Detailed information NTG0406071

Bioinformatically predicted

Overview

• Name: pilF
• Type: Machinery gene
• Locus tag: GO600_RS11165
• Genome accession: NZ_CP046617
• Coordinates: 2089079..2091748 (+)
• Length: 889 a.a.
• NCBI ID: WP_028494591.1
• Uniprot ID: -
• Organism: Thermus antranikianii strain JCM 19900
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2084079..2096748

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GO600_RS11120 (GO600_11075)	-	2084457..2085260 (+)	804	WP_028494600.1	histidinol-phosphatase HisJ family protein	-
GO600_RS11125 (GO600_11080)	-	2085236..2085505 (-)	270	WP_028494599.1	hypothetical protein	-
GO600_RS11130 (GO600_11085)	-	2085502..2086116 (-)	615	WP_028494598.1	sulfite oxidase-like oxidoreductase	-
GO600_RS11135 (GO600_11090)	-	2086161..2086493 (-)	333	WP_028494597.1	DUF190 domain-containing protein	-
GO600_RS11140 (GO600_11095)	crcB	2086498..2086875 (-)	378	WP_028494596.1	fluoride efflux transporter CrcB	-
GO600_RS11145 (GO600_11100)	ribH	2086896..2087369 (+)	474	WP_028494595.1	6,7-dimethyl-8-ribityllumazine synthase	-
GO600_RS11150 (GO600_11105)	-	2087356..2087760 (-)	405	WP_028494594.1	DUF4395 family protein	-
GO600_RS11155 (GO600_11110)	pgeF	2087840..2088565 (+)	726	WP_028494593.1	peptidoglycan editing factor PgeF	-
GO600_RS11160 (GO600_11115)	-	2088600..2089082 (+)	483	WP_028494592.1	YqeG family HAD IIIA-type phosphatase	-
GO600_RS11165 (GO600_11120)	pilF	2089079..2091748 (+)	2670	WP_028494591.1	type IV pilus assembly ATPase PilB	Machinery gene
GO600_RS11170 (GO600_11125)	pilT	2091760..2092848 (+)	1089	WP_028494590.1	type IV pilus twitching motility protein PilT	Machinery gene
GO600_RS11175 (GO600_11130)	gatB	2092879..2094288 (+)	1410	WP_028494589.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB	-
GO600_RS11180 (GO600_11135)	purM	2094294..2095295 (+)	1002	WP_028494588.1	phosphoribosylformylglycinamidine cyclo-ligase	-
GO600_RS11185 (GO600_11140)	-	2095292..2095921 (+)	630	WP_028494587.1	histidine phosphatase family protein	-

Sequence

Protein

• Length: 889 a.a.
• Molecular weight: 98107.16 Da
• Isoelectric Point: 5.0554

>NTDB_id=406071 GO600_RS11165 WP_028494591.1 2089079..2091748(+) (pilF) [Thermus antranikianii strain JCM 19900]
MSVLTIGDKRLGAILLDAGLLTDEELQMALEKHREVGGSLAEVIVDSGLLSERRIAQAIEDHFGIPLVELHTLEIPPKVK
ALLPAEKAKELQAIPFALDEEAGVVRVAFVNPLDTLALEEVEDLTGMVVEPYQATKSAFLYALAKHYPELNLPLPPPPSG
PSREELRVGELLVEKGLLDRNTLEEALVEQEKTGDLLGRILVRKGLPEEVLYRVLAEQKGMEFLPSTQGLSPDPAATNLL
LRSDALRYSAVPVGFNNGEVEVVLADPRHKEAVAELLGRPARFFLTLPKEWEALFHQAYPEKSRLGEVLVQEGRLSREDL
REALEVQRRLPKAKPLGEILVELGLARPEDVEEALKKQRQGGGRLEDTLVASGKLRPEALAQAVATQLGYAYINPEENPP
DPGAALLLPEDLARRYGIFPHRLEGKTLVLLMKDPRNILALDDVRLALKRKGLAYEVQPAVATEAAITKLIERFYGKEEL
GEIAKELSKGYQEEEEAVTPELDESAAQKFVKQVIREAFLQDASDIHIEPRQSDVLVRLRIDGVLRQYTTLPKGALNSII
SVVKIMGGLNIAERRLPQDGRVRYREGSIDVDLRLSTLPTVYGEKAVMRLLKKAADIPEIEGLGFAPGVFERFQEVISKP
YGIFLITGPTGSGKSFTTFSILKRIATPDKNTQTIEDPVEYEIPGINQTQVNPQAGLTFARALRAFLRQDPDIIMVGEIR
DSETAKIATEAALTGHLVIATLHTNDAAQAITRLDEMGVELFNISAALIGVLSQRLVRKICDHCKVEVKPDPEVLRRLGL
SEEEIQGAKLYKGMGCERCGGTGYKGRYAIHELLVVDDEIRHAIVAGKSATEIKEIARKKGMKTLREDGIYKALQGITTL
EEVLARTIE

