Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GPY12_RS02485 Genome accession   NZ_CP046846
Coordinates   511179..512285 (+) Length   368 a.a.
NCBI ID   WP_158139260.1    Uniprot ID   -
Organism   Vibrio mimicus strain F9458     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 506179..517285
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY12_RS02450 - 506397..506999 (-) 603 WP_055051670.1 XTP/dITP diphosphatase -
  GPY12_RS02455 - 507085..507516 (-) 432 WP_001233589.1 DUF4426 domain-containing protein -
  GPY12_RS02460 yggU 507610..507903 (-) 294 WP_148546806.1 DUF167 family protein YggU -
  GPY12_RS02465 - 507903..508460 (-) 558 WP_005509546.1 YggT family protein -
  GPY12_RS02470 proC 508518..509336 (-) 819 WP_148554592.1 pyrroline-5-carboxylate reductase -
  GPY12_RS02475 - 509391..510101 (-) 711 WP_001256643.1 YggS family pyridoxal phosphate-dependent enzyme -
  GPY12_RS02480 pilT 510127..511164 (+) 1038 WP_000350205.1 type IV pilus twitching motility protein PilT Machinery gene
  GPY12_RS02485 pilU 511179..512285 (+) 1107 WP_158139260.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GPY12_RS02490 - 512373..513170 (+) 798 WP_158139262.1 helix-turn-helix transcriptional regulator -
  GPY12_RS02495 tyrS 513279..514559 (+) 1281 WP_158139264.1 tyrosine--tRNA ligase -
  GPY12_RS02500 ruvX 514599..515021 (-) 423 WP_000091869.1 Holliday junction resolvase RuvX -
  GPY12_RS02505 - 515103..515666 (-) 564 WP_001054774.1 YqgE/AlgH family protein -
  GPY12_RS02510 gshB 515702..516658 (-) 957 WP_000593303.1 glutathione synthase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41335.41 Da        Isoelectric Point: 7.1149

>NTDB_id=408811 GPY12_RS02485 WP_158139260.1 511179..512285(+) (pilU) [Vibrio mimicus strain F9458]
MELNQYLDGMLSHKASDLYITVGAPILYRIDGELRAQGEVLSLADVTTLLHAMMDDARQAEFKQTREANFAVVRESGRFR
VSAFFQRELPGAVIRRIETRIPTFEELKLPAVLQNLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNQHRTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMQYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQREQFLLDLSLNLKGVIGQQLLRDKNGKGRHGVFEVLLNSPRVADLIRRGDLHELKTTMARSKEVGMQTFDQALY
QLVVDDKISEQDALHSADSANDLRLMLKTKRGDNYASGSLQNVKIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=408811 GPY12_RS02485 WP_158139260.1 511179..512285(+) (pilU) [Vibrio mimicus strain F9458]
ATGGAGTTGAATCAATATCTGGATGGGATGCTGAGCCATAAAGCATCGGATCTTTACATCACTGTTGGTGCGCCGATTTT
ATATCGGATCGATGGTGAATTGCGCGCGCAAGGTGAAGTGCTCAGTTTGGCGGATGTGACGACCTTACTGCATGCGATGA
TGGATGACGCGAGACAAGCGGAATTTAAGCAGACGCGTGAAGCGAACTTTGCTGTAGTACGTGAAAGTGGACGTTTCCGG
GTTAGTGCGTTTTTCCAACGTGAATTGCCGGGAGCGGTGATCCGCCGGATTGAAACGCGTATTCCCACCTTTGAAGAGCT
GAAATTACCCGCGGTACTGCAAAATTTAGCGATCGCGAAACGCGGTTTAGTTTTGGTGGTAGGCGCAACCGGTTCGGGTA
AATCGACCACTATGGCGGCAATGACGGGGTATCGTAACCAACACCGTACTGGACATATCTTGACGGTCGAAGATCCGATT
GAGTTTGTGCATGAACATAAGCGTTGTATCGTCACCCAACGAGAAGTCGGCTTAGATACTGAAAGCTATGAAGTGGCGCT
GAAAAACTCGCTGCGCCAAGCACCGGATATGATTTTGATTGGTGAAATTCGCAGCCGAGAAACCATGCAATACGCGATGA
CCTTCGCGGAAACGGGACATTTGTGTATGGCCACACTGCACGCCAACAACGCCAACCAAGCTTTAGAGCGGATTTTGCAT
TTAGTGCCGAAAGAACAGCGTGAGCAGTTCTTGCTCGACTTGTCACTCAACCTAAAAGGGGTGATTGGCCAGCAACTGTT
GCGCGATAAAAACGGCAAAGGGCGACATGGGGTGTTTGAAGTTCTGCTCAATAGCCCACGAGTCGCGGATTTGATCCGCC
GTGGCGATCTGCATGAGCTTAAAACGACGATGGCGCGTTCCAAAGAAGTAGGCATGCAGACTTTCGACCAAGCTTTGTAT
CAACTGGTGGTGGACGACAAAATCAGCGAGCAAGATGCCCTGCACAGTGCGGATTCGGCTAACGATCTGCGTTTGATGCT
CAAAACCAAGCGTGGTGACAACTACGCCAGCGGTTCCTTGCAGAATGTGAAGATTGATATGGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

95.924

100

0.959
  pilU Pseudomonas stutzeri DSM 10701

58

95.109

0.552
  pilU Acinetobacter baylyi ADP1

54.986

95.38

0.524
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.806

84.239

0.386
  pilT Legionella pneumophila strain ERS1305867

41.593

92.12

0.383
  pilT Legionella pneumophila strain Lp02

41.593

92.12

0.383
  pilT Acinetobacter baumannii D1279779

40.708

92.12

0.375
  pilT Acinetobacter baumannii strain A118

40.708

92.12

0.375
  pilT Acinetobacter nosocomialis M2

40.708

92.12

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.317

90.761

0.375
  pilT Vibrio cholerae strain A1552

41.317

90.761

0.375
  pilT Pseudomonas aeruginosa PAK

40.118

92.12

0.37
  pilT Acinetobacter baylyi ADP1

41.538

88.315

0.367
  pilT Pseudomonas stutzeri DSM 10701

39.528

92.12

0.364

Multiple sequence alignment