Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GNH96_RS07680 Genome accession   NZ_CP046565
Coordinates   1587639..1588769 (-) Length   376 a.a.
NCBI ID   WP_169603140.1    Uniprot ID   A0A858Q7M2
Organism   Methylococcus geothermalis strain IM1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1582639..1593769
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GNH96_RS07650 (GNH96_07620) - 1582647..1582865 (+) 219 WP_407658841.1 glutaredoxin family protein -
  GNH96_RS07655 (GNH96_07625) - 1582885..1584294 (+) 1410 WP_169603135.1 aspartate ammonia-lyase -
  GNH96_RS07660 (GNH96_07630) - 1584327..1585109 (+) 783 WP_169603136.1 succinate dehydrogenase iron-sulfur subunit -
  GNH96_RS07665 (GNH96_07635) - 1585106..1585363 (+) 258 WP_169603137.1 succinate dehydrogenase assembly factor 2 -
  GNH96_RS07670 (GNH96_07640) - 1585357..1585779 (+) 423 WP_169603138.1 hypothetical protein -
  GNH96_RS07675 (GNH96_07645) - 1585790..1587580 (+) 1791 WP_169603139.1 VWA domain-containing protein -
  GNH96_RS07680 (GNH96_07650) pilU 1587639..1588769 (-) 1131 WP_169603140.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GNH96_RS07685 (GNH96_07655) pilT 1588784..1589818 (-) 1035 WP_169603141.1 type IV pilus twitching motility protein PilT Machinery gene
  GNH96_RS07690 (GNH96_07660) - 1589872..1590561 (+) 690 WP_169603142.1 YggS family pyridoxal phosphate-dependent enzyme -
  GNH96_RS07695 (GNH96_07665) proC 1590629..1591456 (+) 828 WP_169603143.1 pyrroline-5-carboxylate reductase -
  GNH96_RS07700 (GNH96_07670) - 1591456..1592034 (+) 579 WP_169603144.1 YggT family protein -
  GNH96_RS07705 (GNH96_07675) - 1592163..1592531 (+) 369 WP_228720067.1 EAL domain-containing protein -
  GNH96_RS07710 (GNH96_07680) trmB 1592516..1593208 (-) 693 WP_169603146.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
  GNH96_RS07715 (GNH96_07685) - 1593296..1593511 (-) 216 WP_169603147.1 DUF2905 domain-containing protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 42242.81 Da        Isoelectric Point: 6.6604

>NTDB_id=405581 GNH96_RS07680 WP_169603140.1 1587639..1588769(-) (pilU) [Methylococcus geothermalis strain IM1]
MEFESLLKLMVLKKASDLFITAAKEPCMKVDGAIVPLSSAKLSAEQARQLVLGIMNQRQRDEFENTNECNFALSVTGLGR
FRVSAFIQRNSPGMVLRRIETEIPTVEQLNLPPILNDLVMTKRGLILFVGATGTGKSTSLAAMIKYRNEHSSGHIITIED
PLEFVHPHAGCIVTQREVGIDTESYEVALKNTLRQAPDVILIGEIRTRETMQQAITFAETGHLCLSTLHANNANQALDRI
LHFFPEDMHPQLFMDLSLNLRAIIAQQLVKRADGKGRYPAVEILINTPLVSDLIRQGEVHKLKDVMKQSREHGMRTFDQA
LFELFKAGKIGYEDALYCADSKNEVRLMIKLSEQGSIDKYAPKDDAIRIVENDEWK

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=405581 GNH96_RS07680 WP_169603140.1 1587639..1588769(-) (pilU) [Methylococcus geothermalis strain IM1]
ATGGAATTCGAATCGCTGCTCAAGCTGATGGTACTGAAGAAAGCGTCGGACTTGTTCATTACGGCGGCCAAGGAGCCCTG
CATGAAGGTCGACGGCGCCATCGTGCCGCTGTCCAGCGCCAAGCTGAGCGCCGAACAGGCGCGCCAACTCGTCCTCGGCA
TCATGAACCAGCGCCAGCGCGACGAGTTCGAGAACACCAACGAATGCAACTTCGCCCTGTCCGTGACCGGGCTTGGGAGG
TTCCGGGTCAGCGCCTTCATCCAGCGCAACAGTCCCGGCATGGTGTTGCGCCGGATCGAAACCGAGATTCCCACCGTGGA
ACAGCTCAACCTGCCGCCCATCCTCAACGATCTGGTCATGACCAAGCGCGGCCTGATCCTGTTCGTGGGCGCGACCGGCA
CCGGCAAGTCCACTTCGCTGGCGGCGATGATCAAGTACCGCAACGAGCACTCCAGCGGCCACATCATCACCATCGAGGAC
CCCCTGGAGTTCGTCCACCCGCACGCAGGCTGCATCGTCACCCAGCGCGAGGTCGGCATCGACACCGAATCCTACGAAGT
CGCGCTGAAGAACACCCTGCGGCAGGCGCCGGACGTGATCCTCATCGGCGAGATCCGCACCCGCGAAACCATGCAGCAGG
CCATCACCTTCGCCGAAACCGGCCACCTCTGCCTGAGCACCCTGCACGCCAACAATGCCAACCAGGCGCTGGACCGCATC
CTCCATTTCTTCCCGGAAGACATGCATCCGCAGCTGTTCATGGACCTGTCGCTGAACCTGCGCGCCATCATCGCCCAGCA
GCTCGTCAAGCGCGCCGACGGCAAGGGACGCTATCCGGCGGTGGAAATCCTCATCAATACCCCTTTGGTCTCGGACCTGA
TCCGCCAGGGCGAGGTCCACAAGCTCAAGGACGTGATGAAACAATCGCGCGAACACGGCATGCGGACCTTCGACCAGGCG
CTGTTCGAACTGTTCAAGGCGGGCAAGATCGGCTACGAGGATGCCCTCTACTGCGCCGATTCCAAGAACGAAGTGCGCCT
GATGATCAAGCTGAGCGAACAGGGCAGTATCGACAAATACGCGCCCAAGGACGACGCCATCCGGATCGTCGAGAACGACG
AGTGGAAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A858Q7M2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.644

94.149

0.646
  pilU Acinetobacter baylyi ADP1

62.634

98.936

0.62
  pilU Vibrio cholerae strain A1552

58.286

93.085

0.543
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.678

92.553

0.404
  pilT Legionella pneumophila strain ERS1305867

40.816

91.223

0.372
  pilT Legionella pneumophila strain Lp02

40.816

91.223

0.372
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.617

88.83

0.37
  pilT Vibrio cholerae strain A1552

41.617

88.83

0.37
  pilT Acinetobacter baylyi ADP1

40.762

90.691

0.37
  pilT Acinetobacter nosocomialis M2

40.413

90.16

0.364
  pilT Acinetobacter baumannii D1279779

40.413

90.16

0.364
  pilT Acinetobacter baumannii strain A118

40.413

90.16

0.364

Multiple sequence alignment