Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GQS55_RS05475 Genome accession   NZ_CP047130
Coordinates   1257114..1258241 (+) Length   375 a.a.
NCBI ID   WP_159818694.1    Uniprot ID   -
Organism   Colwellia sp. 20A7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1252114..1263241
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GQS55_RS05445 rdgB 1252294..1252896 (-) 603 WP_159818682.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  GQS55_RS05450 - 1253022..1253462 (-) 441 WP_159818684.1 DUF4426 domain-containing protein -
  GQS55_RS05455 - 1253595..1254140 (-) 546 WP_159818686.1 YggT family protein -
  GQS55_RS05460 proC 1254224..1255042 (-) 819 WP_201294645.1 pyrroline-5-carboxylate reductase -
  GQS55_RS05465 - 1255134..1255892 (-) 759 WP_159818690.1 YggS family pyridoxal phosphate-dependent enzyme -
  GQS55_RS05470 pilT 1256037..1257080 (+) 1044 WP_159818692.1 type IV pilus twitching motility protein PilT Machinery gene
  GQS55_RS05475 pilU 1257114..1258241 (+) 1128 WP_159818694.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GQS55_RS05480 - 1258290..1258724 (-) 435 WP_159818696.1 CBS domain-containing protein -
  GQS55_RS05485 - 1258947..1260392 (+) 1446 WP_159818698.1 coniferyl aldehyde dehydrogenase -
  GQS55_RS05490 - 1260682..1261362 (+) 681 WP_159818700.1 TIGR00153 family protein -
  GQS55_RS05495 - 1261373..1262638 (+) 1266 WP_159818702.1 inorganic phosphate transporter -
  GQS55_RS05500 - 1262773..1263054 (-) 282 WP_159818704.1 peptidylprolyl isomerase -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41256.24 Da        Isoelectric Point: 5.5811

>NTDB_id=410838 GQS55_RS05475 WP_159818694.1 1257114..1258241(+) (pilU) [Colwellia sp. 20A7]
MAFHQLLEKMVAAQASDMFVTAKLPVSAKINGELQPIDDQVLTAKDALALVHNAMNEKQIRQFDEEKECNFAISIDGIGR
FRISAFWQRDMAGMVVRRIVTEIPDTDELGLPSVLKDVVMSKRGLVLFVGGTGTGKSTSMASLIGYRNRNSRGHILTIED
PVEFVHEHGKSMITQREVGLDTESFDAALKSSLRQAPDVILIGEIRSKEIMEHALSFAETGHLCIATLHANNANQAIDRI
MHLVPADQHGKLLFDLALNLRGIIAQQLVPTKDGTSRVAAIEILLNSPYVSELIKKGDVGSLKDVMKKSNEQGMQTFDQA
LFNLYEEGLITYADALHHADSPNDLRLMIKLRTNDQSGTGGLRGVTLDGFDDPID

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=410838 GQS55_RS05475 WP_159818694.1 1257114..1258241(+) (pilU) [Colwellia sp. 20A7]
ATGGCCTTTCATCAGTTATTAGAAAAGATGGTTGCTGCACAAGCTTCCGATATGTTTGTTACCGCGAAGTTGCCCGTTAG
TGCTAAAATTAATGGCGAGTTACAACCTATTGATGACCAGGTATTAACTGCCAAAGATGCGTTAGCCTTAGTGCATAATG
CGATGAATGAAAAGCAAATTCGTCAATTTGATGAAGAAAAGGAATGTAACTTTGCCATTTCAATTGATGGGATTGGGCGT
TTTCGTATTTCTGCTTTTTGGCAGCGTGATATGGCAGGTATGGTCGTTCGTCGTATTGTTACTGAAATACCTGATACCGA
CGAGCTTGGTTTACCTTCTGTATTGAAAGATGTTGTGATGTCAAAACGAGGGTTAGTACTTTTTGTTGGCGGTACCGGCA
CCGGTAAGTCAACGTCGATGGCATCATTAATTGGTTATCGAAACCGCAATTCACGTGGACATATTCTAACTATTGAAGAT
CCGGTGGAATTTGTTCATGAACATGGAAAAAGTATGATAACTCAACGTGAAGTAGGGTTGGATACTGAGTCGTTTGATGC
TGCCTTAAAAAGTTCATTACGACAAGCACCTGACGTAATTCTGATTGGTGAAATTCGTAGTAAGGAAATCATGGAGCACG
CGTTAAGTTTTGCTGAAACAGGCCATTTATGTATTGCTACTTTACATGCGAACAACGCAAACCAAGCGATTGATCGAATT
ATGCATTTAGTACCAGCTGATCAGCATGGTAAGTTACTGTTTGATTTAGCATTGAACCTTCGGGGAATTATTGCTCAGCA
ATTGGTACCGACTAAAGACGGTACTAGCCGAGTAGCTGCGATTGAAATTTTATTAAACTCGCCTTATGTCTCAGAGCTTA
TTAAAAAAGGTGATGTTGGTAGTCTCAAAGACGTAATGAAAAAATCTAATGAGCAAGGGATGCAAACCTTTGATCAAGCA
TTATTTAATTTATATGAAGAGGGCTTGATCACATATGCCGATGCACTGCATCATGCTGATTCACCCAATGATTTACGTTT
GATGATTAAATTGCGAACTAATGATCAAAGTGGAACAGGTGGATTGAGAGGTGTAACACTTGATGGCTTTGACGACCCTA
TCGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.358

99.733

0.592
  pilU Acinetobacter baylyi ADP1

55.172

100

0.555
  pilU Vibrio cholerae strain A1552

54.348

98.133

0.533
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.516

92.533

0.403
  pilT Pseudomonas aeruginosa PAK

39.71

92

0.365
  pilT Acinetobacter baumannii D1279779

39.474

91.2

0.36
  pilT Acinetobacter baumannii strain A118

39.474

91.2

0.36
  pilT Acinetobacter nosocomialis M2

39.474

91.2

0.36

Multiple sequence alignment