Nucleotide

• Length: 2670 bp

>NTDB_id=406071 GO600_RS11165 WP_028494591.1 2089079..2091748(+) (pilF) [Thermus antranikianii strain JCM 19900]
ATGAGCGTGCTCACCATCGGGGACAAACGGCTAGGTGCCATCCTATTGGATGCCGGCCTCCTCACCGACGAGGAGCTGCA
GATGGCCCTGGAGAAGCACCGGGAGGTGGGGGGTTCCTTGGCCGAGGTCATCGTGGACTCGGGGCTGCTTTCGGAAAGGC
GGATCGCCCAGGCCATCGAGGACCACTTCGGCATCCCCCTGGTGGAGCTCCACACTTTGGAGATCCCCCCCAAGGTGAAG
GCCCTCCTGCCGGCGGAGAAGGCCAAGGAACTCCAGGCCATCCCCTTCGCCCTGGACGAGGAGGCAGGGGTGGTGCGGGT
GGCCTTCGTAAACCCCCTGGACACCTTGGCCCTCGAGGAGGTGGAGGACCTCACCGGGATGGTGGTGGAACCCTACCAGG
CCACCAAGAGCGCCTTCCTCTACGCCTTGGCCAAGCACTACCCCGAGCTCAACCTGCCCCTACCCCCTCCCCCCAGCGGC
CCCAGCCGGGAGGAGCTGAGGGTGGGCGAGCTTCTGGTGGAAAAGGGCCTCCTGGACCGGAACACCCTGGAGGAAGCCCT
GGTGGAGCAGGAAAAGACCGGGGATCTCCTGGGCCGCATCCTGGTGCGAAAGGGGCTACCCGAGGAAGTCCTCTACCGGG
TTTTGGCCGAGCAGAAGGGAATGGAATTTCTCCCCTCCACCCAGGGCCTATCCCCCGATCCGGCCGCCACCAACCTTCTC
CTGCGCTCGGATGCCCTGCGCTATAGCGCCGTGCCCGTGGGCTTCAACAACGGGGAGGTGGAGGTGGTCCTCGCCGATCC
CCGTCACAAGGAGGCAGTGGCGGAGCTCCTTGGGCGCCCCGCCCGCTTCTTCCTCACCCTTCCCAAGGAGTGGGAAGCCC
TTTTCCACCAGGCCTATCCGGAAAAAAGCCGCCTGGGGGAGGTCCTGGTCCAGGAGGGGCGCCTGAGCCGGGAGGATTTA
AGAGAGGCCCTCGAGGTGCAAAGGCGTCTGCCCAAGGCCAAGCCCCTGGGGGAGATCCTGGTGGAGCTGGGCTTGGCCCG
GCCCGAGGACGTGGAGGAAGCCCTGAAGAAGCAACGCCAGGGTGGAGGCCGCCTCGAGGACACCCTGGTGGCCTCGGGCA
AGCTCAGGCCCGAAGCCCTGGCCCAGGCGGTGGCCACCCAGCTGGGCTACGCCTACATCAACCCCGAGGAGAACCCTCCG
GATCCCGGGGCCGCCCTCCTCCTCCCCGAGGACCTGGCCCGGCGGTACGGGATCTTCCCCCACCGCCTGGAGGGCAAGAC
CCTGGTCCTCCTCATGAAGGATCCCAGGAACATCCTGGCCCTGGACGACGTGCGCTTGGCCCTTAAGCGCAAGGGCCTGG
CCTACGAGGTTCAGCCCGCCGTGGCCACGGAGGCCGCCATCACCAAGCTCATCGAACGCTTCTACGGCAAGGAGGAACTC
