Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GPW70_RS17030 Genome accession   NZ_CP046740
Coordinates   2662223..2663260 (-) Length   345 a.a.
NCBI ID   WP_000350195.1    Uniprot ID   Q9KUQ3
Organism   Vibrio cholerae strain 3566-06     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2657223..2668260
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPW70_RS17005 - 2657693..2658256 (+) 564 WP_019828858.1 YqgE/AlgH family protein -
  GPW70_RS17010 ruvX 2658337..2658759 (+) 423 WP_019828856.1 Holliday junction resolvase RuvX -
  GPW70_RS17015 tyrS 2658798..2660078 (-) 1281 WP_019828854.1 tyrosine--tRNA ligase -
  GPW70_RS17020 - 2660187..2660984 (-) 798 WP_001123226.1 helix-turn-helix transcriptional regulator -
  GPW70_RS17025 pilU 2661102..2662208 (-) 1107 WP_000422572.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GPW70_RS17030 pilT 2662223..2663260 (-) 1038 WP_000350195.1 type IV pilus twitching motility protein PilT Machinery gene
  GPW70_RS17035 - 2663286..2663996 (+) 711 WP_001888157.1 YggS family pyridoxal phosphate-dependent enzyme -
  GPW70_RS17040 proC 2664049..2664867 (+) 819 WP_000437941.1 pyrroline-5-carboxylate reductase -
  GPW70_RS17045 - 2664921..2665478 (+) 558 WP_001087261.1 YggT family protein -
  GPW70_RS17050 yggU 2665478..2665768 (+) 291 WP_000741914.1 DUF167 family protein YggU -
  GPW70_RS17055 - 2665814..2666245 (+) 432 WP_001233675.1 DUF4426 domain-containing protein -
  GPW70_RS17060 - 2666315..2666917 (+) 603 WP_032472721.1 XTP/dITP diphosphatase -
  GPW70_RS17065 hemW 2666911..2668086 (+) 1176 WP_001888158.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38231.83 Da        Isoelectric Point: 6.6672

>NTDB_id=406935 GPW70_RS17030 WP_000350195.1 2662223..2663260(-) (pilT) [Vibrio cholerae strain 3566-06]
MDIAELLEFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFTHSDVHRLIFEIMNDAQRSEYEEKLEVDFSFELPNVGR
FRVNAFHQARGCSAVFRTIPTVIPTLEQLDAPEIFSKIANYEKGLVLVTGPTGSGKSTTLAAMVNYVNAHHNKHILTIED
PIEFVHSNNKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRVGGGRVACHEIMLATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLIARGVVDAQEVQSKIELDLKAF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=406935 GPW70_RS17030 WP_000350195.1 2662223..2663260(-) (pilT) [Vibrio cholerae strain 3566-06]
ATGGATATCGCTGAGTTACTGGAATTTAGTGTAAAACATAACGCCTCAGATCTACATCTTTCCGCAGGTGTTCCGCCTAT
GGTACGGATTGATGGCGAAGTTAGGAAGCTTGGCGTACCTGCTTTTACCCATTCTGATGTACATCGCTTGATTTTTGAGA
TCATGAATGATGCCCAGCGCAGTGAATATGAAGAGAAATTAGAAGTCGATTTTTCTTTTGAACTGCCTAATGTTGGCCGT
TTCCGGGTTAACGCGTTTCATCAAGCGCGCGGATGCTCGGCGGTGTTTCGTACCATTCCCACTGTCATCCCGACGTTAGA
GCAACTGGATGCTCCTGAGATCTTCAGCAAAATTGCCAATTATGAAAAAGGGTTAGTTTTAGTGACTGGGCCAACGGGGT
CGGGTAAGTCGACGACCTTGGCGGCGATGGTGAACTATGTCAATGCCCATCACAATAAGCATATTTTGACTATTGAAGAT
CCGATTGAATTTGTGCACAGCAACAATAAGTGTTTGATCAACCAACGCGAAGTGCACCGTGATACCCACAGTTTTAAAAA
TGCATTGCGCTCGGCATTGCGTGAAGACCCAGATGTGATCCTCGTCGGTGAGCTGCGTGACCAAGAAACGATTAGCTTGG
CGCTTACGGCGGCAGAAACCGGTCACTTGGTGTTTGGCACTCTACACACCAGCTCGGCGGCGAAAACTATTGACCGGATT
ATCGATGTTTTTCCGGGCAGCGACAAAGACATGGTGCGTTCCATGCTGTCGGAATCGCTGCGTGCGGTTATTGCGCAAAA
ACTCCTAAAACGCGTCGGGGGTGGCCGTGTCGCGTGTCATGAAATCATGCTGGCAACGCCAGCGATCCGGAACTTGATCC
GTGAAGATAAAGTGGCGCAGATGTATTCGATCATCCAAACCGGTGCCGCACACGGCATGCAAACCATGGAGCAAAACGCC
AAACAGCTGATCGCTCGTGGTGTGGTGGATGCGCAAGAAGTACAGAGCAAAATCGAGTTGGATTTAAAAGCATTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9KUQ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

100

100

1
  pilT Vibrio cholerae strain A1552

100

100

1
  pilT Acinetobacter baumannii D1279779

71.726

97.391

0.699
  pilT Acinetobacter baumannii strain A118

71.726

97.391

0.699
  pilT Acinetobacter nosocomialis M2

71.429

97.391

0.696
  pilT Acinetobacter baylyi ADP1

72.424

95.652

0.693
  pilT Pseudomonas stutzeri DSM 10701

68.843

97.681

0.672
  pilT Pseudomonas aeruginosa PAK

66.765

98.551

0.658
  pilT Legionella pneumophila strain Lp02

67.477

95.362

0.643
  pilT Legionella pneumophila strain ERS1305867

67.477

95.362

0.643
  pilT Neisseria meningitidis 8013

64.545

95.652

0.617
  pilT Neisseria gonorrhoeae MS11

64.242

95.652

0.614
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.988

94.783

0.493
  pilU Vibrio cholerae strain A1552

42.216

96.812

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.828

97.971

0.4
  pilU Acinetobacter baylyi ADP1

40.625

92.754

0.377

Multiple sequence alignment