Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   METME_RS21460 Genome accession   NC_015572
Coordinates   4711064..4712101 (+) Length   345 a.a.
NCBI ID   WP_013820838.1    Uniprot ID   G0A2W4
Organism   Methylomonas methanica MC09     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4706064..4717101
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  METME_RS21440 (Metme_4269) - 4706927..4707895 (-) 969 WP_013820834.1 GDP-L-fucose synthase -
  METME_RS21445 (Metme_4270) gmd 4707900..4709021 (-) 1122 WP_013820835.1 GDP-mannose 4,6-dehydratase -
  METME_RS21450 (Metme_4271) - 4709194..4710423 (-) 1230 WP_013820836.1 NADP-dependent isocitrate dehydrogenase -
  METME_RS21455 (Metme_4272) - 4710609..4710950 (+) 342 WP_013820837.1 TraR/DksA family transcriptional regulator -
  METME_RS21460 (Metme_4273) pilT 4711064..4712101 (+) 1038 WP_013820838.1 type IV pilus twitching motility protein PilT Machinery gene
  METME_RS21465 (Metme_4274) pilU 4712116..4713249 (+) 1134 WP_013820839.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  METME_RS21470 (Metme_4275) mltB 4713263..4714420 (+) 1158 WP_013820840.1 lytic murein transglycosylase B -
  METME_RS21475 (Metme_4276) - 4714540..4714908 (+) 369 WP_013820841.1 hypothetical protein -
  METME_RS21480 (Metme_4277) - 4715071..4715874 (-) 804 WP_013820842.1 hypothetical protein -
  METME_RS23680 (Metme_4278) - 4716341..4717027 (+) 687 WP_013820843.1 response regulator transcription factor -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38170.97 Da        Isoelectric Point: 6.8400

>NTDB_id=40935 METME_RS21460 WP_013820838.1 4711064..4712101(+) (pilT) [Methylomonas methanica MC09]
MDIAELLTFSVKNKASDLHLSAGLPPMIRVDGDIRRINIPALDHKEVHALIYDIMNDKQRRDYEEFLETDFSFALPGVAR
FRVNAFNQDRGAGAVFRTIPSKVLTLEELGAPKFFEEVCTKPRGLILVTGPTGSGKSTTLAAMINHINNSDYSHILTVED
PIEFVHESQKSLINQREVHRDTLGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPAAEKDMIRSMLSESLQAVISQTLLKKVGGGRIAAHEIMVGTAAIRNLIREAKVAQMYSAIQTGRKDGMQTLDQNL
KELVDKGLVTAKAAMVKAVNKDLFR

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=40935 METME_RS21460 WP_013820838.1 4711064..4712101(+) (pilT) [Methylomonas methanica MC09]
ATGGACATCGCCGAATTATTGACTTTTTCCGTAAAAAACAAAGCCTCGGATTTACATTTGTCCGCCGGTTTGCCGCCCAT
GATTCGGGTCGATGGCGATATCCGCCGTATCAATATTCCGGCCTTGGATCACAAAGAAGTTCACGCGTTGATTTACGACA
TCATGAACGACAAACAGCGCCGCGATTACGAAGAATTCCTGGAAACCGATTTTTCGTTTGCACTACCCGGCGTGGCGCGT
TTCCGGGTCAACGCCTTCAATCAGGACAGGGGCGCCGGCGCCGTGTTCCGGACCATTCCGTCCAAGGTATTAACCCTGGA
AGAGCTGGGCGCACCCAAATTTTTCGAGGAAGTGTGTACCAAACCGCGCGGCCTAATTTTGGTGACAGGCCCTACCGGCT
CCGGCAAATCCACCACGCTGGCCGCCATGATCAACCATATCAACAATAGCGATTATTCGCATATCCTCACCGTCGAAGAT
CCGATCGAATTCGTACACGAAAGCCAAAAATCGCTGATCAACCAACGCGAGGTTCATCGTGATACCCTCGGTTTTAACGA
AGCGTTACGCTCGGCGCTACGGGAAGACCCGGACATTATCCTGGTCGGCGAGATGCGGGATTTGGAAACCATTCGACTGG
CGCTTACCGCCGCCGAAACCGGCCACTTGGTGTTCGGCACCCTGCATACCACCTCGGCGGCCAAAACCATAGACCGTATC
ATCGACGTGTTCCCGGCCGCGGAAAAAGATATGATACGCTCCATGTTGTCGGAATCCTTGCAGGCGGTAATTTCGCAAAC
GCTGTTGAAAAAAGTCGGCGGCGGCCGGATTGCCGCGCATGAAATCATGGTCGGCACGGCGGCAATTCGCAACCTGATCC
GCGAAGCCAAGGTGGCGCAAATGTATTCTGCAATTCAAACCGGACGCAAAGACGGCATGCAGACGCTGGACCAAAATCTG
AAAGAATTGGTCGACAAAGGCTTGGTGACCGCCAAAGCCGCCATGGTGAAAGCCGTTAATAAAGACCTGTTCCGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G0A2W4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

79.942

99.71

0.797
  pilT Pseudomonas aeruginosa PAK

81.065

97.971

0.794
  pilT Pseudomonas stutzeri DSM 10701

79.651

99.71

0.794
  pilT Acinetobacter baumannii D1279779

79.07

99.71

0.788
  pilT Acinetobacter nosocomialis M2

79.07

99.71

0.788
  pilT Acinetobacter baumannii strain A118

79.07

99.71

0.788
  pilT Legionella pneumophila strain Lp02

73.547

99.71

0.733
  pilT Legionella pneumophila strain ERS1305867

73.547

99.71

0.733
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.733

97.681

0.681
  pilT Vibrio cholerae strain A1552

69.733

97.681

0.681
  pilT Neisseria gonorrhoeae MS11

66.472

99.42

0.661
  pilT Neisseria meningitidis 8013

66.472

99.42

0.661
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

100

0.51
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397
  pilU Pseudomonas stutzeri DSM 10701

38.728

100

0.388
  pilU Vibrio cholerae strain A1552

40.615

94.203

0.383

Multiple sequence alignment