Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GPW74_RS11590 Genome accession   NZ_CP046737
Coordinates   2529989..2531026 (+) Length   345 a.a.
NCBI ID   WP_123012648.1    Uniprot ID   -
Organism   Vibrio cholerae strain 2015V-1126     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2524989..2536026
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPW74_RS11555 hemW 2525163..2526338 (-) 1176 WP_123012644.1 radical SAM family heme chaperone HemW -
  GPW74_RS11560 - 2526332..2526934 (-) 603 WP_000725008.1 XTP/dITP diphosphatase -
  GPW74_RS11565 - 2527004..2527435 (-) 432 WP_123012645.1 DUF4426 domain-containing protein -
  GPW74_RS11570 yggU 2527481..2527771 (-) 291 WP_000741916.1 DUF167 family protein YggU -
  GPW74_RS11575 - 2527771..2528328 (-) 558 WP_123012646.1 YggT family protein -
  GPW74_RS11580 proC 2528382..2529200 (-) 819 WP_123012647.1 pyrroline-5-carboxylate reductase -
  GPW74_RS11585 - 2529253..2529963 (-) 711 WP_001256653.1 YggS family pyridoxal phosphate-dependent enzyme -
  GPW74_RS11590 pilT 2529989..2531026 (+) 1038 WP_123012648.1 type IV pilus twitching motility protein PilT Machinery gene
  GPW74_RS11595 pilU 2531041..2532147 (+) 1107 WP_032478770.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GPW74_RS11600 - 2532265..2533062 (+) 798 WP_175246223.1 helix-turn-helix transcriptional regulator -
  GPW74_RS11605 tyrS 2533171..2534451 (+) 1281 WP_142735254.1 tyrosine--tRNA ligase -
  GPW74_RS11610 ruvX 2534489..2534911 (-) 423 WP_000091868.1 Holliday junction resolvase RuvX -
  GPW74_RS11615 - 2534992..2535555 (-) 564 WP_123012649.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38261.85 Da        Isoelectric Point: 6.6672

>NTDB_id=406877 GPW74_RS11590 WP_123012648.1 2529989..2531026(+) (pilT) [Vibrio cholerae strain 2015V-1126]
MDIAELLEFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFTHSDVHRLIFEIMNDAQRSEYEEKLEVDFSFELPNVGR
FRVNAFHQARGCSAVFRTIPTVIPTLEQLDAPEIFSKIANYEKGLVLVTGPTGSGKSTTLAAMVNYVNAHHNKHILTIED
PIEFVHSNNKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRVGGGRVACHEIMLATPAIRNLIREDKVAQMYSIIQTGATHGMQTMEQNA
KQLIARGVVDAQEVQSKIELDLKAF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=406877 GPW74_RS11590 WP_123012648.1 2529989..2531026(+) (pilT) [Vibrio cholerae strain 2015V-1126]
ATGGATATCGCTGAGTTACTGGAATTTAGTGTAAAACATAACGCCTCAGATCTACATCTTTCCGCAGGTGTTCCGCCTAT
GGTACGGATTGATGGCGAAGTTAGGAAGCTTGGCGTGCCTGCTTTTACCCATTCTGATGTACATCGCTTGATTTTTGAGA
TCATGAATGATGCCCAGCGCAGTGAATATGAAGAGAAATTAGAAGTCGATTTTTCTTTTGAACTGCCTAATGTTGGCCGT
TTCCGGGTTAACGCGTTTCATCAAGCGCGCGGATGCTCGGCGGTGTTTCGTACCATTCCCACTGTCATCCCAACGTTAGA
GCAACTGGATGCTCCTGAGATCTTCAGCAAAATTGCCAATTATGAAAAAGGGTTAGTTTTAGTGACTGGGCCAACGGGGT
CGGGTAAGTCGACGACCTTGGCGGCGATGGTGAACTATGTCAATGCCCATCACAATAAGCATATTTTGACTATTGAAGAT
CCGATTGAATTTGTGCACAGCAACAATAAGTGTTTGATCAACCAACGCGAAGTGCACCGTGATACCCACAGTTTTAAAAA
TGCATTGCGCTCGGCATTGCGTGAAGACCCAGATGTGATTTTGGTTGGTGAGCTGCGTGACCAAGAAACGATTAGCTTGG
CGCTTACCGCGGCAGAAACTGGTCACTTGGTGTTTGGCACTCTACACACCAGCTCGGCGGCGAAAACCATTGACCGGATT
ATCGATGTCTTTCCGGGCAGCGACAAAGACATGGTGCGTTCCATGCTGTCGGAATCGCTGCGTGCGGTTATTGCGCAAAA
ACTCCTAAAACGCGTCGGGGGTGGCCGTGTCGCGTGTCATGAAATCATGCTGGCGACGCCGGCAATTCGGAACTTGATCC
GTGAAGATAAAGTGGCGCAGATGTATTCGATCATCCAAACCGGTGCCACACACGGCATGCAAACCATGGAGCAAAACGCC
AAACAGCTGATCGCTCGTGGTGTGGTGGATGCGCAAGAAGTACAGAGCAAAATCGAGTTGGATTTAAAAGCGTTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

99.71

100

0.997
  pilT Vibrio cholerae strain A1552

99.71

100

0.997
  pilT Acinetobacter baumannii D1279779

71.726

97.391

0.699
  pilT Acinetobacter baumannii strain A118

71.726

97.391

0.699
  pilT Acinetobacter nosocomialis M2

71.429

97.391

0.696
  pilT Acinetobacter baylyi ADP1

72.727

95.652

0.696
  pilT Pseudomonas stutzeri DSM 10701

68.843

97.681

0.672
  pilT Pseudomonas aeruginosa PAK

66.765

98.551

0.658
  pilT Legionella pneumophila strain Lp02

67.173

95.362

0.641
  pilT Legionella pneumophila strain ERS1305867

67.173

95.362

0.641
  pilT Neisseria meningitidis 8013

64.242

95.652

0.614
  pilT Neisseria gonorrhoeae MS11

63.939

95.652

0.612
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.988

94.783

0.493
  pilU Vibrio cholerae strain A1552

42.216

96.812

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.828

97.971

0.4
  pilU Acinetobacter baylyi ADP1

40.625

92.754

0.377

Multiple sequence alignment