii D1279779

40.407

90.526

0.366
  pilT Pseudomonas aeruginosa PAK

40.896

88.158

0.361
  pilT Legionella pneumophila strain Lp02

40.896

88.158

0.361
  pilT Legionella pneumophila strain ERS1305867

40.896

88.158

0.361

Multiple sequence alignment