Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   METME_RS21465 Genome accession   NC_015572
Coordinates   4712116..4713249 (+) Length   377 a.a.
NCBI ID   WP_013820839.1    Uniprot ID   G0A2W5
Organism   Methylomonas methanica MC09     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4707116..4718249
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  METME_RS21445 (Metme_4270) gmd 4707900..4709021 (-) 1122 WP_013820835.1 GDP-mannose 4,6-dehydratase -
  METME_RS21450 (Metme_4271) - 4709194..4710423 (-) 1230 WP_013820836.1 NADP-dependent isocitrate dehydrogenase -
  METME_RS21455 (Metme_4272) - 4710609..4710950 (+) 342 WP_013820837.1 TraR/DksA family transcriptional regulator -
  METME_RS21460 (Metme_4273) pilT 4711064..4712101 (+) 1038 WP_013820838.1 type IV pilus twitching motility protein PilT Machinery gene
  METME_RS21465 (Metme_4274) pilU 4712116..4713249 (+) 1134 WP_013820839.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  METME_RS21470 (Metme_4275) mltB 4713263..4714420 (+) 1158 WP_013820840.1 lytic murein transglycosylase B -
  METME_RS21475 (Metme_4276) - 4714540..4714908 (+) 369 WP_013820841.1 hypothetical protein -
  METME_RS21480 (Metme_4277) - 4715071..4715874 (-) 804 WP_013820842.1 hypothetical protein -
  METME_RS23680 (Metme_4278) - 4716341..4717027 (+) 687 WP_013820843.1 response regulator transcription factor -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 42309.63 Da        Isoelectric Point: 6.3899

>NTDB_id=40936 METME_RS21465 WP_013820839.1 4712116..4713249(+) (pilU) [Methylomonas methanica MC09]
MDFKALLALMVEKKASDLFITAGKPPCMKIDGKVVDVSKNFLTADQSLKVVLSIMEQRQKDEFEHTKECQFAMGVEGLGR
FRVSAFTQRDAAGMVLRRIETHIPSSEDLHLPPVLKELIMYKRGLVMFVGATGTGKSTSLAALIRHRNENSNGHIITIED
PIEFVHQHKGCIITQREVGLDTESYEVALKNTLRQAPDVILIGEVRTKETMQHAITFAETGHLCLCTLHANNANQALDRI
LHFFPEEMHGQIFMDLSLNLRGIVAQQLVKRADGKGRYPAIEILLNTPLVSDLIRKGEVHKLKELMKNSREHGMQTFDQA
LYDLYTAGKISYEDALNAADSRNEVRLMIKLGAENADFDDGDMKLSETDEDNGSLYR

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=40936 METME_RS21465 WP_013820839.1 4712116..4713249(+) (pilU) [Methylomonas methanica MC09]
ATGGACTTCAAAGCACTATTGGCCTTGATGGTGGAAAAAAAGGCATCGGATTTGTTCATCACGGCCGGTAAGCCGCCCTG
CATGAAAATCGACGGCAAAGTAGTGGATGTCTCGAAAAACTTTCTAACAGCCGATCAATCGCTAAAAGTGGTGCTCAGCA
TCATGGAACAACGCCAGAAAGACGAATTCGAACATACCAAGGAATGCCAGTTCGCCATGGGCGTTGAAGGCTTGGGGCGT
TTCAGGGTCAGCGCATTTACCCAACGCGATGCGGCCGGCATGGTGTTGCGCCGTATCGAAACCCATATCCCTTCTTCCGA
AGACCTGCATTTACCGCCGGTCCTGAAAGAGCTGATCATGTACAAACGCGGCCTGGTAATGTTTGTGGGCGCAACCGGTA
CCGGTAAATCCACCTCGTTGGCAGCCTTAATTCGTCACCGCAACGAAAACAGCAACGGTCATATCATTACCATCGAAGAC
CCGATCGAATTTGTACATCAGCATAAAGGCTGCATCATCACGCAACGCGAAGTTGGATTGGATACCGAGTCTTACGAGGT
TGCGCTGAAAAATACCTTGCGGCAAGCGCCGGATGTAATATTAATCGGCGAGGTCAGGACCAAGGAAACCATGCAACACG
CCATTACCTTTGCCGAAACCGGCCATTTGTGTTTATGTACTTTACATGCGAATAATGCCAATCAGGCATTGGACCGAATC
CTGCATTTTTTTCCAGAAGAAATGCACGGGCAAATCTTTATGGATCTGTCGCTGAATTTACGCGGTATCGTCGCTCAACA
GCTCGTCAAGCGCGCCGACGGCAAGGGCCGTTATCCGGCAATTGAAATTTTATTGAATACGCCGCTGGTTTCGGACTTGA
TTCGCAAGGGCGAAGTGCATAAGCTAAAAGAATTAATGAAAAACTCCCGCGAGCACGGCATGCAGACCTTCGATCAGGCC
TTATACGACCTGTATACCGCCGGGAAAATCAGCTACGAAGACGCATTGAATGCGGCCGATTCCCGTAATGAGGTCCGCTT
GATGATCAAACTCGGTGCCGAAAATGCCGATTTCGATGACGGCGACATGAAACTGTCCGAGACCGATGAAGACAACGGCA
GTCTTTACCGATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(34-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G0A2W5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.675

100

0.66
  pilU Acinetobacter baylyi ADP1

62.162

98.143

0.61
  pilU Vibrio cholerae strain A1552

57.714

92.838

0.536
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.678

92.308

0.403
  pilT Legionella pneumophila strain Lp02

43.658

89.92

0.393
  pilT Legionella pneumophila strain ERS1305867

43.658

89.92

0.393
  pilT Acinetobacter nosocomialis M2

41.888

89.92

0.377
  pilT Acinetobacter baumannii D1279779

41.888

89.92

0.377
  pilT Acinetobacter baumannii strain A118

41.888

89.92

0.377
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.617

88.594

0.369
  pilT Vibrio cholerae strain A1552

41.617

88.594

0.369
  pilT Pseudomonas aeruginosa PAK

41.003

89.92

0.369
  pilT Acinetobacter baylyi ADP1

40.176

90.451

0.363

Multiple sequence alignment