Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GPW74_RS11595 Genome accession   NZ_CP046737
Coordinates   2531041..2532147 (+) Length   368 a.a.
NCBI ID   WP_032478770.1    Uniprot ID   -
Organism   Vibrio cholerae strain 2015V-1126     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2526041..2537147
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPW74_RS11560 - 2526332..2526934 (-) 603 WP_000725008.1 XTP/dITP diphosphatase -
  GPW74_RS11565 - 2527004..2527435 (-) 432 WP_123012645.1 DUF4426 domain-containing protein -
  GPW74_RS11570 yggU 2527481..2527771 (-) 291 WP_000741916.1 DUF167 family protein YggU -
  GPW74_RS11575 - 2527771..2528328 (-) 558 WP_123012646.1 YggT family protein -
  GPW74_RS11580 proC 2528382..2529200 (-) 819 WP_123012647.1 pyrroline-5-carboxylate reductase -
  GPW74_RS11585 - 2529253..2529963 (-) 711 WP_001256653.1 YggS family pyridoxal phosphate-dependent enzyme -
  GPW74_RS11590 pilT 2529989..2531026 (+) 1038 WP_123012648.1 type IV pilus twitching motility protein PilT Machinery gene
  GPW74_RS11595 pilU 2531041..2532147 (+) 1107 WP_032478770.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GPW74_RS11600 - 2532265..2533062 (+) 798 WP_175246223.1 helix-turn-helix transcriptional regulator -
  GPW74_RS11605 tyrS 2533171..2534451 (+) 1281 WP_142735254.1 tyrosine--tRNA ligase -
  GPW74_RS11610 ruvX 2534489..2534911 (-) 423 WP_000091868.1 Holliday junction resolvase RuvX -
  GPW74_RS11615 - 2534992..2535555 (-) 564 WP_123012649.1 YqgE/AlgH family protein -
  GPW74_RS11620 gshB 2535591..2536547 (-) 957 WP_111674000.1 glutathione synthase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41337.30 Da        Isoelectric Point: 6.5076

>NTDB_id=406878 GPW74_RS11595 WP_032478770.1 2531041..2532147(+) (pilU) [Vibrio cholerae strain 2015V-1126]
MELNQYLDGMLTHKASDLYITVGAPILYRVDGELRAQGEALSVADVTALLHAMMDDARQAEFKQTREANFAVVRDSGRFR
VSAFFQRELPGAVIRRIETRIPTFEELKLPEVLQNLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNQHRTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQREQFLLDLSLNLKGVIAQQLLRDKNGKGRHGVFEILLNSPRISDLIRRGELHELKATMARSQEVGMQTFDQALY
QLVVDDKISEQDALHSADSANDLRLMLKTKRGDDYGSGSLQNVKIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=406878 GPW74_RS11595 WP_032478770.1 2531041..2532147(+) (pilU) [Vibrio cholerae strain 2015V-1126]
ATGGAGTTGAATCAATATCTGGATGGCATGCTGACTCATAAAGCATCGGATCTGTACATTACGGTGGGTGCACCGATTTT
GTATCGAGTCGATGGTGAATTGCGTGCACAAGGTGAAGCGCTCAGCGTGGCGGATGTGACGGCCTTATTGCATGCGATGA
TGGATGACGCAAGGCAAGCAGAATTTAAGCAGACGCGCGAAGCGAACTTTGCTGTGGTGCGTGACAGTGGCCGTTTTCGG
GTCAGTGCATTTTTCCAACGAGAATTGCCGGGGGCGGTGATCCGCCGGATTGAAACGCGCATTCCCACCTTTGAAGAACT
CAAATTGCCTGAAGTGCTGCAAAATTTGGCGATTGCCAAACGCGGTTTAGTGTTGGTGGTTGGCGCAACCGGTTCGGGTA
AATCGACCACTATGGCAGCCATGACTGGCTATCGTAATCAGCATCGCACCGGGCATATTTTGACGGTCGAAGATCCCATT
GAGTTTGTGCATGAACACAAGCGCTGTATCGTGACGCAGCGAGAAGTGGGGCTCGATACCGAAAGTTATGAAGTGGCGCT
GAAAAACTCGCTGCGCCAAGCCCCCGATATGATTTTGATTGGCGAAATTCGCAGCCGAGAAACCATGGAATACGCGATGA
CTTTCGCGGAAACCGGGCATTTGTGTATGGCCACTTTGCACGCCAATAACGCCAACCAAGCATTAGAGCGGATTTTGCAT
CTGGTGCCCAAAGAGCAGCGTGAGCAGTTTTTGCTCGATTTGTCACTCAACCTCAAAGGCGTGATTGCCCAGCAATTATT
GCGCGATAAAAATGGCAAAGGTCGACACGGGGTGTTTGAAATTCTGCTCAATAGCCCGCGCATTTCCGATTTGATCCGCC
GTGGCGAGCTACACGAGCTAAAAGCGACCATGGCTCGTTCGCAGGAAGTGGGGATGCAAACTTTCGATCAAGCCTTATAC
CAGCTTGTGGTGGATGACAAAATCAGTGAGCAAGATGCCCTGCACAGTGCCGATTCAGCCAACGATCTGCGTTTGATGCT
CAAAACCAAGCGTGGTGATGACTACGGCAGCGGCTCTTTGCAGAATGTGAAGATTGATATGGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

99.457

100

0.995
  pilU Pseudomonas stutzeri DSM 10701

58.286

95.109

0.554
  pilU Acinetobacter baylyi ADP1

54.701

95.38

0.522
  pilT Legionella pneumophila strain Lp02

43.068

92.12

0.397
  pilT Legionella pneumophila strain ERS1305867

43.068

92.12

0.397
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.129

84.239

0.389
  pilT Acinetobacter baumannii D1279779

41.888

92.12

0.386
  pilT Acinetobacter baumannii strain A118

41.888

92.12

0.386
  pilT Acinetobacter nosocomialis M2

41.888

92.12

0.386
  pilT Vibrio cholerae strain A1552

42.515

90.761

0.386
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.515

90.761

0.386
  pilT Pseudomonas aeruginosa PAK

41.593

92.12

0.383
  pilT Pseudomonas stutzeri DSM 10701

41.298

92.12

0.38
  pilT Acinetobacter baylyi ADP1

42.769

88.315

0.378
  pilT Neisseria meningitidis 8013

38.841

93.75

0.364
  pilT Neisseria gonorrhoeae MS11

38.617

94.293

0.364

Multiple sequence alignment