Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GPY12_RS02480 Genome accession   NZ_CP046846
Coordinates   510127..511164 (+) Length   345 a.a.
NCBI ID   WP_000350205.1    Uniprot ID   D2YE46
Organism   Vibrio mimicus strain F9458     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 505127..516164
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY12_RS02445 hemW 505219..506403 (-) 1185 WP_148546805.1 radical SAM family heme chaperone HemW -
  GPY12_RS02450 - 506397..506999 (-) 603 WP_055051670.1 XTP/dITP diphosphatase -
  GPY12_RS02455 - 507085..507516 (-) 432 WP_001233589.1 DUF4426 domain-containing protein -
  GPY12_RS02460 yggU 507610..507903 (-) 294 WP_148546806.1 DUF167 family protein YggU -
  GPY12_RS02465 - 507903..508460 (-) 558 WP_005509546.1 YggT family protein -
  GPY12_RS02470 proC 508518..509336 (-) 819 WP_148554592.1 pyrroline-5-carboxylate reductase -
  GPY12_RS02475 - 509391..510101 (-) 711 WP_001256643.1 YggS family pyridoxal phosphate-dependent enzyme -
  GPY12_RS02480 pilT 510127..511164 (+) 1038 WP_000350205.1 type IV pilus twitching motility protein PilT Machinery gene
  GPY12_RS02485 pilU 511179..512285 (+) 1107 WP_158139260.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GPY12_RS02490 - 512373..513170 (+) 798 WP_158139262.1 helix-turn-helix transcriptional regulator -
  GPY12_RS02495 tyrS 513279..514559 (+) 1281 WP_158139264.1 tyrosine--tRNA ligase -
  GPY12_RS02500 ruvX 514599..515021 (-) 423 WP_000091869.1 Holliday junction resolvase RuvX -
  GPY12_RS02505 - 515103..515666 (-) 564 WP_001054774.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38248.81 Da        Isoelectric Point: 6.5572

>NTDB_id=408810 GPY12_RS02480 WP_000350205.1 510127..511164(+) (pilT) [Vibrio mimicus strain F9458]
MDIAELLEFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFTHSDVHRLVFEIMNDAQRSEYEEKLEVDFSFELPNVGR
FRVNAFHQSRGCSAVFRTIPTVIPTLDQLEAPEIFTKIANYEKGLVLVTGPTGSGKSTTLAAMVDYVNAHHNKHILTIED
PIEFVHSNNKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRVGGGRVACHEIMLATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLIARGVVDAQEVQSKIELDLKAF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=408810 GPY12_RS02480 WP_000350205.1 510127..511164(+) (pilT) [Vibrio mimicus strain F9458]
ATGGATATCGCTGAGTTACTGGAATTTAGTGTAAAGCATAACGCGTCAGATCTACATCTTTCTGCAGGTGTTCCACCTAT
GGTACGGATTGATGGTGAAGTTAGGAAGCTCGGCGTGCCTGCTTTTACTCATTCTGACGTACATCGCTTGGTATTTGAAA
TAATGAATGATGCCCAGCGCAGTGAATATGAAGAAAAATTAGAAGTCGATTTTTCTTTTGAATTACCCAACGTTGGCCGC
TTCCGGGTTAACGCGTTTCACCAATCGCGCGGATGCTCTGCGGTATTTCGTACCATTCCGACGGTGATCCCGACGTTAGA
TCAGCTTGAAGCTCCTGAGATTTTTACCAAGATCGCCAATTATGAAAAAGGCTTAGTGCTGGTCACTGGACCTACCGGTT
CGGGTAAATCCACCACGCTCGCGGCGATGGTTGATTACGTGAATGCCCACCATAACAAGCACATTTTGACCATTGAAGAT
CCGATTGAATTTGTACACAGCAACAATAAGTGTTTGATCAACCAACGCGAAGTGCACCGTGATACCCATAGTTTTAAAAA
TGCCCTGCGCTCGGCATTGCGTGAAGACCCAGATGTGATTCTGGTTGGTGAGCTGCGTGACCAAGAAACCATTAGCTTGG
CGCTTACCGCGGCAGAAACGGGTCACTTGGTGTTTGGTACACTGCACACCAGCTCGGCGGCAAAAACCATCGACCGTATT
ATCGACGTGTTTCCCGGTAGCGATAAAGACATGGTGCGCTCAATGCTTTCTGAATCTCTGCGTGCAGTTATCGCACAAAA
GCTACTGAAACGAGTTGGTGGTGGCCGTGTTGCTTGCCATGAAATCATGCTGGCGACTCCGGCAATCCGTAACTTGATCC
GTGAAGATAAGGTGGCGCAGATGTATTCGATCATCCAAACCGGTGCCGCTCATGGCATGCAGACGATGGAGCAAAATGCG
AAGCAGCTGATTGCGCGTGGTGTAGTGGATGCGCAAGAAGTACAAAGCAAAATTGAGTTGGATTTAAAGGCATTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D2YE46

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

98.261

100

0.983
  pilT Vibrio cholerae strain A1552

98.261

100

0.983
  pilT Acinetobacter baumannii D1279779

72.024

97.391

0.701
  pilT Acinetobacter baumannii strain A118

72.024

97.391

0.701
  pilT Acinetobacter nosocomialis M2

71.726

97.391

0.699
  pilT Acinetobacter baylyi ADP1

72.121

95.652

0.69
  pilT Pseudomonas stutzeri DSM 10701

68.546

97.681

0.67
  pilT Pseudomonas aeruginosa PAK

66.471

98.551

0.655
  pilT Legionella pneumophila strain Lp02

67.477

95.362

0.643
  pilT Legionella pneumophila strain ERS1305867

67.477

95.362

0.643
  pilT Neisseria meningitidis 8013

64.242

95.652

0.614
  pilT Neisseria gonorrhoeae MS11

63.939

95.652

0.612
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.294

94.783

0.496
  pilU Vibrio cholerae strain A1552

41.916

96.812

0.406
  pilU Pseudomonas stutzeri DSM 10701

40.12

96.812

0.388
  pilU Acinetobacter baylyi ADP1

40.625

92.754

0.377

Multiple sequence alignment