Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GPW70_RS17025 Genome accession   NZ_CP046740
Coordinates   2661102..2662208 (-) Length   368 a.a.
NCBI ID   WP_000422572.1    Uniprot ID   -
Organism   Vibrio cholerae strain 3566-06     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2656102..2667208
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPW70_RS17000 gshB 2656701..2657657 (+) 957 WP_000593313.1 glutathione synthase -
  GPW70_RS17005 - 2657693..2658256 (+) 564 WP_019828858.1 YqgE/AlgH family protein -
  GPW70_RS17010 ruvX 2658337..2658759 (+) 423 WP_019828856.1 Holliday junction resolvase RuvX -
  GPW70_RS17015 tyrS 2658798..2660078 (-) 1281 WP_019828854.1 tyrosine--tRNA ligase -
  GPW70_RS17020 - 2660187..2660984 (-) 798 WP_001123226.1 helix-turn-helix transcriptional regulator -
  GPW70_RS17025 pilU 2661102..2662208 (-) 1107 WP_000422572.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GPW70_RS17030 pilT 2662223..2663260 (-) 1038 WP_000350195.1 type IV pilus twitching motility protein PilT Machinery gene
  GPW70_RS17035 - 2663286..2663996 (+) 711 WP_001888157.1 YggS family pyridoxal phosphate-dependent enzyme -
  GPW70_RS17040 proC 2664049..2664867 (+) 819 WP_000437941.1 pyrroline-5-carboxylate reductase -
  GPW70_RS17045 - 2664921..2665478 (+) 558 WP_001087261.1 YggT family protein -
  GPW70_RS17050 yggU 2665478..2665768 (+) 291 WP_000741914.1 DUF167 family protein YggU -
  GPW70_RS17055 - 2665814..2666245 (+) 432 WP_001233675.1 DUF4426 domain-containing protein -
  GPW70_RS17060 - 2666315..2666917 (+) 603 WP_032472721.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41307.27 Da        Isoelectric Point: 6.5076

>NTDB_id=406934 GPW70_RS17025 WP_000422572.1 2661102..2662208(-) (pilU) [Vibrio cholerae strain 3566-06]
MELNQYLDGMLTHKASDLYITVGAPILYRVDGELRAQGEALSVADVTALLHAMMDDARQAEFKQTREANFAVVRDSGRFR
VSAFFQRELPGAVIRRIETRIPTFEELKLPEVLQNLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNQHRTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQREQFLLDLSLNLKGVIAQQLLRDKNGKGRHGVFEVLLNSPRIADLIRRGELHELKATMARSQEVGMQTFDQALY
QLVVDDKISEQDALHSADSANDLRLMLKTKRGDDYGSGSLQNVKIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=406934 GPW70_RS17025 WP_000422572.1 2661102..2662208(-) (pilU) [Vibrio cholerae strain 3566-06]
ATGGAGTTGAATCAATATCTGGATGGCATGCTGACTCATAAAGCATCGGATCTGTACATTACGGTGGGTGCACCGATTTT
GTATCGAGTCGATGGTGAATTGCGTGCACAAGGTGAAGCGCTCAGCGTGGCGGATGTGACGGCCTTATTGCATGCGATGA
TGGATGACGCAAGGCAAGCAGAATTTAAGCAGACGCGCGAGGCGAATTTTGCTGTGGTGCGTGACAGTGGCCGTTTCCGG
GTGAGTGCATTTTTCCAACGTGAATTGCCGGGGGCGGTGATCCGCCGGATTGAAACGCGCATTCCCACCTTTGAAGAACT
GAAATTGCCTGAAGTGCTGCAAAATTTGGCGATTGCCAAACGCGGTTTAGTGTTGGTGGTCGGCGCAACCGGTTCGGGTA
AATCGACCACTATGGCAGCCATGACGGGCTATCGTAATCAGCATCGCACCGGGCATATTTTGACGGTCGAAGATCCCATT
GAGTTTGTGCATGAACACAAGCGCTGTATCGTGACGCAGCGAGAAGTGGGGCTCGATACCGAAAGTTATGAAGTGGCGCT
GAAAAACTCGCTGCGCCAAGCGCCGGATATGATTTTGATTGGTGAAATTCGCAGCCGAGAAACCATGGAATACGCGATGA
CTTTCGCGGAAACGGGGCATTTGTGTATGGCCACGCTGCACGCTAACAATGCTAACCAAGCGTTAGAGCGGATTTTGCAT
TTAGTGCCGAAAGAGCAGCGTGAGCAGTTTTTGCTCGATTTGTCACTCAACCTCAAAGGCGTGATTGCCCAGCAATTATT
GCGCGATAAAAATGGCAAAGGTCGACACGGGGTGTTTGAAGTGTTGCTCAATAGCCCGCGTATCGCGGATTTAATTCGCC
GCGGTGAACTGCATGAGCTGAAAGCGACCATGGCTCGTTCGCAGGAAGTGGGGATGCAAACCTTTGATCAAGCCTTATAC
CAGCTTGTGGTGGATGACAAAATCAGTGAGCAAGATGCCCTGCACAGTGCCGATTCCGCCAACGATCTGCGTTTGATGCT
CAAAACCAAGCGTGGTGATGACTACGGCAGCGGCTCTTTGCAGAATGTGAAGATTGATATGGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

100

100

1
  pilU Pseudomonas stutzeri DSM 10701

58.857

95.109

0.56
  pilU Acinetobacter baylyi ADP1

54.701

95.38

0.522
  pilT Legionella pneumophila strain Lp02

42.773

92.12

0.394
  pilT Legionella pneumophila strain ERS1305867

42.773

92.12

0.394
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.806

84.239

0.386
  pilT Vibrio cholerae strain A1552

42.216

90.761

0.383
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.216

90.761

0.383
  pilT Acinetobacter baumannii D1279779

41.593

92.12

0.383
  pilT Acinetobacter baumannii strain A118

41.593

92.12

0.383
  pilT Acinetobacter nosocomialis M2

41.593

92.12

0.383
  pilT Pseudomonas aeruginosa PAK

41.298

92.12

0.38
  pilT Pseudomonas stutzeri DSM 10701

41.003

92.12

0.378
  pilT Acinetobacter baylyi ADP1

42.462

88.315

0.375
  pilT Neisseria gonorrhoeae MS11

38.329

94.293

0.361
  pilT Neisseria meningitidis 8013

38.551

93.75

0.361

Multiple sequence alignment