Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GNH96_RS07685 Genome accession   NZ_CP046565
Coordinates   1588784..1589818 (-) Length   344 a.a.
NCBI ID   WP_169603141.1    Uniprot ID   A0A858Q7Q7
Organism   Methylococcus geothermalis strain IM1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1583784..1594818
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GNH96_RS07660 (GNH96_07630) - 1584327..1585109 (+) 783 WP_169603136.1 succinate dehydrogenase iron-sulfur subunit -
  GNH96_RS07665 (GNH96_07635) - 1585106..1585363 (+) 258 WP_169603137.1 succinate dehydrogenase assembly factor 2 -
  GNH96_RS07670 (GNH96_07640) - 1585357..1585779 (+) 423 WP_169603138.1 hypothetical protein -
  GNH96_RS07675 (GNH96_07645) - 1585790..1587580 (+) 1791 WP_169603139.1 VWA domain-containing protein -
  GNH96_RS07680 (GNH96_07650) pilU 1587639..1588769 (-) 1131 WP_169603140.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GNH96_RS07685 (GNH96_07655) pilT 1588784..1589818 (-) 1035 WP_169603141.1 type IV pilus twitching motility protein PilT Machinery gene
  GNH96_RS07690 (GNH96_07660) - 1589872..1590561 (+) 690 WP_169603142.1 YggS family pyridoxal phosphate-dependent enzyme -
  GNH96_RS07695 (GNH96_07665) proC 1590629..1591456 (+) 828 WP_169603143.1 pyrroline-5-carboxylate reductase -
  GNH96_RS07700 (GNH96_07670) - 1591456..1592034 (+) 579 WP_169603144.1 YggT family protein -
  GNH96_RS07705 (GNH96_07675) - 1592163..1592531 (+) 369 WP_228720067.1 EAL domain-containing protein -
  GNH96_RS07710 (GNH96_07680) trmB 1592516..1593208 (-) 693 WP_169603146.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
  GNH96_RS07715 (GNH96_07685) - 1593296..1593511 (-) 216 WP_169603147.1 DUF2905 domain-containing protein -
  GNH96_RS07720 (GNH96_07690) - 1593498..1594424 (-) 927 WP_169603148.1 vWA domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38289.98 Da        Isoelectric Point: 6.7754

>NTDB_id=405582 GNH96_RS07685 WP_169603141.1 1588784..1589818(-) (pilT) [Methylococcus geothermalis strain IM1]
MDIAELLAFSVKNKSSDLHLSSGLPPMIRVDGDIRRINVPPLEHKEVHALIYDIMNDKQRRDFEEFLETDFSFELPGVAR
FRVNAFNQDRGAAAVFRTIPSKVLTLEELGCPKFFQDVTRQPRGLIVVTGPTGSGKSTTLAAMIDYINSNDYSHILTIED
PIEFVHQSKKCLINQREVHRNTLGFNEALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAAEKEMIRSMLSESLQAVISQALVKKAGGGRTAAWEIMVGTPAIRNLIREAKVAQMYSAIQTGRKDGMQTLDQHL
QELVEKGVINRQVAREIAVNKAAF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=405582 GNH96_RS07685 WP_169603141.1 1588784..1589818(-) (pilT) [Methylococcus geothermalis strain IM1]
ATGGATATCGCCGAACTGCTCGCCTTCTCGGTCAAGAACAAATCCTCCGACCTGCATCTATCATCCGGACTGCCCCCGAT
GATCCGGGTCGACGGGGATATCCGGCGCATCAACGTGCCGCCGCTGGAACACAAGGAAGTCCACGCACTGATCTACGACA
TCATGAACGACAAGCAGCGGCGCGATTTCGAGGAATTCCTGGAAACGGATTTCTCGTTCGAACTGCCCGGCGTCGCGCGT
TTCCGCGTCAACGCGTTCAACCAGGACCGGGGCGCGGCGGCGGTTTTCCGAACCATCCCCTCGAAGGTCCTCACTCTGGA
GGAACTGGGCTGTCCGAAATTCTTTCAGGATGTCACCCGCCAGCCGCGCGGGCTGATCGTGGTCACCGGCCCCACCGGTT
CGGGCAAGTCGACCACCCTGGCGGCCATGATCGACTACATCAACTCCAACGACTACTCCCATATCCTGACCATCGAAGAT
CCGATCGAATTCGTGCACCAGAGCAAGAAATGCCTGATCAACCAGCGCGAGGTGCACCGCAACACCCTGGGGTTCAACGA
AGCCCTGCGCTCGGCGCTGCGCGAAGACCCGGACGTGATCCTGGTGGGCGAAATGCGCGATCTGGAAACCATCCGTCTGG
CGCTCACCGCGGCCGAGACCGGACACTTGGTGTTCGGCACCCTGCATACCAGCTCCGCCGCCAAGACCATCGACCGCATC
GTCGACGTATTCCCCGCCGCGGAAAAGGAGATGATCCGCTCCATGCTGTCGGAATCGCTCCAGGCGGTGATCTCGCAGGC
GCTCGTCAAGAAAGCCGGCGGCGGCCGAACCGCGGCTTGGGAGATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GGGAGGCCAAGGTCGCCCAGATGTATTCCGCGATCCAGACCGGCCGCAAGGACGGCATGCAGACCCTGGACCAGCACCTG
CAGGAACTGGTGGAAAAAGGGGTCATCAACCGCCAGGTCGCCCGCGAAATCGCCGTCAACAAGGCCGCATTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A858Q7Q7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

81.657

98.256

0.802
  pilT Pseudomonas stutzeri DSM 10701

79.07

100

0.791
  pilT Acinetobacter nosocomialis M2

78.488

100

0.785
  pilT Acinetobacter baumannii D1279779

78.488

100

0.785
  pilT Acinetobacter baumannii strain A118

78.488

100

0.785
  pilT Acinetobacter baylyi ADP1

77.326

100

0.773
  pilT Legionella pneumophila strain Lp02

74.709

100

0.747
  pilT Legionella pneumophila strain ERS1305867

74.709

100

0.747
  pilT Neisseria meningitidis 8013

68.116

100

0.683
  pilT Neisseria gonorrhoeae MS11

67.536

100

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.957

100

0.672
  pilT Vibrio cholerae strain A1552

66.957

100

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.517
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.384

0.395
  pilU Vibrio cholerae strain A1552

40

97.384

0.39
  pilU Acinetobacter baylyi ADP1

39.755

95.058

0.378

Multiple sequence alignment