Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GPX90_RS09720 Genome accession   NZ_CP046752
Coordinates   1014991..1016025 (-) Length   344 a.a.
NCBI ID   WP_106341300.1    Uniprot ID   -
Organism   Photobacterium damselae strain 9046-81     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1009991..1021025
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPX90_RS09695 rsmE 1010707..1011438 (+) 732 WP_005297699.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  GPX90_RS09700 gshB 1011459..1012406 (+) 948 WP_005297697.1 glutathione synthase -
  GPX90_RS09705 - 1012560..1013123 (+) 564 WP_115425630.1 YqgE/AlgH family protein -
  GPX90_RS09710 ruvX 1013120..1013545 (+) 426 WP_005297693.1 Holliday junction resolvase RuvX -
  GPX90_RS09715 pilU 1013873..1014979 (-) 1107 WP_005297691.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GPX90_RS09720 pilT 1014991..1016025 (-) 1035 WP_106341300.1 type IV pilus twitching motility protein PilT Machinery gene
  GPX90_RS09725 - 1016048..1016749 (+) 702 WP_005297686.1 YggS family pyridoxal phosphate-dependent enzyme -
  GPX90_RS09730 proC 1016765..1017583 (+) 819 WP_106224067.1 pyrroline-5-carboxylate reductase -
  GPX90_RS09735 - 1017595..1018152 (+) 558 WP_005297682.1 YggT family protein -
  GPX90_RS09740 yggU 1018149..1018448 (+) 300 WP_036763214.1 DUF167 family protein YggU -
  GPX90_RS09745 - 1018550..1019143 (+) 594 WP_106341299.1 XTP/dITP diphosphatase -
  GPX90_RS09750 hemW 1019143..1020303 (+) 1161 WP_158135303.1 radical SAM family heme chaperone HemW -
  GPX90_RS09755 - 1020378..1020782 (-) 405 WP_050787742.1 DUF2884 family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38194.76 Da        Isoelectric Point: 6.1433

>NTDB_id=407143 GPX90_RS09720 WP_106341300.1 1014991..1016025(-) (pilT) [Photobacterium damselae strain 9046-81]
MDITELLDFSVKHNASDLHLSAGVPPMIRVDGDVRKLSLPALEHSEVHRLIFDIMNDAQRRQFEEELEVDFSFELPDIGR
FRVNAFHQSRGCSAVFRTIPMTIPTLESLNVPDVFYKIAQCQRGLVLVTGATGSGKSTTIAALVDYINEHTNRHILTIED
PIEFVHHSKRCLINQREVHRDTHSFQAALRSALREDPDVIVVGELRDQETISLALTAAETGHLVLGTLHTSSAAKSIDRI
IDVFPGSDKSMVRSMLSESLRAVVSQSLLKCISGGRVASHEIMMATPAIRNLIREDKIAQMYSMIQTGSAMGMQTMEQSV
KMLVAQGLVEAEEGRRIVDSAQQI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=407143 GPX90_RS09720 WP_106341300.1 1014991..1016025(-) (pilT) [Photobacterium damselae strain 9046-81]
ATGGATATCACCGAATTATTAGACTTCAGTGTAAAACATAACGCATCGGATCTGCATCTTTCAGCAGGTGTTCCACCTAT
GATCCGTGTAGATGGTGATGTACGCAAGTTAAGTTTACCTGCATTAGAGCACTCAGAGGTTCATCGTCTGATTTTTGATA
TCATGAATGATGCCCAACGTCGTCAATTTGAAGAAGAGTTAGAAGTCGACTTCTCATTTGAATTACCCGATATTGGCCGT
TTTCGTGTTAACGCTTTTCACCAATCTCGAGGTTGTTCTGCGGTCTTTCGTACTATCCCTATGACCATTCCAACGTTGGA
ATCTCTGAATGTGCCCGATGTTTTCTACAAAATAGCACAATGTCAGCGTGGATTGGTATTAGTTACAGGGGCTACAGGTT
CTGGTAAATCCACCACCATTGCAGCATTGGTTGATTATATTAATGAGCACACCAATCGTCATATCCTCACGATTGAAGAT
CCAATTGAATTTGTGCATCACAGTAAACGTTGTTTGATTAACCAACGAGAAGTGCATCGTGATACCCACAGTTTTCAAGC
CGCTTTGCGCTCGGCATTACGTGAAGATCCCGATGTGATTGTTGTGGGGGAATTGCGAGATCAAGAGACCATCAGTTTAG
CGTTAACTGCTGCAGAAACCGGACACTTGGTTTTAGGTACGCTACACACAAGTTCAGCAGCCAAAAGTATTGACCGTATT
ATCGATGTATTTCCTGGTAGTGATAAATCCATGGTTCGCTCCATGTTATCTGAGTCATTACGTGCAGTGGTTTCCCAAAG
TTTGTTGAAATGCATTAGCGGTGGTCGTGTGGCTTCCCATGAGATCATGATGGCAACCCCTGCGATTCGAAATTTGATCC
GCGAAGATAAAATTGCTCAGATGTATTCCATGATCCAAACCGGATCGGCAATGGGAATGCAGACAATGGAACAGAGTGTG
AAGATGCTGGTTGCTCAAGGACTAGTGGAAGCAGAAGAAGGTCGTCGTATAGTCGACAGTGCCCAACAGATCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

79.58

96.802

0.77
  pilT Vibrio cholerae strain A1552

79.58

96.802

0.77
  pilT Acinetobacter baumannii D1279779

70.149

97.384

0.683
  pilT Acinetobacter baumannii strain A118

70.149

97.384

0.683
  pilT Acinetobacter nosocomialis M2

69.851

97.384

0.68
  pilT Pseudomonas aeruginosa PAK

69.851

97.384

0.68
  pilT Acinetobacter baylyi ADP1

68.421

99.419

0.68
  pilT Pseudomonas stutzeri DSM 10701

69.552

97.384

0.677
  pilT Legionella pneumophila strain Lp02

69.605

95.64

0.666
  pilT Legionella pneumophila strain ERS1305867

69.605

95.64

0.666
  pilT Neisseria meningitidis 8013

65.282

97.965

0.64
  pilT Neisseria gonorrhoeae MS11

64.985

97.965

0.637
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50

100

0.5
  pilU Vibrio cholerae strain A1552

41.159

100

0.413
  pilU Pseudomonas stutzeri DSM 10701

40.87

100

0.41
  pilU Acinetobacter baylyi ADP1

39.13

93.605

0.366

Multiple sequence alignment