Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GQR89_RS02815 Genome accession   NZ_CP047024
Coordinates   678671..679783 (+) Length   370 a.a.
NCBI ID   WP_158768656.1    Uniprot ID   -
Organism   Paraglaciecola sp. L1A13     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 673671..684783
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GQR89_RS02790 - 675019..675426 (-) 408 WP_233269144.1 DUF4426 domain-containing protein -
  GQR89_RS02795 - 675449..675991 (-) 543 WP_158768652.1 YggT family protein -
  GQR89_RS02800 proC 676016..676837 (-) 822 WP_158768653.1 pyrroline-5-carboxylate reductase -
  GQR89_RS02805 - 676893..677576 (-) 684 WP_158768654.1 YggS family pyridoxal phosphate-dependent enzyme -
  GQR89_RS02810 pilT 677605..678648 (+) 1044 WP_158768655.1 type IV pilus twitching motility protein PilT Machinery gene
  GQR89_RS02815 pilU 678671..679783 (+) 1113 WP_158768656.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GQR89_RS02820 - 679814..680122 (+) 309 WP_158768657.1 DUF2007 domain-containing protein -
  GQR89_RS02825 xerD 680112..681017 (+) 906 WP_158768658.1 site-specific tyrosine recombinase XerD -
  GQR89_RS02830 dsbC 681169..681903 (+) 735 WP_158768659.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
  GQR89_RS02835 recJ 681979..683697 (+) 1719 WP_158772112.1 single-stranded-DNA-specific exonuclease RecJ -
  GQR89_RS02840 - 683801..684082 (+) 282 WP_158768660.1 hypothetical protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40778.88 Da        Isoelectric Point: 5.4013

>NTDB_id=409940 GQR89_RS02815 WP_158768656.1 678671..679783(+) (pilU) [Paraglaciecola sp. L1A13]
MDLTPYLQEMTDVGASDLFITVGMPISAKINGQITPIGGRKLDEDGAWGLVQDAMSDAQKDQFIQSKECNFAIAREGIGR
FRCSAFWQRDMPGMVIRRIVTEIPQADDLGLPEVLKEIIMSKRGLVLFVGATGTGKSTSLAALIGHRNKHSKGHILTIED
PIEFVHEHQNCVVTQREVGIDTMSFDDALKSSLRQAPDVILIGEIRSMETMEYAMSFADTGHLCVATLHANNANQAIERI
MHLAPPAQHEKLRFDLSLNMRAVIAQQLVPTIDGKGRVAAIEVLLNSPLVTDLIQRNEIGGLKDAMRKGKEMGMQSFDMA
LYSLYQQKIISLEQAIHHADSPNDLRLMIKLDSDNGNTLGSLSNVSIDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=409940 GQR89_RS02815 WP_158768656.1 678671..679783(+) (pilU) [Paraglaciecola sp. L1A13]
ATGGACTTAACCCCGTATTTGCAAGAGATGACTGACGTAGGCGCGTCTGATTTATTTATTACTGTTGGTATGCCTATCAG
CGCCAAAATAAATGGGCAAATCACCCCTATCGGGGGCAGAAAACTTGATGAAGATGGAGCTTGGGGCTTAGTACAAGACG
CCATGTCTGATGCTCAGAAAGACCAATTTATACAGAGCAAAGAGTGTAATTTCGCCATTGCTCGTGAAGGTATTGGGCGT
TTTCGCTGCAGTGCTTTTTGGCAGCGAGACATGCCTGGCATGGTGATACGACGTATCGTGACCGAAATCCCCCAAGCGGA
TGATCTTGGTCTCCCAGAAGTGCTTAAAGAGATCATTATGAGTAAGCGGGGTTTGGTGCTATTTGTTGGTGCTACTGGTA
CCGGTAAGTCAACATCGTTGGCGGCGCTTATTGGGCATAGAAATAAACACTCAAAAGGGCATATTTTAACCATTGAAGAT
CCTATTGAATTTGTCCATGAGCATCAAAACTGTGTGGTAACACAAAGGGAAGTCGGCATTGATACAATGTCATTCGATGA
CGCATTGAAAAGCTCATTGCGCCAAGCGCCAGATGTGATTTTAATTGGCGAAATTCGCTCCATGGAAACCATGGAGTATG
CCATGTCGTTTGCTGATACGGGGCATTTATGTGTGGCAACTCTGCACGCCAATAATGCTAATCAAGCTATTGAGCGGATT
ATGCATTTGGCGCCCCCAGCACAACATGAAAAGCTACGTTTTGATCTAAGCTTGAATATGCGCGCTGTTATTGCTCAGCA
ACTCGTGCCAACCATTGATGGAAAAGGCCGTGTTGCGGCAATTGAGGTTTTACTTAATTCACCTTTGGTAACTGATTTGA
TTCAGCGCAATGAAATTGGTGGCTTAAAGGACGCTATGCGTAAAGGTAAAGAAATGGGAATGCAGAGCTTTGATATGGCA
CTGTATAGTTTGTACCAGCAGAAAATAATCAGCTTGGAGCAAGCGATACATCACGCAGATTCCCCCAATGATTTACGTTT
AATGATTAAGCTTGATTCTGACAACGGCAATACACTGGGCTCGTTGTCAAACGTGTCTATCGACATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

58.856

99.189

0.584
  pilU Pseudomonas stutzeri DSM 10701

59.066

98.378

0.581
  pilU Vibrio cholerae strain A1552

57.838

100

0.578
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.771

98.108

0.4
  pilT Legionella pneumophila strain ERS1305867

40.413

91.622

0.37
  pilT Legionella pneumophila strain Lp02

40.413

91.622

0.37
  pilT Pseudomonas aeruginosa PAK

39.71

93.243

0.37
  pilT Pseudomonas stutzeri DSM 10701

39.13

93.243

0.365

Multiple sequence alignment