Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GNX71_RS30435 Genome accession   NZ_CP046508
Coordinates   6530909..6531952 (+) Length   347 a.a.
NCBI ID   WP_013543830.1    Uniprot ID   -
Organism   Variovorax sp. RKNM96     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6525909..6536952
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GNX71_RS30405 (GNX71_30340) - 6526387..6527040 (-) 654 WP_206175864.1 nucleoside/nucleotide kinase family protein -
  GNX71_RS30410 (GNX71_30345) - 6527077..6528348 (-) 1272 WP_176661906.1 Glu/Leu/Phe/Val dehydrogenase -
  GNX71_RS30415 (GNX71_30350) - 6528593..6528928 (+) 336 WP_206175865.1 DUF4148 domain-containing protein -
  GNX71_RS30420 (GNX71_30355) - 6529064..6529537 (+) 474 WP_206175866.1 hypothetical protein -
  GNX71_RS30425 (GNX71_30360) - 6529676..6530143 (+) 468 WP_198788393.1 Lrp/AsnC ligand binding domain-containing protein -
  GNX71_RS30430 (GNX71_30365) - 6530156..6530857 (-) 702 WP_206175867.1 YggS family pyridoxal phosphate-dependent enzyme -
  GNX71_RS30435 (GNX71_30370) pilT 6530909..6531952 (+) 1044 WP_013543830.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GNX71_RS30440 (GNX71_30375) pilU 6531983..6533119 (+) 1137 WP_176661903.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GNX71_RS30445 (GNX71_30380) - 6533132..6534682 (-) 1551 WP_206175868.1 MFS transporter -
  GNX71_RS30450 (GNX71_30385) - 6534679..6535164 (-) 486 WP_206175869.1 MerR family transcriptional regulator -
  GNX71_RS30455 (GNX71_30390) - 6535253..6536161 (+) 909 WP_206175870.1 NAD(P)-dependent oxidoreductase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38194.92 Da        Isoelectric Point: 6.8356

>NTDB_id=404778 GNX71_RS30435 WP_013543830.1 6530909..6531952(+) (pilT) [Variovorax sp. RKNM96]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVNGDVRRINVDALDHKGVHAMVYDIMSDTHRKHYEEFLEVDFSFEIDGLAR
FRVNAFNQARGAAAVFRTIPSKILTLEQLNAPKIFGELALKPRGLVLVTGPTGSGKSTTLAAMVNYLNENEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFSNALRSALREDPDAILVGELRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGEEKEMIRAMLSESLQAVISQTLCKTKDGQSRVAAHEIMLGTPAIRNLIREAKVAQMYSTIQTGQGSGMQTLDQN
LMELVRRNTISAAEARGKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=404778 GNX71_RS30435 WP_013543830.1 6530909..6531952(+) (pilT) [Variovorax sp. RKNM96]
GTGGACATCACCCAACTGCTGGCATTCAGCGTCAAGAACAAAGCCTCCGACCTGCACTTGTCGGCCGGGCTGCCGCCCAT
GATCCGTGTGAACGGCGACGTGCGCCGCATCAATGTCGATGCGCTCGACCACAAGGGCGTGCACGCGATGGTGTACGACA
TCATGAGCGACACGCACCGCAAGCACTACGAAGAGTTCCTGGAGGTCGACTTCTCGTTCGAGATCGACGGCCTCGCGCGC
TTCCGCGTGAACGCCTTCAACCAGGCGCGCGGCGCGGCCGCCGTGTTCCGGACCATTCCCTCGAAGATCCTCACGCTCGA
GCAGCTGAACGCGCCGAAGATTTTCGGCGAACTGGCGCTCAAGCCGCGCGGGCTGGTGCTGGTGACGGGCCCCACGGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGGTGAACTACCTCAACGAAAACGAGTACGGCCACATCCTCACGGTGGAAGAC
CCGATCGAATTCGTGCACGAGTCGAAGAAGTGCCTGATCAACCAGCGCGAAGTGGGCCCGATGACGCTGTCGTTCTCGAA
CGCGCTGCGCTCTGCCCTGCGCGAAGACCCGGACGCCATTCTTGTGGGCGAGCTGCGCGACCTCGAAACCATCCGCCTCG
CGATGACCGCGGCCGAGACGGGCCACCTGGTGTTCGGCACGCTGCACACCTCGTCGGCCGCCAAGACCATCGACCGGATC
ATCGACGTGTTCCCGGGCGAGGAAAAGGAAATGATCCGCGCGATGCTCTCGGAGTCGCTGCAGGCCGTGATTTCGCAGAC
GCTGTGCAAGACCAAGGACGGCCAGAGCCGCGTGGCGGCGCACGAGATCATGCTGGGCACACCCGCCATCCGGAACCTGA
TCCGCGAAGCCAAGGTGGCGCAGATGTACTCCACCATCCAGACCGGCCAGGGCTCCGGCATGCAGACGCTGGACCAGAAC
CTGATGGAGCTGGTTCGCCGCAACACGATTTCCGCTGCCGAAGCCCGCGGCAAGGCAAAGATTCCCGAGAATTTCCCCGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

75.652

99.424

0.752
  pilT Acinetobacter nosocomialis M2

75.362

99.424

0.749
  pilT Acinetobacter baumannii D1279779

75.362

99.424

0.749
  pilT Acinetobacter baumannii strain A118

75.362

99.424

0.749
  pilT Pseudomonas stutzeri DSM 10701

75.072

99.424

0.746
  pilT Acinetobacter baylyi ADP1

73.913

99.424

0.735
  pilT Legionella pneumophila strain Lp02

73.451

97.695

0.718
  pilT Legionella pneumophila strain ERS1305867

73.451

97.695

0.718
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.622

98.271

0.674
  pilT Vibrio cholerae strain A1552

68.622

98.271

0.674
  pilT Neisseria meningitidis 8013

67.246

99.424

0.669
  pilT Neisseria gonorrhoeae MS11

66.957

99.424

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.145

99.424

0.499
  pilU Vibrio cholerae strain A1552

43.881

96.542

0.424
  pilU Acinetobacter baylyi ADP1

41.329

99.712

0.412
  pilU Pseudomonas stutzeri DSM 10701

40.896

96.542

0.395

Multiple sequence alignment