Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GNX18_RS12780 Genome accession   NZ_CP046948
Coordinates   3125510..3126544 (+) Length   344 a.a.
NCBI ID   WP_138237093.1    Uniprot ID   -
Organism   Microbulbifer sp. SH-1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3120510..3131544
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GNX18_RS12760 (GNX18_12745) - 3120995..3122962 (-) 1968 WP_166218576.1 dynamin family protein -
  GNX18_RS12765 (GNX18_12750) - 3123172..3123777 (-) 606 WP_138237091.1 YggT family protein -
  GNX18_RS12770 (GNX18_12755) proC 3123837..3124664 (-) 828 WP_240914004.1 pyrroline-5-carboxylate reductase -
  GNX18_RS12775 (GNX18_12760) - 3124715..3125410 (-) 696 WP_166218578.1 YggS family pyridoxal phosphate-dependent enzyme -
  GNX18_RS12780 (GNX18_12765) pilT 3125510..3126544 (+) 1035 WP_138237093.1 type IV pilus twitching motility protein PilT Machinery gene
  GNX18_RS12785 (GNX18_12770) pilU 3126832..3127980 (+) 1149 WP_166218580.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GNX18_RS12790 (GNX18_12775) - 3128166..3128420 (+) 255 WP_166218582.1 hypothetical protein -
  GNX18_RS12795 (GNX18_12780) - 3128417..3129160 (+) 744 WP_166218584.1 SDR family oxidoreductase -
  GNX18_RS12800 (GNX18_12785) - 3129175..3129795 (+) 621 WP_166218586.1 LysE family translocator -
  GNX18_RS12805 (GNX18_12790) - 3129815..3130585 (-) 771 WP_138237098.1 LytTR family DNA-binding domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38456.07 Da        Isoelectric Point: 6.5788

>NTDB_id=409607 GNX18_RS12780 WP_138237093.1 3125510..3126544(+) (pilT) [Microbulbifer sp. SH-1]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDVRRINLPPMEHKQVHGLIYEIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNHNRGAGAVFRTIPSKVLTMEDLGMGQVFKQISDTPRGLVLVTGPTGSGKSTTLAAMIDYINDNKYEHILTIED
PIEFVHESKKCLVNQREVHRDTHGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAQEKSMVRSMLSESLQAVISQTLLKRNGGGRVAAHEIMRGTPAIRNLIREDKIAQMYSAIQTGASVGMQTMDQCL
QDLVERRIVSREVAREKAKMPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=409607 GNX18_RS12780 WP_138237093.1 3125510..3126544(+) (pilT) [Microbulbifer sp. SH-1]
ATGGACATTACAGAACTCCTCGCCTTCAGCGCCAAGCAGAACGCCTCCGACTTGCACCTCTCCGCCGGCCTGCCGCCCAT
GATCCGGGTCGACGGCGACGTCCGCCGCATCAACCTGCCGCCCATGGAGCACAAGCAGGTGCACGGCCTGATCTACGAGA
TCATGAACGACAAGCAGCGCAAGGATTACGAAGAGTTCCTCGAGACCGACTTCTCCTTCGAAGTCCCCGGCGTCGCCCGC
TTCCGGGTCAACGCCTTCAACCACAACCGCGGCGCCGGCGCCGTATTCCGGACCATTCCCTCCAAGGTGCTCACCATGGA
AGACCTGGGAATGGGCCAAGTGTTCAAACAGATCTCCGACACCCCACGCGGCCTGGTCCTCGTCACCGGCCCTACCGGCT
CCGGTAAGTCCACCACCCTCGCCGCGATGATCGACTACATCAACGACAACAAGTACGAACACATCCTCACCATCGAAGAC
CCGATCGAATTCGTACACGAATCGAAAAAGTGCCTGGTCAACCAGCGGGAAGTCCACCGCGACACCCACGGCTTCTCCGA
AGCCCTGCGCTCCGCACTGCGTGAAGACCCCGACATCATCCTCGTCGGCGAGATGCGCGACCTCGAAACCATCCGCCTCG
CCCTCACCGCCGCCGAAACCGGCCACCTCGTCTTCGGCACCCTGCACACCACCTCCGCCGCCAAAACCATCGACCGTGTG
GTGGACGTATTCCCCGCGCAGGAAAAGTCCATGGTGCGCTCCATGCTCTCCGAATCCCTGCAGGCAGTAATCTCTCAGAC
CCTGCTCAAGCGCAACGGCGGCGGCCGCGTCGCCGCCCACGAAATCATGCGCGGCACCCCCGCCATCCGTAACCTAATCC
GCGAAGACAAAATCGCGCAGATGTACTCCGCCATCCAGACCGGCGCCAGCGTCGGCATGCAGACCATGGACCAGTGCCTG
CAGGACCTGGTGGAACGGCGCATCGTCAGCCGTGAAGTGGCGCGTGAGAAGGCGAAGATGCCGGAGAACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

88.953

100

0.89
  pilT Pseudomonas stutzeri DSM 10701

88.081

100

0.881
  pilT Acinetobacter nosocomialis M2

83.721

100

0.837
  pilT Acinetobacter baumannii D1279779

83.721

100

0.837
  pilT Acinetobacter baumannii strain A118

83.721

100

0.837
  pilT Acinetobacter baylyi ADP1

79.651

100

0.797
  pilT Legionella pneumophila strain Lp02

75.444

98.256

0.741
  pilT Legionella pneumophila strain ERS1305867

75.444

98.256

0.741
  pilT Neisseria meningitidis 8013

68.116

100

0.683
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.824

98.837

0.68
  pilT Vibrio cholerae strain A1552

68.824

98.837

0.68
  pilT Neisseria gonorrhoeae MS11

67.536

100

0.677
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.523
  pilU Pseudomonas stutzeri DSM 10701

40.708

98.547

0.401
  pilU Acinetobacter baylyi ADP1

38.728

100

0.39
  pilU Vibrio cholerae strain A1552

40.615

94.477

0.384

Multiple sequence alignment