Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GO600_RS11170 Genome accession   NZ_CP046617
Coordinates   2091760..2092848 (+) Length   362 a.a.
NCBI ID   WP_028494590.1    Uniprot ID   -
Organism   Thermus antranikianii strain JCM 19900     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2086760..2097848
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GO600_RS11145 (GO600_11100) ribH 2086896..2087369 (+) 474 WP_028494595.1 6,7-dimethyl-8-ribityllumazine synthase -
  GO600_RS11150 (GO600_11105) - 2087356..2087760 (-) 405 WP_028494594.1 DUF4395 family protein -
  GO600_RS11155 (GO600_11110) pgeF 2087840..2088565 (+) 726 WP_028494593.1 peptidoglycan editing factor PgeF -
  GO600_RS11160 (GO600_11115) - 2088600..2089082 (+) 483 WP_028494592.1 YqeG family HAD IIIA-type phosphatase -
  GO600_RS11165 (GO600_11120) pilF 2089079..2091748 (+) 2670 WP_028494591.1 type IV pilus assembly ATPase PilB Machinery gene
  GO600_RS11170 (GO600_11125) pilT 2091760..2092848 (+) 1089 WP_028494590.1 type IV pilus twitching motility protein PilT Machinery gene
  GO600_RS11175 (GO600_11130) gatB 2092879..2094288 (+) 1410 WP_028494589.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB -
  GO600_RS11180 (GO600_11135) purM 2094294..2095295 (+) 1002 WP_028494588.1 phosphoribosylformylglycinamidine cyclo-ligase -
  GO600_RS11185 (GO600_11140) - 2095292..2095921 (+) 630 WP_028494587.1 histidine phosphatase family protein -

Sequence

Protein


Download         Length: 362 a.a.        Molecular weight: 40114.45 Da        Isoelectric Point: 7.2367

>NTDB_id=406072 GO600_RS11170 WP_028494590.1 2091760..2092848(+) (pilT) [Thermus antranikianii strain JCM 19900]
MAKTPDIVDLLTLAVERGASDLVITVGLPPMIKVDGEFHPTEYEPLSPQDTRRLMYALMDEKQQRIFEEEKELDFSFSLP
GKGRYRVNVFLQRGSVGGVLRVVPATIKSFEELGLPKTIAEIAMAPRGLVLVTGPTGSGKSTTLASMIDYINERKPVHIV
TIEDPIEFFHKHKKAIVNQREIGSDTHSFHKALRSVLRQAPDVILVGEMRDYETIAAAITAAETGHLVMGTLHTNSAPET
IDRIIDVFPENQQEQVRVQLSNNLVAVLTQQLLPKAFGGGRVLAYELMIATPAVRALIREGKSHQLRSVIQTGGQYGMIT
MDACLADLYKRKLITYEMGLSRAVDPKEFMRLAGVQEGSKRP

Nucleotide


Download         Length: 1089 bp        

>NTDB_id=406072 GO600_RS11170 WP_028494590.1 2091760..2092848(+) (pilT) [Thermus antranikianii strain JCM 19900]
ATGGCGAAAACACCGGACATCGTCGATCTTCTGACCCTGGCTGTAGAGCGAGGGGCCAGCGATTTGGTGATCACCGTGGG
CCTTCCCCCCATGATCAAGGTGGACGGAGAGTTCCACCCCACGGAGTACGAGCCCCTTTCTCCCCAGGATACCCGCCGGC
TCATGTACGCCCTCATGGACGAAAAGCAGCAGCGGATCTTTGAAGAGGAAAAGGAGCTGGACTTCTCCTTCAGCCTCCCC
GGAAAAGGGCGGTACCGGGTAAACGTTTTCCTCCAACGGGGAAGCGTGGGGGGGGTCCTAAGGGTGGTGCCCGCCACCAT
CAAGAGCTTTGAGGAACTCGGCTTGCCCAAGACCATCGCCGAGATCGCCATGGCTCCCAGGGGCCTGGTCCTGGTCACGG
GGCCCACTGGGTCGGGGAAGAGCACCACCTTAGCCTCCATGATCGACTACATCAACGAACGCAAGCCCGTGCACATCGTG
ACCATTGAGGACCCCATAGAGTTCTTCCACAAGCACAAAAAGGCCATCGTCAACCAGCGGGAGATCGGCTCCGATACCCA
CAGCTTCCACAAGGCGCTTAGAAGCGTTCTCCGCCAGGCCCCGGATGTGATCCTGGTGGGGGAGATGCGGGACTACGAGA
CCATCGCCGCCGCCATCACCGCCGCGGAAACCGGGCATTTGGTCATGGGCACCCTGCACACCAACTCTGCCCCCGAGACC
ATCGACCGCATCATCGACGTCTTCCCGGAAAACCAGCAGGAGCAAGTGCGGGTGCAGCTTTCCAACAACCTGGTGGCGGT
GCTCACCCAGCAGCTTCTGCCCAAGGCCTTCGGGGGCGGAAGGGTCTTGGCCTACGAGCTCATGATCGCCACCCCTGCGG
TAAGGGCCTTGATCCGGGAGGGCAAAAGCCACCAGCTCCGGAGCGTCATCCAGACCGGGGGCCAGTACGGGATGATCACC
ATGGATGCCTGCCTGGCGGATCTCTACAAGCGCAAGCTGATCACCTACGAGATGGGCTTGAGCCGGGCGGTGGACCCCAA
GGAGTTCATGCGCCTGGCCGGGGTGCAGGAGGGGAGCAAGCGCCCTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

68.839

97.514

0.671
  pilT Pseudomonas aeruginosa PAK

54.07

95.028

0.514
  pilT Acinetobacter nosocomialis M2

53.913

95.304

0.514
  pilT Acinetobacter baumannii strain A118

53.913

95.304

0.514
  pilT Acinetobacter baumannii D1279779

53.913

95.304

0.514
  pilT Pseudomonas stutzeri DSM 10701

53.779

95.028

0.511
  pilT Acinetobacter baylyi ADP1

53.779

95.028

0.511
  pilT Legionella pneumophila strain Lp02

52.035

95.028

0.494
  pilT Legionella pneumophila strain ERS1305867

52.035

95.028

0.494
  pilT Neisseria meningitidis 8013

52.663

93.37

0.492
  pilT Neisseria gonorrhoeae MS11

51.929

93.094

0.483
  pilT Vibrio cholerae strain A1552

52.888

90.884

0.481
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.888

90.884

0.481
  pilU Pseudomonas stutzeri DSM 10701

47.813

94.751

0.453
  pilU Vibrio cholerae strain A1552

45.21

92.265

0.417
  pilU Acinetobacter baylyi ADP1

41.341

98.895

0.409

Multiple sequence alignment