Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GPY06_RS14340 Genome accession   NZ_CP046820
Coordinates   1837302..1838408 (+) Length   368 a.a.
NCBI ID   WP_055035053.1    Uniprot ID   -
Organism   Vibrio metoecus strain 2011V-1015     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1832302..1843408
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY06_RS14305 - 1832591..1833193 (-) 603 WP_055031039.1 XTP/dITP diphosphatase -
  GPY06_RS14310 - 1833263..1833694 (-) 432 WP_001233670.1 DUF4426 domain-containing protein -
  GPY06_RS14315 yggU 1833740..1834030 (-) 291 WP_000741919.1 DUF167 family protein YggU -
  GPY06_RS14320 - 1834030..1834587 (-) 558 WP_009356065.1 YggT family protein -
  GPY06_RS14325 proC 1834642..1835460 (-) 819 WP_158120279.1 pyrroline-5-carboxylate reductase -
  GPY06_RS14330 - 1835514..1836224 (-) 711 WP_158120280.1 YggS family pyridoxal phosphate-dependent enzyme -
  GPY06_RS14335 pilT 1836250..1837287 (+) 1038 WP_158120281.1 type IV pilus twitching motility protein PilT Machinery gene
  GPY06_RS14340 pilU 1837302..1838408 (+) 1107 WP_055035053.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GPY06_RS14345 - 1838527..1839324 (+) 798 WP_167517890.1 helix-turn-helix transcriptional regulator -
  GPY06_RS14350 tyrS 1839433..1840713 (+) 1281 WP_158120283.1 tyrosine--tRNA ligase -
  GPY06_RS14355 ruvX 1840751..1841173 (-) 423 WP_029627830.1 Holliday junction resolvase RuvX -
  GPY06_RS14360 - 1841242..1841805 (-) 564 WP_158120284.1 YqgE/AlgH family protein -
  GPY06_RS14365 gshB 1841841..1842797 (-) 957 WP_000593310.1 glutathione synthase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41357.37 Da        Isoelectric Point: 6.7289

>NTDB_id=408373 GPY06_RS14340 WP_055035053.1 1837302..1838408(+) (pilU) [Vibrio metoecus strain 2011V-1015]
MELNQYLDGMLSHKASDLYITVGAPILYRVDGELRAQGEVLSLADVTTLLNAMMDDARQAEFKQTREANFAVVRDSGRFR
VSAFFQRELPGAVIRRIETRIPTFEELKLPEVLQNLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNQHRTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEIALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQREQFLLDLSLNLKGVIGQQLLRDKNGKGRHGVFEVLLNSPRVADLIRRGELHELKTTMARSKEVGMQTFDQALY
QLVVDDKISEQDALHSADSANDLRLMLKTKRGDNYGSGSLQNVKIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=408373 GPY06_RS14340 WP_055035053.1 1837302..1838408(+) (pilU) [Vibrio metoecus strain 2011V-1015]
ATGGAGTTAAATCAATATCTGGATGGGATGCTCAGCCATAAAGCATCGGATCTTTACATCACCGTGGGAGCGCCGATTTT
ATATCGAGTCGATGGTGAATTGCGTGCGCAAGGTGAAGTGCTCAGCTTGGCGGATGTCACCACCTTGTTGAATGCGATGA
TGGATGACGCGCGGCAAGCAGAATTTAAGCAGACGCGTGAAGCGAACTTTGCGGTGGTGCGTGACAGTGGCCGTTTTCGG
GTCAGCGCGTTTTTCCAACGTGAATTGCCTGGCGCGGTGATCCGCCGGATTGAAACGCGTATTCCCACCTTTGAAGAGCT
GAAATTGCCTGAAGTGCTGCAAAATTTAGCGATCGCGAAACGCGGTTTAGTCTTAGTAGTGGGTGCTACGGGGTCAGGTA
AGTCGACCACCATGGCGGCAATGACTGGCTATCGTAATCAGCACCGTACCGGACATATCTTGACAGTTGAAGATCCCATT
GAGTTTGTGCATGAACACAAGCGCTGCATCGTGACTCAACGAGAAGTTGGGCTCGATACTGAAAGCTATGAAATAGCACT
AAAAAACTCGCTGCGCCAAGCGCCGGATATGATTTTGATTGGTGAAATCCGCAGCCGAGAAACCATGGAATACGCGATGA
CTTTCGCGGAAACCGGTCATTTGTGTATGGCCACTTTGCACGCCAATAATGCCAACCAAGCGTTAGAGCGGATTTTGCAT
TTAGTACCGAAAGAGCAGCGTGAGCAGTTCTTGCTCGACCTGTCACTCAACCTAAAAGGGGTGATTGGTCAGCAACTGTT
GCGCGATAAAAACGGCAAAGGACGACATGGAGTGTTTGAAGTGTTGCTCAATAGTCCACGAGTCGCGGATTTGATTCGCC
GTGGCGAACTGCATGAGCTGAAAACGACCATGGCGCGTTCCAAAGAAGTCGGCATGCAGACCTTCGACCAAGCCCTATAC
CAGCTAGTGGTGGATGACAAAATTAGTGAGCAAGATGCCCTGCACAGTGCCGATTCGGCCAACGATCTGCGTTTGATGCT
CAAAACCAAGCGTGGTGATAACTACGGCAGCGGCTCTTTGCAGAATGTGAAGATTGATATGGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

97.011

100

0.97
  pilU Pseudomonas stutzeri DSM 10701

58

95.109

0.552
  pilU Acinetobacter baylyi ADP1

54.701

95.38

0.522
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.129

84.239

0.389
  pilT Legionella pneumophila strain Lp02

41.888

92.12

0.386
  pilT Legionella pneumophila strain ERS1305867

41.888

92.12

0.386
  pilT Acinetobacter baumannii D1279779

41.003

92.12

0.378
  pilT Acinetobacter baumannii strain A118

41.003

92.12

0.378
  pilT Acinetobacter nosocomialis M2

41.003

92.12

0.378
  pilT Pseudomonas aeruginosa PAK

40.708

92.12

0.375
  pilT Vibrio cholerae strain A1552

41.317

90.761

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.317

90.761

0.375
  pilT Pseudomonas stutzeri DSM 10701

40.118

92.12

0.37
  pilT Acinetobacter baylyi ADP1

41.846

88.315

0.37
  pilT Neisseria gonorrhoeae MS11

38.329

94.293

0.361
  pilT Neisseria meningitidis 8013

38.551

93.75

0.361

Multiple sequence alignment