Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PSEFU_RS01545 Genome accession   NC_015556
Coordinates   344502..345536 (+) Length   344 a.a.
NCBI ID   WP_013789435.1    Uniprot ID   A0A1Q9XKX3
Organism   Pseudomonas fulva 12-X     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 339502..350536
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSEFU_RS01520 (Psefu_0303) - 339827..341809 (-) 1983 WP_013789430.1 dynamin-like GTPase family protein -
  PSEFU_RS01525 (Psefu_0304) - 341919..342215 (-) 297 WP_013789431.1 DUF167 family protein -
  PSEFU_RS01530 (Psefu_0305) - 342221..342811 (-) 591 WP_013789432.1 YggT family protein -
  PSEFU_RS01535 (Psefu_0306) proC 342823..343641 (-) 819 WP_013789433.1 pyrroline-5-carboxylate reductase -
  PSEFU_RS01540 (Psefu_0307) - 343679..344365 (-) 687 WP_013789434.1 YggS family pyridoxal phosphate-dependent enzyme -
  PSEFU_RS01545 (Psefu_0308) pilT 344502..345536 (+) 1035 WP_013789435.1 type IV pilus twitching motility protein PilT Machinery gene
  PSEFU_RS01550 (Psefu_0309) pilU 345748..346893 (+) 1146 WP_013789436.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PSEFU_RS22640 (Psefu_0310) - 347792..348649 (-) 858 WP_013789437.1 TIGR03915 family putative DNA repair protein -
  PSEFU_RS01565 (Psefu_0311) - 348649..349860 (-) 1212 WP_013789438.1 putative DNA modification/repair radical SAM protein -
  PSEFU_RS01570 (Psefu_0312) - 350001..350399 (+) 399 WP_013789439.1 NINE protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37953.61 Da        Isoelectric Point: 6.8893

>NTDB_id=40756 PSEFU_RS01545 WP_013789435.1 344502..345536(+) (pilT) [Pseudomonas fulva 12-X]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGSGAVFRTIPSKVLSMEDLGMGEIFKKVSDVPRGLVLVTGPTGSGKSTTLAAMLDYINSNKYHHILTVED
PIEFVHESKKCLVNQREVHRDTHGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVISQTLLKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGALGMETLDSCL
KRLVSKGLVSRESAREKAKTPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=40756 PSEFU_RS01545 WP_013789435.1 344502..345536(+) (pilT) [Pseudomonas fulva 12-X]
ATGGATATCACCGAGCTGCTTGCCTTCAGCGCCAAGCAAGGCGCGTCGGACCTGCACCTTTCCGCAGGCTTGCCGCCGAT
GATCCGGGTCGACGGCGATGTGCGCCGGATCAACCTGCCTGCCCTGGATCACAAGCAGGTCCACGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTTCGAAGAATTCCTGGAAACCGACTTCTCGTTCGAAGTGCCCGGCGTGGCGCGC
TTCCGGGTCAACGCCTTCAACCAGAACCGCGGCAGCGGCGCGGTATTCCGGACCATTCCGTCGAAAGTGCTGAGCATGGA
AGACCTCGGCATGGGCGAGATCTTCAAGAAGGTCTCCGACGTGCCGCGCGGGCTGGTGCTGGTCACCGGGCCGACCGGTT
CGGGCAAGTCGACCACCCTGGCGGCGATGCTCGACTACATCAACAGCAACAAGTACCACCACATCCTCACCGTCGAAGAC
CCCATCGAATTCGTTCACGAGTCGAAGAAGTGTCTGGTCAACCAGCGTGAGGTGCACCGCGATACCCACGGCTTCTCCGA
AGCGCTGCGCTCGGCTCTGCGTGAAGACCCGGACATCATCCTGGTCGGCGAGATGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCAGAAACCGGTCACCTGGTGTTCGGCACCCTGCACACCACCTCCGCCGCCAAGACCATCGACCGGGTG
GTCGACGTGTTCCCGGCCGAGGAAAAGTCCATGGTTCGCTCCATGCTGTCCGAATCCCTGCAGGCGGTGATTTCCCAGAC
CCTGCTGAAGAAGATCGGCGGCGGTCGGGTGGCGGCCCACGAAATCATGATCGGCACCCCGGCGATCCGAAACCTGATCC
GCGAAGACAAGGTGGCGCAGATGTATTCGGCCATCCAGACCGGTGGTGCACTGGGTATGGAAACCCTCGACTCCTGCCTG
AAACGGCTGGTGTCCAAAGGCCTGGTCAGCCGTGAAAGTGCCCGAGAGAAGGCCAAGACACCCGAAAACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Q9XKX3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

93.314

100

0.933
  pilT Pseudomonas stutzeri DSM 10701

93.023

100

0.93
  pilT Acinetobacter nosocomialis M2

85.465

100

0.855
  pilT Acinetobacter baumannii D1279779

85.174

100

0.852
  pilT Acinetobacter baumannii strain A118

85.174

100

0.852
  pilT Acinetobacter baylyi ADP1

81.686

100

0.817
  pilT Legionella pneumophila strain Lp02

75.74

98.256

0.744
  pilT Legionella pneumophila strain ERS1305867

75.74

98.256

0.744
  pilT Neisseria meningitidis 8013

66.957

100

0.672
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.546

97.965

0.672
  pilT Vibrio cholerae strain A1552

68.546

97.965

0.672
  pilT Neisseria gonorrhoeae MS11

66.667

100

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

100

0.512
  pilU Vibrio cholerae strain A1552

40

100

0.401
  pilU Acinetobacter baylyi ADP1

39.595

100

0.398
  pilU Pseudomonas stutzeri DSM 10701

40

97.384

0.39

Multiple sequence alignment