Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GQA94_RS01515 Genome accession   NZ_CP046902
Coordinates   332918..333952 (-) Length   344 a.a.
NCBI ID   WP_158186403.1    Uniprot ID   A0A6I6LQ61
Organism   Stutzerimonas stutzeri strain PM101005     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 327918..338952
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GQA94_RS01495 (GQA94_01495) - 329104..329502 (-) 399 WP_158186399.1 TM2 domain-containing protein -
  GQA94_RS01500 (GQA94_01500) - 329639..330850 (+) 1212 WP_158186400.1 putative DNA modification/repair radical SAM protein -
  GQA94_RS01505 (GQA94_01505) - 330850..331692 (+) 843 WP_158186401.1 TIGR03915 family putative DNA repair protein -
  GQA94_RS01510 (GQA94_01510) pilU 331689..332834 (-) 1146 WP_158186402.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GQA94_RS01515 (GQA94_01515) pilT 332918..333952 (-) 1035 WP_158186403.1 type IV pilus twitching motility protein PilT Machinery gene
  GQA94_RS01520 (GQA94_01520) - 334019..334732 (+) 714 WP_158186404.1 YggS family pyridoxal phosphate-dependent enzyme -
  GQA94_RS01525 (GQA94_01525) proC 334745..335572 (+) 828 WP_158186405.1 pyrroline-5-carboxylate reductase -
  GQA94_RS01530 (GQA94_01530) - 335582..336172 (+) 591 WP_158186406.1 YggT family protein -
  GQA94_RS01535 (GQA94_01535) - 336289..338250 (+) 1962 WP_158186407.1 dynamin family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37946.67 Da        Isoelectric Point: 6.8360

>NTDB_id=409214 GQA94_RS01515 WP_158186403.1 332918..333952(-) (pilT) [Stutzerimonas stutzeri strain PM101005]
MDITELLAFSAKQGASDLHLSSGLPPMIRVDGDVRRINLPAMDHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGSGAVFRTIPSKVLTMEDLGMGEVFRKITDVPRGLVLVTGPTGSGKSTTLAAMLDYLNNTKYHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVISQTLLKKVGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSSIQTGGSLGMQTLDACL
KGLLAKGLISRDSAKEKAKQPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=409214 GQA94_RS01515 WP_158186403.1 332918..333952(-) (pilT) [Stutzerimonas stutzeri strain PM101005]
ATGGATATCACAGAGCTGCTGGCCTTCAGTGCCAAGCAGGGTGCGTCGGATTTGCACCTGTCTTCCGGTTTGCCGCCGAT
GATCCGCGTTGACGGCGATGTGCGCCGGATCAACCTCCCCGCGATGGACCACAAGCAGGTTCATGCCCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTTCGAGGAATTCCTCGAAACCGACTTCTCGTTCGAAGTGCCCGGCGTCGCTCGC
TTTCGCGTCAACGCCTTCAATCAGAACCGCGGTTCCGGCGCCGTGTTCCGGACGATTCCCTCCAAGGTCCTGACCATGGA
GGATCTTGGCATGGGCGAGGTCTTCCGCAAGATCACCGACGTGCCGCGTGGGCTGGTCCTGGTCACCGGGCCGACCGGTT
CGGGTAAATCGACAACGCTGGCCGCGATGCTCGATTACCTGAACAACACCAAGTATCACCACATCCTGACCATCGAGGAC
CCGATCGAATTCGTCCACGAATCCAAGAAGTGCCTGGTCAACCAGCGCGAAGTGCATCGAGACACGCTCGGCTTTTCCGA
AGCGTTGCGTTCGGCCCTGCGTGAAGACCCGGACATCATCCTGGTCGGTGAAATGCGTGACCTGGAAACCATCCGCCTGG
CGCTGACCGCAGCGGAAACCGGCCATCTGGTGTTCGGCACTCTGCACACCACCTCGGCGGCCAAGACCATCGACCGGGTG
GTCGACGTGTTTCCCGCGGAAGAGAAGTCGATGGTCCGCTCGATGCTGTCCGAGTCGCTCCAGGCTGTGATTTCACAGAC
ACTGCTGAAGAAGGTCGGTGGTGGCCGGGTCGCCGCGCACGAGATCATGATTGGCACGCCGGCGATCCGAAACCTGATCC
GCGAAGACAAGGTGGCGCAGATGTATTCCTCGATCCAGACCGGCGGTTCGTTGGGCATGCAGACCCTCGATGCCTGCTTG
AAGGGGCTCCTGGCCAAGGGGCTTATTTCGCGCGACAGTGCCAAGGAAAAAGCCAAGCAACCGGAAAATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I6LQ61

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

99.419

100

0.994
  pilT Pseudomonas aeruginosa PAK

93.895

100

0.939
  pilT Acinetobacter baumannii D1279779

83.721

100

0.837
  pilT Acinetobacter baumannii strain A118

83.721

100

0.837
  pilT Acinetobacter nosocomialis M2

83.43

100

0.834
  pilT Acinetobacter baylyi ADP1

83.14

100

0.831
  pilT Legionella pneumophila strain Lp02

73.964

98.256

0.727
  pilT Legionella pneumophila strain ERS1305867

73.964

98.256

0.727
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.843

97.965

0.674
  pilT Vibrio cholerae strain A1552

68.843

97.965

0.674
  pilT Neisseria meningitidis 8013

67.246

100

0.674
  pilT Neisseria gonorrhoeae MS11

66.957

100

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.754

100

0.529
  pilU Vibrio cholerae strain A1552

41.003

98.547

0.404
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.384

0.395
  pilU Acinetobacter baylyi ADP1

39.017

100

0.392

Multiple sequence alignment