Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GQS55_RS05470 Genome accession   NZ_CP047130
Coordinates   1256037..1257080 (+) Length   347 a.a.
NCBI ID   WP_159818692.1    Uniprot ID   -
Organism   Colwellia sp. 20A7     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1251037..1262080
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GQS55_RS05445 rdgB 1252294..1252896 (-) 603 WP_159818682.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  GQS55_RS05450 - 1253022..1253462 (-) 441 WP_159818684.1 DUF4426 domain-containing protein -
  GQS55_RS05455 - 1253595..1254140 (-) 546 WP_159818686.1 YggT family protein -
  GQS55_RS05460 proC 1254224..1255042 (-) 819 WP_201294645.1 pyrroline-5-carboxylate reductase -
  GQS55_RS05465 - 1255134..1255892 (-) 759 WP_159818690.1 YggS family pyridoxal phosphate-dependent enzyme -
  GQS55_RS05470 pilT 1256037..1257080 (+) 1044 WP_159818692.1 type IV pilus twitching motility protein PilT Machinery gene
  GQS55_RS05475 pilU 1257114..1258241 (+) 1128 WP_159818694.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GQS55_RS05480 - 1258290..1258724 (-) 435 WP_159818696.1 CBS domain-containing protein -
  GQS55_RS05485 - 1258947..1260392 (+) 1446 WP_159818698.1 coniferyl aldehyde dehydrogenase -
  GQS55_RS05490 - 1260682..1261362 (+) 681 WP_159818700.1 TIGR00153 family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38504.13 Da        Isoelectric Point: 5.4501

>NTDB_id=410837 GQS55_RS05470 WP_159818692.1 1256037..1257080(+) (pilT) [Colwellia sp. 20A7]
MDITELLAFSVQHEASDLHLSTGSPPLIRVDGDVRKLNIPAFDAKDVNALVYDIMNDRQRKEYEEKLEVDFSFEVPNLAR
FRVNAFNQNRGPAAVFRTIPSTILSLDELGCPDIFRDISDTPRGLVLVTGPTGSGKSTTLAAMVDYINDSKNDHILTIED
PIEFVHENKMCLINQREVHRDTLSFEAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
IDVFPAAEKAMVRSMLSESLRAVISQTLIKKVGGGRVAAHEIMIATPAIRNLIREDKIAQMYSSIQTGMSNGMQTMDQCL
QNLVSRGIITKASAMEKAVDKNQFKSF

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=410837 GQS55_RS05470 WP_159818692.1 1256037..1257080(+) (pilT) [Colwellia sp. 20A7]
ATGGATATTACTGAACTACTTGCCTTTAGTGTGCAACATGAAGCATCAGATTTACATTTATCGACCGGGTCGCCACCTTT
GATTCGCGTTGATGGTGATGTTCGTAAACTTAATATACCCGCTTTTGATGCTAAAGATGTTAACGCATTAGTCTACGACA
TTATGAATGACCGTCAGCGTAAAGAATATGAAGAAAAGCTAGAGGTGGATTTTTCTTTTGAAGTGCCTAATTTAGCAAGG
TTTAGGGTTAATGCCTTTAATCAAAATAGGGGGCCTGCCGCTGTTTTTCGTACCATCCCAAGTACAATATTATCCCTTGA
TGAATTAGGTTGTCCTGATATTTTTAGAGATATTTCCGATACCCCACGTGGTTTAGTTTTAGTTACAGGACCAACAGGCT
CGGGTAAGTCGACAACGTTAGCTGCAATGGTTGATTATATTAATGATTCGAAAAACGATCATATTTTAACTATTGAAGAT
CCGATAGAATTTGTACACGAAAATAAAATGTGTTTGATTAACCAACGTGAAGTACATCGTGATACGTTAAGCTTCGAGGC
GGCATTACGTTCAGCTTTACGTGAAGATCCAGATGTTATTCTTGTTGGGGAAATGCGTGATTTAGAAACTATTCGTCTTG
CCATGACTGCCGCTGAAACGGGGCATTTAGTTTTCGGTACCCTACATACAACCTCTGCCCCTAAAACCATTGACCGTATT
ATTGATGTATTCCCTGCAGCAGAAAAAGCAATGGTGCGCTCTATGTTGTCAGAGTCACTTAGAGCCGTTATCTCTCAAAC
GTTAATTAAAAAAGTGGGTGGTGGTCGTGTTGCAGCGCATGAAATTATGATTGCTACACCCGCTATTCGTAATTTAATTC
GTGAAGATAAAATTGCACAAATGTATTCATCTATTCAAACCGGTATGTCTAATGGTATGCAAACGATGGATCAATGTTTA
CAAAATTTAGTTAGTCGAGGCATTATCACTAAAGCCTCAGCCATGGAAAAAGCGGTTGATAAAAATCAGTTCAAAAGCTT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

77.035

99.135

0.764
  pilT Acinetobacter baylyi ADP1

77.035

99.135

0.764
  pilT Pseudomonas aeruginosa PAK

77.219

97.406

0.752
  pilT Acinetobacter nosocomialis M2

75.581

99.135

0.749
  pilT Acinetobacter baumannii D1279779

75.291

99.135

0.746
  pilT Acinetobacter baumannii strain A118

75.291

99.135

0.746
  pilT Legionella pneumophila strain Lp02

73.837

99.135

0.732
  pilT Legionella pneumophila strain ERS1305867

73.837

99.135

0.732
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

73.556

94.813

0.697
  pilT Vibrio cholerae strain A1552

73.556

94.813

0.697
  pilT Neisseria meningitidis 8013

69.322

97.695

0.677
  pilT Neisseria gonorrhoeae MS11

69.027

97.695

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.913

99.424

0.536
  pilU Vibrio cholerae strain A1552

41.618

99.712

0.415
  pilU Acinetobacter baylyi ADP1

40.346

100

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.462

99.712

0.403

Multiple sequence alignment