Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CBC4_RS07635 Genome accession   NC_015425
Coordinates   1625366..1626421 (-) Length   351 a.a.
NCBI ID   WP_013725728.1    Uniprot ID   -
Organism   Clostridium botulinum BKT015925     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1620366..1631421
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CBC4_RS07615 (CbC4_1528) sigE 1620962..1621669 (-) 708 WP_029169687.1 RNA polymerase sporulation sigma factor SigE -
  CBC4_RS07620 (CbC4_1529) spoIIGA 1621683..1622474 (-) 792 WP_029169407.1 sigma-E processing peptidase SpoIIGA -
  CBC4_RS07625 (CbC4_1530) ftsZ 1622655..1623842 (-) 1188 WP_013725726.1 cell division protein FtsZ -
  CBC4_RS07630 (CbC4_1531) ftsA 1623858..1625114 (-) 1257 WP_013725727.1 cell division protein FtsA -
  CBC4_RS07635 (CbC4_1532) pilT 1625366..1626421 (-) 1056 WP_013725728.1 type IV pilus twitching motility protein PilT Machinery gene
  CBC4_RS07640 (CbC4_1533) sigK 1626522..1627229 (-) 708 WP_019278307.1 RNA polymerase sporulation sigma factor SigK -
  CBC4_RS07645 (CbC4_1534) - 1627296..1628981 (-) 1686 WP_019278306.1 penicillin-binding transpeptidase domain-containing protein -
  CBC4_RS07650 (CbC4_1535) udk 1629046..1629672 (-) 627 WP_013725731.1 uridine kinase -
  CBC4_RS07655 (CbC4_1536) - 1629676..1630902 (-) 1227 WP_013725732.1 U32 family peptidase -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 38919.03 Da        Isoelectric Point: 8.3383

>NTDB_id=40480 CBC4_RS07635 WP_013725728.1 1625366..1626421(-) (pilT) [Clostridium botulinum BKT015925]
MIPLNELLKKTIDEKASDLHLTVGISPVIRINGELVRVGEQKLTANDTRKYVMEILGDSFEKYDNVGEIDTSISIPSVGR
FRVNIYKQRGSYALAIRAVALKIPSIGQLKLPSIIKELSDKHRGLILVTGPTGSGKSTTLASIINEINNTRSAHIITLED
PIEFLHKHNKSVINQREIGNDTSSYTDALRAALREDPDVILVGEMRDLETMATAITAAETGHLVLSTLHTIGAAKTIDRI
IDVFPPHQQQQIKIQLSTVLQGVISQQLIPKYDGKGQIASFEIMVLNSAIQNLIREGKTHQIQSLIQTGSKYGMKTMDMS
IVELYKSKLISYEDALTYSIDKSMVKRMIDM

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=40480 CBC4_RS07635 WP_013725728.1 1625366..1626421(-) (pilT) [Clostridium botulinum BKT015925]
GTGATACCATTAAATGAGTTATTAAAAAAGACTATAGATGAAAAAGCATCAGATCTACATTTAACTGTTGGAATTTCTCC
GGTAATTAGAATAAATGGTGAACTAGTAAGGGTTGGTGAGCAAAAGCTTACTGCAAATGATACAAGAAAATATGTTATGG
AAATATTGGGGGACTCATTCGAAAAGTATGATAATGTTGGTGAAATAGATACATCTATATCTATTCCTAGTGTTGGAAGA
TTTAGAGTTAATATATATAAACAGAGGGGGAGTTATGCACTTGCTATAAGAGCAGTTGCATTAAAAATACCTTCTATTGG
TCAACTAAAGTTACCCTCTATAATAAAAGAACTTAGTGATAAGCATAGAGGACTTATTCTTGTAACTGGACCAACTGGAA
GTGGTAAGAGTACAACTTTAGCATCTATAATAAACGAAATAAATAATACGAGATCAGCTCATATTATTACATTAGAGGAT
CCTATAGAATTCCTCCATAAGCATAATAAGTCAGTAATTAATCAAAGGGAAATAGGAAATGATACATCATCTTATACTGA
TGCATTAAGAGCAGCACTTAGAGAGGATCCAGATGTAATTCTTGTTGGGGAGATGAGGGATCTTGAAACTATGGCAACGG
CAATTACAGCAGCAGAAACAGGACATTTGGTATTATCTACTCTACATACTATAGGAGCAGCCAAAACTATAGATAGGATA
ATTGATGTTTTCCCACCACATCAACAACAACAAATAAAAATACAACTTTCAACTGTACTTCAAGGTGTTATATCTCAACA
ATTAATACCAAAATATGACGGAAAAGGACAAATAGCCTCATTTGAAATTATGGTATTAAACTCTGCGATACAAAATCTTA
TAAGAGAAGGAAAAACTCATCAAATTCAATCATTAATTCAAACAGGAAGTAAGTATGGTATGAAAACTATGGATATGAGC
ATAGTTGAGCTATATAAAAGTAAATTAATATCATATGAGGATGCTTTAACGTATTCTATTGACAAAAGTATGGTAAAAAG
AATGATAGATATGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(92-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

50.751

94.872

0.481
  pilT Neisseria gonorrhoeae MS11

50.45

94.872

0.479
  pilT Acinetobacter baumannii strain A118

49.254

95.442

0.47
  pilT Acinetobacter nosocomialis M2

49.254

95.442

0.47
  pilT Acinetobacter baumannii D1279779

49.254

95.442

0.47
  pilT Legionella pneumophila strain Lp02

48.529

96.866

0.47
  pilT Legionella pneumophila strain ERS1305867

48.529

96.866

0.47
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.857

99.715

0.467
  pilT Acinetobacter baylyi ADP1

48.649

94.872

0.462
  pilT Pseudomonas aeruginosa PAK

48.649

94.872

0.462
  pilT Vibrio cholerae strain A1552

48.182

94.017

0.453
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.182

94.017

0.453
  pilT Pseudomonas stutzeri DSM 10701

46.847

94.872

0.444
  pilU Acinetobacter baylyi ADP1

41.716

96.296

0.402
  pilU Vibrio cholerae strain A1552

39.65

97.721

0.387
  pilU Pseudomonas stutzeri DSM 10701

38.968

99.43

0.387

Multiple sequence alignment