Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GNX18_RS12785 Genome accession   NZ_CP046948
Coordinates   3126832..3127980 (+) Length   382 a.a.
NCBI ID   WP_166218580.1    Uniprot ID   A0A6G8DFI7
Organism   Microbulbifer sp. SH-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3121832..3132980
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GNX18_RS12765 (GNX18_12750) - 3123172..3123777 (-) 606 WP_138237091.1 YggT family protein -
  GNX18_RS12770 (GNX18_12755) proC 3123837..3124664 (-) 828 WP_240914004.1 pyrroline-5-carboxylate reductase -
  GNX18_RS12775 (GNX18_12760) - 3124715..3125410 (-) 696 WP_166218578.1 YggS family pyridoxal phosphate-dependent enzyme -
  GNX18_RS12780 (GNX18_12765) pilT 3125510..3126544 (+) 1035 WP_138237093.1 type IV pilus twitching motility protein PilT Machinery gene
  GNX18_RS12785 (GNX18_12770) pilU 3126832..3127980 (+) 1149 WP_166218580.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GNX18_RS12790 (GNX18_12775) - 3128166..3128420 (+) 255 WP_166218582.1 hypothetical protein -
  GNX18_RS12795 (GNX18_12780) - 3128417..3129160 (+) 744 WP_166218584.1 SDR family oxidoreductase -
  GNX18_RS12800 (GNX18_12785) - 3129175..3129795 (+) 621 WP_166218586.1 LysE family translocator -
  GNX18_RS12805 (GNX18_12790) - 3129815..3130585 (-) 771 WP_138237098.1 LytTR family DNA-binding domain-containing protein -
  GNX18_RS12810 (GNX18_12795) - 3130582..3131685 (-) 1104 WP_240914005.1 sensor histidine kinase -

Sequence

Protein


Download         Length: 382 a.a.        Molecular weight: 42596.96 Da        Isoelectric Point: 6.4440

>NTDB_id=409608 GNX18_RS12785 WP_166218580.1 3126832..3127980(+) (pilU) [Microbulbifer sp. SH-1]
MEIERLLQLVTEKGASDLFITAGVPASIKLHGKIVPVSTTALTPEKARELVIGIMNERQKKEFIESKELNFAISLRNVGR
FRVSAFFQRNLVGMVLRRIETKIPTIDGLGLPEQLKELAMTKRGLIIFVGATGTGKSTSLASMIGHRNEHSKGHIISIED
PIEFIHQHRGCIVTQREVGLDTDSFETALKNTLRQAPDVILIGEVRSRETMDHAIAFAETGHLCLCTLHANNANQALDRI
IHFFPADRHQQLWMDLSLNLRAMVAQQLVPTPDGDGRRACLEIMINTPLMSDLIRKGEVHKMKELMKRSVEQGMQTFDQA
LYELYDRGEITYEDALSHADSANDLRLMIKLKSESDAEYLNSAADELSLASDTDGGGGPRIY

Nucleotide


Download         Length: 1149 bp        

>NTDB_id=409608 GNX18_RS12785 WP_166218580.1 3126832..3127980(+) (pilU) [Microbulbifer sp. SH-1]
ATGGAAATCGAACGACTCCTACAACTGGTGACTGAAAAAGGCGCATCCGACCTGTTCATCACCGCCGGTGTGCCCGCCTC
CATCAAACTGCACGGCAAAATCGTCCCCGTCAGCACCACCGCGCTCACCCCCGAAAAAGCCCGCGAACTCGTCATCGGCA
TCATGAACGAGCGGCAGAAAAAAGAGTTCATCGAAAGCAAAGAACTCAACTTCGCCATCTCCCTGCGCAACGTCGGCCGC
TTCCGGGTCTCCGCCTTCTTCCAGCGCAACCTCGTCGGCATGGTGCTGCGCCGCATCGAAACCAAAATCCCCACCATTGA
CGGACTCGGCCTGCCCGAGCAGCTCAAAGAACTGGCCATGACCAAGCGCGGCCTCATCATCTTCGTTGGCGCCACCGGTA
CCGGTAAATCCACCTCCCTCGCCTCCATGATCGGCCACCGCAACGAGCACTCCAAGGGTCATATCATCTCCATCGAAGAC
CCCATCGAGTTTATCCACCAGCACCGCGGCTGTATCGTCACCCAGCGCGAAGTCGGCCTCGACACCGACTCCTTCGAGAC
CGCCCTGAAAAACACCCTGCGCCAGGCCCCCGACGTCATCCTCATCGGCGAGGTGCGCTCGCGCGAAACCATGGACCACG
CCATCGCCTTCGCCGAAACCGGCCACCTGTGCCTGTGCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCCACTTCTTCCCCGCCGACCGGCACCAGCAGCTGTGGATGGACCTGTCACTGAACCTGCGCGCCATGGTTGCCCAGCA
GCTGGTACCGACCCCGGACGGCGACGGCCGCCGCGCCTGTCTCGAGATCATGATCAACACCCCGCTGATGTCGGATCTCA
TCCGCAAGGGCGAAGTGCACAAAATGAAGGAACTGATGAAGCGCTCCGTGGAACAGGGCATGCAGACCTTCGACCAGGCG
TTGTACGAACTCTACGACCGCGGCGAGATCACCTATGAAGACGCGCTGTCGCACGCCGACTCCGCCAACGACCTGCGCCT
GATGATCAAGCTCAAGTCCGAGTCCGACGCCGAGTACCTCAACAGCGCCGCCGACGAACTGAGCCTCGCCAGCGATACCG
ATGGCGGCGGCGGCCCGCGCATTTACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(50-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G8DFI7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

75.332

98.691

0.743
  pilU Acinetobacter baylyi ADP1

68.067

93.455

0.636
  pilU Vibrio cholerae strain A1552

55.618

93.194

0.518
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.838

88.743

0.398
  pilT Vibrio cholerae strain A1552

42.136

88.22

0.372
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.136

88.22

0.372
  pilT Legionella pneumophila strain Lp02

41.791

87.696

0.366
  pilT Legionella pneumophila strain ERS1305867

41.791

87.696

0.366
  pilT Acinetobacter nosocomialis M2

40.708

88.743

0.361
  pilT Acinetobacter baumannii D1279779

40.708

88.743

0.361
  pilT Acinetobacter baumannii strain A118

40.708

88.743

0.361

Multiple sequence alignment