Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GQA94_RS01510 Genome accession   NZ_CP046902
Coordinates   331689..332834 (-) Length   381 a.a.
NCBI ID   WP_158186402.1    Uniprot ID   A0A6I6LR42
Organism   Stutzerimonas stutzeri strain PM101005     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 326689..337834
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GQA94_RS01485 (GQA94_01485) - 326766..327782 (+) 1017 WP_158186397.1 aspartate carbamoyltransferase catalytic subunit -
  GQA94_RS01490 (GQA94_01490) - 327782..329053 (+) 1272 WP_158186398.1 dihydroorotase -
  GQA94_RS01495 (GQA94_01495) - 329104..329502 (-) 399 WP_158186399.1 TM2 domain-containing protein -
  GQA94_RS01500 (GQA94_01500) - 329639..330850 (+) 1212 WP_158186400.1 putative DNA modification/repair radical SAM protein -
  GQA94_RS01505 (GQA94_01505) - 330850..331692 (+) 843 WP_158186401.1 TIGR03915 family putative DNA repair protein -
  GQA94_RS01510 (GQA94_01510) pilU 331689..332834 (-) 1146 WP_158186402.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GQA94_RS01515 (GQA94_01515) pilT 332918..333952 (-) 1035 WP_158186403.1 type IV pilus twitching motility protein PilT Machinery gene
  GQA94_RS01520 (GQA94_01520) - 334019..334732 (+) 714 WP_158186404.1 YggS family pyridoxal phosphate-dependent enzyme -
  GQA94_RS01525 (GQA94_01525) proC 334745..335572 (+) 828 WP_158186405.1 pyrroline-5-carboxylate reductase -
  GQA94_RS01530 (GQA94_01530) - 335582..336172 (+) 591 WP_158186406.1 YggT family protein -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 42532.77 Da        Isoelectric Point: 6.3491

>NTDB_id=409213 GQA94_RS01510 WP_158186402.1 331689..332834(-) (pilU) [Stutzerimonas stutzeri strain PM101005]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKILPVNKTPMSPEMTRETVHAVMNEQQRREFAENHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTLEELKLPDVLKKLAMTKRGLVLFVGATGTGKSTSLAAMIGYRNKNSSGHIISIED
PIEFIHQHQSCIVTQREVGIDTDSFDVALKNTLRQAPDVILIGEVRTRETMDYAVAFAETGHLCLATLHANNANQALDRI
INFFPADRQQQVWMDLSLNLKAIVAQQLIPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKSLMKRSTDLGMQTFDQA
LYNLYVQGEITYEDALLHADSSNDLRLMIKLDSETDSEHLSSISQGLTLEVSDDDPGRSFR

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=409213 GQA94_RS01510 WP_158186402.1 331689..332834(-) (pilU) [Stutzerimonas stutzeri strain PM101005]
ATGGAATTCGAGAAACTGTTGCGCCTGATGGTGGAAAAAGGCGGCTCCGATCTGTTCATCACGGCTGGTGTGCCGCCGTC
GATGAAGGTCAACGGCAAGATCCTGCCGGTGAACAAGACGCCGATGTCGCCGGAGATGACCCGCGAAACGGTGCACGCCG
TGATGAACGAGCAGCAACGCCGGGAGTTCGCAGAAAATCATGAATGCAACTTCGCCATCAGTGCGCGGGGCATCGGCCGT
TTTCGCGTCAGCGCCTTCTATCAGCGCAACCTCGCGGGCATGGTGTTGCGCCGTATCGAAACCAACATTCCGACCCTCGA
AGAGCTCAAACTGCCGGATGTATTGAAGAAGCTCGCCATGACCAAGCGCGGCCTGGTGCTCTTCGTTGGGGCGACCGGCA
CCGGCAAGTCGACGTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCAGCGGGCACATCATCTCCATTGAAGAC
CCCATCGAGTTCATCCACCAGCATCAGAGCTGCATCGTCACTCAGCGCGAAGTCGGCATCGACACCGACTCTTTCGATGT
GGCGCTGAAGAACACCCTGCGCCAGGCCCCTGACGTAATTCTTATCGGCGAAGTGCGGACCCGCGAAACCATGGATTATG
CGGTCGCGTTCGCTGAAACCGGCCATCTCTGCCTGGCCACGCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGGATC
ATCAACTTCTTCCCGGCCGATCGGCAACAGCAGGTCTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCTCAGCA
ACTGATCCCGACCCCGGATGGGAAAGGTCGCCGCGCGGTGATCGAGGTGCTGATCAATACCCCGCTCGCAGCGGACCTGA
TCCGCAAGGGCGAGGTACACGAACTCAAGTCGCTGATGAAACGCTCCACCGACCTCGGTATGCAGACCTTCGACCAGGCA
CTCTACAACCTCTATGTGCAGGGCGAGATTACCTACGAAGACGCCTTGCTTCATGCCGACTCGTCCAATGATCTTCGCCT
GATGATCAAGTTGGACTCCGAAACCGATAGTGAGCACCTGTCCAGCATCTCGCAGGGCTTGACCCTGGAGGTCAGTGACG
ACGACCCAGGCCGCAGCTTTCGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I6LR42

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

95.801

100

0.958
  pilU Acinetobacter baylyi ADP1

63.298

98.688

0.625
  pilU Vibrio cholerae strain A1552

59.429

91.864

0.546
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.248

88.976

0.394
  pilT Neisseria meningitidis 8013

41.159

90.551

0.373
  pilT Neisseria gonorrhoeae MS11

41.159

90.551

0.373
  pilT Legionella pneumophila strain Lp02

41.791

87.927

0.367
  pilT Legionella pneumophila strain ERS1305867

41.791

87.927

0.367
  pilT Pseudomonas aeruginosa PAK

41.791

87.927

0.367
  pilT Vibrio cholerae strain A1552

41.42

88.714

0.367
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.42

88.714

0.367

Multiple sequence alignment