Detailed information NTG0040757

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PSEFU_RS01550
• Genome accession: NC_015556
• Coordinates: 345748..346893 (+)
• Length: 381 a.a.
• NCBI ID: WP_013789436.1
• Uniprot ID: F6AFV5
• Organism: Pseudomonas fulva 12-X
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 340748..351893

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PSEFU_RS01525 (Psefu_0304)	-	341919..342215 (-)	297	WP_013789431.1	DUF167 family protein	-
PSEFU_RS01530 (Psefu_0305)	-	342221..342811 (-)	591	WP_013789432.1	YggT family protein	-
PSEFU_RS01535 (Psefu_0306)	proC	342823..343641 (-)	819	WP_013789433.1	pyrroline-5-carboxylate reductase	-
PSEFU_RS01540 (Psefu_0307)	-	343679..344365 (-)	687	WP_013789434.1	YggS family pyridoxal phosphate-dependent enzyme	-
PSEFU_RS01545 (Psefu_0308)	pilT	344502..345536 (+)	1035	WP_013789435.1	type IV pilus twitching motility protein PilT	Machinery gene
PSEFU_RS01550 (Psefu_0309)	pilU	345748..346893 (+)	1146	WP_013789436.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PSEFU_RS22640 (Psefu_0310)	-	347792..348649 (-)	858	WP_013789437.1	TIGR03915 family putative DNA repair protein	-
PSEFU_RS01565 (Psefu_0311)	-	348649..349860 (-)	1212	WP_013789438.1	putative DNA modification/repair radical SAM protein	-
PSEFU_RS01570 (Psefu_0312)	-	350001..350399 (+)	399	WP_013789439.1	NINE protein	-
PSEFU_RS01575 (Psefu_0313)	-	350520..351791 (-)	1272	WP_013789440.1	dihydroorotase	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42552.81 Da
• Isoelectric Point: 6.7258

>NTDB_id=40757 PSEFU_RS01550 WP_013789436.1 345748..346893(+) (pilU) [Pseudomonas fulva 12-X]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVTKTPLSPEMTRETVHGVMNEQQRREFAENHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTLDDLKLPEILKKLALTKRGLVLFVGATGTGKSTSLAAMIGYRNKNSSGHIISIED
PIEYIHQHQNCIVTQREVGIDTDSFEVALKNTLRQAPDVILIGEVRTRETMDHAVAFAETGHLCLATLHANNANQALDRI
INFFPADRQNQVWMDLSLNLKAIVAQQLIPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKGLMKRSTEHGMQTFDQA
LYNLYSQGEITYEDALLYADSANDLRLMIKLGSETDGEHLTSMSQGLALEISDEDQGRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=40757 PSEFU_RS01550 WP_013789436.1 345748..346893(+) (pilU) [Pseudomonas fulva 12-X]
ATGGAATTCGAAAAACTGTTGCGTCTGATGGTCGAGAAGGGCGGCTCCGATCTGTTCATCACCGCTGGCGTGCCGCCGTC
GATGAAGGTCAATGGCAAGATCATGCCCGTGACCAAGACCCCGCTGTCGCCGGAGATGACCCGTGAAACCGTGCACGGGG
TGATGAACGAGCAGCAACGCCGGGAGTTCGCCGAAAACCACGAGTGCAACTTCGCGATCAGCGCCCGTGGCATCGGCCGT
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGCTGGCATGGTGCTGCGCCGCATCGAAACCAACATCCCGACGCTGGA
CGACCTCAAGCTGCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAACGCGGCCTGGTGCTGTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCCCTGGCGGCGATGATCGGCTACCGCAACAAGAACAGCAGCGGCCACATCATCTCCATCGAAGAC
CCGATCGAGTACATCCACCAGCACCAGAACTGCATCGTCACCCAGCGCGAAGTGGGCATCGACACTGATTCTTTCGAGGT
CGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTCATCGGTGAGGTGCGTACCCGCGAGACCATGGACCACG
CCGTGGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGGATC
ATCAACTTCTTCCCGGCGGACCGGCAGAACCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGATCCCCACGCCCGACGGCAAGGGCCGCCGCGCCGTGATCGAGGTGCTGATCAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGTGAGGTGCACGAGCTCAAGGGCCTGATGAAGCGCTCCACCGAGCACGGCATGCAGACCTTCGACCAGGCG
CTTTACAACCTCTACAGCCAGGGCGAGATCACCTACGAAGATGCCCTGCTGTACGCTGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGCTCGGAAACCGACGGCGAACACCTGACCAGCATGTCCCAGGGTCTGGCCTTGGAGATCAGCGACG
AAGATCAGGGGCGGCGGTTTCGTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (110-270)

3D structure

• AlphaFold DB: F6AFV5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	94.488	100	0.945
pilU	Acinetobacter baylyi ADP1	65.826	93.701	0.617
pilU	Vibrio cholerae strain A1552	58.857	91.864	0.541
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.452	88.189	0.383
pilT	Legionella pneumophila strain Lp02	41.493	87.927	0.365
pilT	Legionella pneumophila strain ERS1305867	41.493	87.927	0.365