Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GOY17_RS14770 Genome accession   NZ_CP046603
Coordinates   3230365..3231402 (-) Length   345 a.a.
NCBI ID   WP_115842568.1    Uniprot ID   A0A3D8VCQ5
Organism   Lysobacter soli strain XL170     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3225365..3236402
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GOY17_RS14760 (GOY17_14760) - 3226707..3228980 (+) 2274 WP_198173703.1 ATP-binding protein -
  GOY17_RS14765 (GOY17_14765) pilU 3229063..3230235 (-) 1173 WP_115842567.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GOY17_RS14770 (GOY17_14770) pilT 3230365..3231402 (-) 1038 WP_115842568.1 type IV pilus twitching motility protein PilT Machinery gene
  GOY17_RS14775 (GOY17_14775) - 3231513..3232196 (+) 684 WP_232338443.1 YggS family pyridoxal phosphate-dependent enzyme -
  GOY17_RS14780 (GOY17_14780) proC 3232233..3233069 (+) 837 WP_157030967.1 pyrroline-5-carboxylate reductase -
  GOY17_RS14785 (GOY17_14785) - 3233082..3233594 (+) 513 WP_157030968.1 DUF4426 domain-containing protein -
  GOY17_RS14790 (GOY17_14790) - 3233821..3234621 (-) 801 WP_157030969.1 cobalamin-binding protein -
  GOY17_RS14795 (GOY17_14795) aac(6') 3234618..3235076 (-) 459 WP_157030970.1 aminoglycoside 6'-N-acetyltransferase -
  GOY17_RS14800 (GOY17_14800) pdxH 3235117..3235719 (-) 603 WP_157030971.1 pyridoxamine 5'-phosphate oxidase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38561.34 Da        Isoelectric Point: 6.7269

>NTDB_id=405999 GOY17_RS14770 WP_115842568.1 3230365..3231402(-) (pilT) [Lysobacter soli strain XL170]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPGLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLDDLGCPPIFRELIQQPQGLILVTGPTGSGKSTTLAAMIDHINKNEYGHILSVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRI
IDVFPAGEKPMVRSMLSESLRAVISQSLLKKVGGGRTAAWEIMVGIPAIRNLIREDKVAQMYSAIQTGQNYGMMTLDQHL
QDLVKRGLILRPQAKEYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=405999 GOY17_RS14770 WP_115842568.1 3230365..3231402(-) (pilT) [Lysobacter soli strain XL170]
ATGGATATCGCCGAACTTCTGGCGTTCTCGGTCAAGAACAAAGCATCCGACCTGCACCTGTCCGCCGGCCTGCCGCCGAT
GATCCGCGTGGACGGCGACGTTCGCCGCATCAACATCCCGGCGCTGGACCACAAGCAGGTCCACGCGCTGGTGTACGACA
TCATGTCGGACAAGCAGCGCCGCGATTACGAGGAATTCCTCGAAGTCGACTTCTCGTTCGAGATTCCCGGCCTGGCGCGC
TTCCGCGTCAACGCGTTCAACCAGAACCGCGGCGCCGGTGCGGTGTTCCGTACGATTCCGTCGGAAGTGCTCACGCTCGA
CGACCTGGGTTGCCCGCCGATCTTCCGCGAGCTGATCCAGCAGCCGCAGGGCCTGATCCTGGTGACCGGTCCGACCGGCT
CGGGCAAGTCGACCACGCTCGCGGCGATGATCGACCACATCAACAAGAACGAATACGGCCACATCCTCTCCGTCGAGGAT
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAGGTGCACCGCGACACGCACGGCTTCAACGA
GGCGCTACGCTCGGCGCTGCGCGAAGACCCCGACTACATCCTCGTCGGCGAATTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACGGCCGCGGAAACCGGCCACCTGGTGTTCGGCACGCTGCACACCTCCTCGGCGGCCAAGACCGTGGACCGAATC
ATCGACGTGTTCCCCGCCGGCGAGAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGCGCGGTGATCTCGCAGTC
GCTGCTGAAGAAGGTCGGCGGCGGTCGTACCGCGGCGTGGGAAATCATGGTCGGCATCCCGGCCATCCGTAACCTGATCC
GCGAAGACAAGGTCGCGCAGATGTACTCGGCGATCCAGACCGGCCAGAACTACGGAATGATGACGCTCGACCAGCACCTG
CAGGACCTCGTCAAGCGCGGCCTGATCCTGCGTCCGCAGGCGAAGGAATACGCGAAGGACAAGCGGTTGTTCGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3D8VCQ5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

79.36

99.71

0.791
  pilT Acinetobacter baumannii strain A118

79.36

99.71

0.791
  pilT Acinetobacter nosocomialis M2

79.36

99.71

0.791
  pilT Pseudomonas stutzeri DSM 10701

77.907

99.71

0.777
  pilT Acinetobacter baylyi ADP1

77.616

99.71

0.774
  pilT Pseudomonas aeruginosa PAK

77.581

98.261

0.762
  pilT Legionella pneumophila strain Lp02

74.128

99.71

0.739
  pilT Legionella pneumophila strain ERS1305867

74.128

99.71

0.739
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.909

95.362

0.667
  pilT Vibrio cholerae strain A1552

69.909

95.362

0.667
  pilT Neisseria meningitidis 8013

65.396

98.841

0.646
  pilT Neisseria gonorrhoeae MS11

64.912

99.13

0.643
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.275

100

0.493
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397
  pilU Pseudomonas stutzeri DSM 10701

40.909

95.652

0.391
  pilU Vibrio cholerae strain A1552

40.308

94.203

0.38

Multiple sequence alignment