Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GQR89_RS02810 Genome accession   NZ_CP047024
Coordinates   677605..678648 (+) Length   347 a.a.
NCBI ID   WP_158768655.1    Uniprot ID   -
Organism   Paraglaciecola sp. L1A13     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 672605..683648
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GQR89_RS02785 - 673624..674931 (-) 1308 WP_158768650.1 PotD/PotF family extracellular solute-binding protein -
  GQR89_RS02790 - 675019..675426 (-) 408 WP_233269144.1 DUF4426 domain-containing protein -
  GQR89_RS02795 - 675449..675991 (-) 543 WP_158768652.1 YggT family protein -
  GQR89_RS02800 proC 676016..676837 (-) 822 WP_158768653.1 pyrroline-5-carboxylate reductase -
  GQR89_RS02805 - 676893..677576 (-) 684 WP_158768654.1 YggS family pyridoxal phosphate-dependent enzyme -
  GQR89_RS02810 pilT 677605..678648 (+) 1044 WP_158768655.1 type IV pilus twitching motility protein PilT Machinery gene
  GQR89_RS02815 pilU 678671..679783 (+) 1113 WP_158768656.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GQR89_RS02820 - 679814..680122 (+) 309 WP_158768657.1 DUF2007 domain-containing protein -
  GQR89_RS02825 xerD 680112..681017 (+) 906 WP_158768658.1 site-specific tyrosine recombinase XerD -
  GQR89_RS02830 dsbC 681169..681903 (+) 735 WP_158768659.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38607.35 Da        Isoelectric Point: 6.5667

>NTDB_id=409939 GQR89_RS02810 WP_158768655.1 677605..678648(+) (pilT) [Paraglaciecola sp. L1A13]
MDITELLAFSVKNNASDLHLSAGLPPIIRVDGEMRRLNVPALDHKQVHALIYEIMNDNQRKEYEENLETDFSFEVKDLSR
FRVNAFMQNRGAAAVLRTIPSNILSLDDLGAPQIFKDIINQPTGIVLVTGATGSGKSTTLAAMVDHINRHKREHILTIED
PIEFVHENKLCVVNQREVHRDTHSFSNALRSALREDPDVILVGELRDLETIRLAISAAETGHLVFGTLHTNSAPKTIDRL
IDVFPAEEKSMVRSMLSESLRAVISQTLLKKVGGGRVAAHEIMLGIPAIRNLIREDKVPQMYSVMQTSQAHGMQTMEQCL
KKLVAQGVITNEDAAALSIDKKPTINY

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=409939 GQR89_RS02810 WP_158768655.1 677605..678648(+) (pilT) [Paraglaciecola sp. L1A13]
TTGGATATTACCGAACTTTTAGCCTTTAGTGTCAAAAATAACGCATCAGATTTACATCTTTCCGCAGGTTTGCCGCCGAT
TATACGGGTTGATGGAGAAATGCGTCGGCTAAATGTTCCGGCCTTAGATCACAAACAAGTTCACGCTCTCATTTATGAAA
TCATGAATGATAATCAGCGCAAAGAATATGAAGAAAACTTAGAAACTGACTTTTCTTTTGAAGTAAAAGATTTATCACGT
TTTAGGGTAAACGCTTTTATGCAGAATCGTGGCGCAGCGGCGGTGTTAAGAACCATTCCCAGTAATATTCTGAGTCTAGA
TGATTTAGGTGCGCCACAAATTTTCAAAGATATTATTAATCAGCCCACGGGCATAGTGTTAGTTACTGGTGCAACCGGTT
CGGGGAAAAGTACTACGTTGGCGGCGATGGTCGATCATATTAATCGTCATAAACGTGAGCATATATTAACGATTGAAGAC
CCTATCGAATTTGTGCATGAGAACAAACTTTGCGTAGTGAATCAGCGAGAAGTTCATCGTGATACGCACAGCTTCAGTAA
TGCTTTGCGTTCTGCCTTGCGCGAAGATCCTGACGTTATTTTAGTCGGTGAACTGCGCGATTTAGAAACGATTCGCTTAG
CAATATCCGCTGCTGAAACAGGGCACTTAGTGTTTGGAACGTTACATACCAATTCAGCACCTAAAACGATTGACCGTTTG
ATTGATGTTTTCCCCGCAGAGGAAAAATCCATGGTGCGCTCCATGTTGTCAGAATCATTACGAGCAGTTATTTCGCAAAC
CTTACTTAAAAAAGTCGGGGGGGGACGGGTCGCAGCGCATGAAATTATGCTGGGCATTCCGGCTATTCGAAATCTTATTC
GCGAAGATAAGGTACCGCAGATGTATTCGGTTATGCAGACAAGCCAGGCTCACGGTATGCAAACGATGGAGCAGTGTTTG
AAAAAGTTAGTAGCGCAAGGCGTTATTACCAATGAAGATGCCGCTGCCTTATCTATCGATAAAAAACCGACGATTAATTA
TTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii strain A118

76.347

96.254

0.735
  pilT Acinetobacter baumannii D1279779

76.347

96.254

0.735
  pilT Acinetobacter nosocomialis M2

76.048

96.254

0.732
  pilT Pseudomonas stutzeri DSM 10701

75.749

96.254

0.729
  pilT Acinetobacter baylyi ADP1

74.26

97.406

0.723
  pilT Pseudomonas aeruginosa PAK

74.85

96.254

0.72
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.673

95.965

0.697
  pilT Vibrio cholerae strain A1552

72.673

95.965

0.697
  pilT Legionella pneumophila strain Lp02

70.674

98.271

0.695
  pilT Legionella pneumophila strain ERS1305867

70.674

98.271

0.695
  pilT Neisseria meningitidis 8013

65.373

96.542

0.631
  pilT Neisseria gonorrhoeae MS11

65.075

96.542

0.628
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.187

98.847

0.516
  pilU Vibrio cholerae strain A1552

42.687

96.542

0.412
  pilU Pseudomonas stutzeri DSM 10701

42.388

96.542

0.409
  pilU Acinetobacter baylyi ADP1

41.964

96.83

0.406

Multiple sequence alignment