Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GPY06_RS14335 Genome accession   NZ_CP046820
Coordinates   1836250..1837287 (+) Length   345 a.a.
NCBI ID   WP_158120281.1    Uniprot ID   -
Organism   Vibrio metoecus strain 2011V-1015     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1831250..1842287
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY06_RS14300 hemW 1831422..1832597 (-) 1176 WP_158120278.1 radical SAM family heme chaperone HemW -
  GPY06_RS14305 - 1832591..1833193 (-) 603 WP_055031039.1 XTP/dITP diphosphatase -
  GPY06_RS14310 - 1833263..1833694 (-) 432 WP_001233670.1 DUF4426 domain-containing protein -
  GPY06_RS14315 yggU 1833740..1834030 (-) 291 WP_000741919.1 DUF167 family protein YggU -
  GPY06_RS14320 - 1834030..1834587 (-) 558 WP_009356065.1 YggT family protein -
  GPY06_RS14325 proC 1834642..1835460 (-) 819 WP_158120279.1 pyrroline-5-carboxylate reductase -
  GPY06_RS14330 - 1835514..1836224 (-) 711 WP_158120280.1 YggS family pyridoxal phosphate-dependent enzyme -
  GPY06_RS14335 pilT 1836250..1837287 (+) 1038 WP_158120281.1 type IV pilus twitching motility protein PilT Machinery gene
  GPY06_RS14340 pilU 1837302..1838408 (+) 1107 WP_055035053.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GPY06_RS14345 - 1838527..1839324 (+) 798 WP_167517890.1 helix-turn-helix transcriptional regulator -
  GPY06_RS14350 tyrS 1839433..1840713 (+) 1281 WP_158120283.1 tyrosine--tRNA ligase -
  GPY06_RS14355 ruvX 1840751..1841173 (-) 423 WP_029627830.1 Holliday junction resolvase RuvX -
  GPY06_RS14360 - 1841242..1841805 (-) 564 WP_158120284.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38292.93 Da        Isoelectric Point: 6.5572

>NTDB_id=408372 GPY06_RS14335 WP_158120281.1 1836250..1837287(+) (pilT) [Vibrio metoecus strain 2011V-1015]
MDIAELLEFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFTHSDVHRLIFEIMNDAQRSEYEEKLEVDFSFELPNVGR
FRVNAFHQARGCSAVFRTIPTVIPTLEQLDAPEIFTKIANYEKGLVLVTGPTGSGKSTTLAAMVDYVNAHHNKHILTIED
PIEFVHSNNKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRVGGGRVACHEIMLATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLMVRGVVDAQEVQSKIELDLKAF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=408372 GPY06_RS14335 WP_158120281.1 1836250..1837287(+) (pilT) [Vibrio metoecus strain 2011V-1015]
ATGGATATCGCTGAGTTACTGGAATTTAGTGTAAAGCATAACGCGTCAGATCTACATCTTTCCGCCGGTGTTCCGCCTAT
GGTACGGATTGATGGTGAAGTTAGGAAGCTTGGCGTGCCTGCTTTTACCCATTCTGACGTACATCGCTTGATTTTTGAGA
TCATGAATGATGCCCAGCGCAGTGAATATGAAGAAAAATTAGAAGTCGATTTTTCTTTTGAGTTACCCAACGTTGGCCGT
TTCCGGGTTAACGCGTTTCACCAAGCGCGCGGATGCTCTGCGGTGTTTCGTACCATTCCGACCGTGATCCCAACCTTAGA
GCAATTAGATGCACCTGAGATTTTCACCAAGATCGCCAACTATGAAAAAGGCTTAGTGTTGGTCACCGGACCTACCGGTT
CGGGTAAATCGACCACGCTCGCGGCGATGGTCGATTACGTGAATGCTCATCACAATAAGCATATTTTGACTATTGAAGAT
CCGATTGAATTTGTGCACAGCAACAATAAGTGTTTGATCAACCAACGCGAAGTACACCGTGATACTCACAGTTTTAAAAA
TGCCCTGCGCTCGGCACTGCGTGAAGACCCGGATGTGATTCTGGTTGGTGAGCTGCGTGACCAAGAAACGATTAGCTTGG
CGCTTACCGCAGCGGAAACCGGTCACTTGGTGTTTGGTACACTGCACACCAGCTCGGCGGCAAAAACCATTGACCGGATT
ATCGATGTGTTTCCGGGCAGCGATAAAGACATGGTGCGTTCAATGCTGTCTGAATCGCTGCGTGCGGTTATCGCGCAAAA
GCTCTTAAAACGCGTGGGTGGTGGTCGTGTCGCGTGCCATGAAATCATGCTGGCGACTCCGGCAATTCGGAACTTGATCC
GTGAAGATAAAGTGGCGCAGATGTATTCGATCATCCAAACCGGTGCCGCACATGGTATGCAAACCATGGAGCAAAATGCC
AAACAGCTGATGGTGCGTGGTGTGGTCGATGCGCAAGAAGTGCAGAGCAAAATCGAGTTGGATTTAAAAGCATTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

98.841

100

0.988
  pilT Vibrio cholerae strain A1552

98.841

100

0.988
  pilT Acinetobacter baumannii D1279779

71.726

97.391

0.699
  pilT Acinetobacter baumannii strain A118

71.726

97.391

0.699
  pilT Acinetobacter nosocomialis M2

71.726

97.391

0.699
  pilT Acinetobacter baylyi ADP1

72.424

95.652

0.693
  pilT Pseudomonas stutzeri DSM 10701

68.843

97.681

0.672
  pilT Pseudomonas aeruginosa PAK

66.765

98.551

0.658
  pilT Legionella pneumophila strain Lp02

67.781

95.362

0.646
  pilT Legionella pneumophila strain ERS1305867

67.781

95.362

0.646
  pilT Neisseria meningitidis 8013

64.242

95.652

0.614
  pilT Neisseria gonorrhoeae MS11

63.939

95.652

0.612
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.294

94.783

0.496
  pilU Vibrio cholerae strain A1552

42.515

96.812

0.412
  pilU Pseudomonas stutzeri DSM 10701

41.124

97.971

0.403
  pilU Acinetobacter baylyi ADP1

40.625

92.754

0.377

Multiple sequence alignment