GGCGAGATCGCCAAGGAGCTGTCCAAGGGCTACCAGGAGGAGGAGGAAGCCGTCACCCCTGAGCTGGACGAAAGCGCCGC
CCAGAAGTTTGTCAAACAGGTAATCCGGGAGGCCTTCCTGCAGGATGCCTCCGACATCCACATAGAACCCCGCCAGTCCG
ACGTCCTGGTGCGCCTTCGCATCGACGGGGTCTTGCGCCAGTACACCACCCTGCCCAAGGGGGCCCTAAACTCCATCATC
AGCGTGGTCAAGATCATGGGCGGCCTCAACATCGCCGAAAGGCGCCTGCCCCAGGACGGGCGGGTGCGCTACCGGGAAGG
TTCCATCGACGTGGACCTCCGCCTCTCCACCCTGCCCACGGTCTACGGGGAAAAGGCGGTGATGCGCCTTCTGAAAAAGG
CCGCCGACATCCCCGAGATCGAGGGCCTGGGCTTCGCCCCTGGGGTCTTTGAGCGCTTCCAGGAGGTGATCTCCAAGCCC
TACGGCATCTTCCTCATCACCGGGCCCACCGGAAGCGGCAAAAGCTTCACCACCTTCTCCATCCTCAAGCGCATCGCCAC
CCCCGACAAGAACACCCAGACCATCGAGGACCCCGTGGAGTACGAGATCCCCGGCATCAACCAGACCCAGGTGAACCCCC
AAGCTGGCCTCACCTTTGCCCGGGCCCTCAGGGCCTTCCTAAGGCAGGACCCGGACATCATCATGGTGGGGGAGATCCGG
GACTCGGAAACCGCCAAGATCGCCACCGAGGCCGCTCTCACCGGCCACCTGGTCATCGCCACCCTGCACACCAACGACGC
CGCCCAGGCCATCACCCGCCTGGACGAGATGGGGGTGGAGCTTTTCAACATCTCCGCCGCCCTCATCGGGGTTCTCTCCC
AGCGTCTGGTGCGGAAGATCTGCGACCACTGCAAGGTAGAGGTGAAGCCCGACCCCGAGGTCCTGCGGCGCTTAGGCCTT
TCCGAAGAGGAGATCCAAGGGGCCAAGCTCTACAAGGGGATGGGATGCGAGCGCTGCGGGGGAACGGGCTATAAGGGGCG
CTACGCCATCCACGAGCTTTTGGTGGTGGACGACGAGATCCGCCACGCCATCGTGGCCGGCAAGTCGGCCACGGAGATCA
AGGAGATCGCCAGGAAAAAGGGCATGAAAACCCTGCGGGAGGACGGCATCTACAAGGCCCTCCAGGGAATCACCACCCTC
GAGGAGGTCCTGGCGCGTACCATTGAATAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (173-303)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (45-148)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (369-479)

• Type II/IV secretion system protein (T2SSE) (513-779)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilF	Thermus thermophilus HB27	87.852	100	0.879
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	56.517	100	0.566