Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GPX90_RS09715 Genome accession   NZ_CP046752
Coordinates   1013873..1014979 (-) Length   368 a.a.
NCBI ID   WP_005297691.1    Uniprot ID   A0A2T3I981
Organism   Photobacterium damselae strain 9046-81     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1008873..1019979
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPX90_RS09685 - 1009258..1009740 (+) 483 WP_158135301.1 SprT family zinc-dependent metalloprotease -
  GPX90_RS09690 endA 1009871..1010575 (+) 705 WP_005297700.1 deoxyribonuclease I -
  GPX90_RS09695 rsmE 1010707..1011438 (+) 732 WP_005297699.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  GPX90_RS09700 gshB 1011459..1012406 (+) 948 WP_005297697.1 glutathione synthase -
  GPX90_RS09705 - 1012560..1013123 (+) 564 WP_115425630.1 YqgE/AlgH family protein -
  GPX90_RS09710 ruvX 1013120..1013545 (+) 426 WP_005297693.1 Holliday junction resolvase RuvX -
  GPX90_RS09715 pilU 1013873..1014979 (-) 1107 WP_005297691.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GPX90_RS09720 pilT 1014991..1016025 (-) 1035 WP_106341300.1 type IV pilus twitching motility protein PilT Machinery gene
  GPX90_RS09725 - 1016048..1016749 (+) 702 WP_005297686.1 YggS family pyridoxal phosphate-dependent enzyme -
  GPX90_RS09730 proC 1016765..1017583 (+) 819 WP_106224067.1 pyrroline-5-carboxylate reductase -
  GPX90_RS09735 - 1017595..1018152 (+) 558 WP_005297682.1 YggT family protein -
  GPX90_RS09740 yggU 1018149..1018448 (+) 300 WP_036763214.1 DUF167 family protein YggU -
  GPX90_RS09745 - 1018550..1019143 (+) 594 WP_106341299.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41354.51 Da        Isoelectric Point: 5.5884

>NTDB_id=407142 GPX90_RS09715 WP_005297691.1 1013873..1014979(-) (pilU) [Photobacterium damselae strain 9046-81]
MKLQEILNQVVERKASDLYITVDSPCLLKVDGVLHPIGDTLDRTGVDLLFDEAMDSALYQEFVDTREANFALVRGDWRFR
VSAFWQRELPGMVVRRIETDIPDIFSLKLPMDMQKMALAKRGLVLVVGATGSGKSTSMAAMTGFRNQNRSGHILTVEDPI
EFVHKHDKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRTQETMEYAMNFAETGHLCMATLHANNANQALERILH
LVPKERQHQFLFDLSLNLKCILAQQLIPDAHGQGRHAAFELLINTPRVADLIRRGELYEIKDVMAKSGESGMMTFDQSLY
ELFTKGAITEQDALHHADSPNDLRLMIKVGSKDKTAINALDGVTVDFN

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=407142 GPX90_RS09715 WP_005297691.1 1013873..1014979(-) (pilU) [Photobacterium damselae strain 9046-81]
ATGAAACTACAAGAAATTTTAAATCAGGTTGTTGAACGTAAAGCGTCGGATCTCTATATCACCGTGGATTCTCCGTGCCT
ATTAAAAGTGGATGGTGTGTTGCATCCCATTGGCGATACGTTAGATCGTACAGGGGTTGATTTGCTATTTGATGAGGCGA
TGGACAGTGCTTTATATCAAGAGTTTGTTGATACCCGAGAAGCGAATTTTGCTTTAGTTCGTGGTGATTGGCGGTTTCGT
GTCAGTGCGTTTTGGCAGCGAGAATTACCCGGCATGGTTGTTCGTCGGATTGAAACCGATATTCCAGATATCTTTAGCCT
GAAATTGCCGATGGATATGCAAAAAATGGCTCTAGCTAAACGCGGTTTAGTTTTAGTGGTTGGTGCAACGGGGTCGGGTA
AATCGACTTCGATGGCGGCAATGACCGGGTTTCGAAATCAAAATCGCAGTGGCCATATTTTAACGGTTGAAGATCCGATT
GAATTTGTCCACAAACACGATAAATGCATAGTGACTCAGCGTGAAGTTGGCCTCGATACTGAGAGTTATGAAGTGGCGCT
TAAGAACTCTTTGCGACAAGCGCCTGATATGATCTTAATCGGCGAAATTCGTACGCAAGAGACGATGGAATATGCGATGA
ATTTCGCGGAAACGGGTCATTTATGTATGGCCACATTGCACGCCAATAATGCTAACCAAGCGTTAGAGCGCATTCTGCAT
CTGGTACCGAAAGAGCGCCAACATCAGTTTTTGTTTGATTTATCCTTGAACCTTAAATGCATTTTAGCTCAGCAACTTAT
CCCTGATGCTCATGGTCAAGGACGGCATGCAGCCTTTGAACTGTTAATTAATACACCGCGAGTGGCGGATCTTATTCGTC
GTGGTGAACTTTATGAGATCAAAGATGTGATGGCCAAATCAGGTGAATCGGGAATGATGACTTTTGATCAATCGTTGTAT
GAGTTATTCACTAAAGGTGCCATTACAGAACAAGATGCTCTTCATCATGCGGATTCTCCAAATGATTTACGTTTAATGAT
CAAAGTAGGGAGTAAAGATAAAACGGCGATCAATGCGTTGGATGGGGTTACGGTAGATTTTAATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2T3I981

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

68.12

99.728

0.679
  pilU Pseudomonas stutzeri DSM 10701

59.497

97.283

0.579
  pilU Acinetobacter baylyi ADP1

55.211

96.467

0.533
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.449

93.75

0.389
  pilT Legionella pneumophila strain Lp02

40.299

91.033

0.367
  pilT Legionella pneumophila strain ERS1305867

40.299

91.033

0.367

Multiple sequence alignment