Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GNX71_RS30440 Genome accession   NZ_CP046508
Coordinates   6531983..6533119 (+) Length   378 a.a.
NCBI ID   WP_176661903.1    Uniprot ID   -
Organism   Variovorax sp. RKNM96     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6526983..6538119
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GNX71_RS30410 (GNX71_30345) - 6527077..6528348 (-) 1272 WP_176661906.1 Glu/Leu/Phe/Val dehydrogenase -
  GNX71_RS30415 (GNX71_30350) - 6528593..6528928 (+) 336 WP_206175865.1 DUF4148 domain-containing protein -
  GNX71_RS30420 (GNX71_30355) - 6529064..6529537 (+) 474 WP_206175866.1 hypothetical protein -
  GNX71_RS30425 (GNX71_30360) - 6529676..6530143 (+) 468 WP_198788393.1 Lrp/AsnC ligand binding domain-containing protein -
  GNX71_RS30430 (GNX71_30365) - 6530156..6530857 (-) 702 WP_206175867.1 YggS family pyridoxal phosphate-dependent enzyme -
  GNX71_RS30435 (GNX71_30370) pilT 6530909..6531952 (+) 1044 WP_013543830.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GNX71_RS30440 (GNX71_30375) pilU 6531983..6533119 (+) 1137 WP_176661903.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GNX71_RS30445 (GNX71_30380) - 6533132..6534682 (-) 1551 WP_206175868.1 MFS transporter -
  GNX71_RS30450 (GNX71_30385) - 6534679..6535164 (-) 486 WP_206175869.1 MerR family transcriptional regulator -
  GNX71_RS30455 (GNX71_30390) - 6535253..6536161 (+) 909 WP_206175870.1 NAD(P)-dependent oxidoreductase -
  GNX71_RS30460 (GNX71_30395) - 6536192..6537004 (-) 813 WP_206175871.1 BON domain-containing protein -
  GNX71_RS30465 (GNX71_30400) - 6537001..6537645 (-) 645 WP_198788387.1 SIS domain-containing protein -
  GNX71_RS30470 (GNX71_30405) - 6537732..6538106 (-) 375 WP_206175872.1 YraN family protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41852.90 Da        Isoelectric Point: 6.4186

>NTDB_id=404779 GNX71_RS30440 WP_176661903.1 6531983..6533119(+) (pilU) [Variovorax sp. RKNM96]
MERDQASQFINDLLKLMVSRNGSDLFITADFPPAIKVDGKVTKVSQQALGAQHTLALTRSVMNDRQVADFERTKECNFAI
SPTGIGRFRVNAFVQQGKVGMVLRTIPAKLPTIDGLGMPQILKDVSMTKRGLTILVGATGSGKSTTLAAMIDWRNENSYG
HIVTVEDPVEFVHPHKNCVVTQREVGIDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRSLVSQRLVPTEDGQGRVAAVEVLLNTPLISDLIFKGEVGEIKEIMRKSRNLG
MQTFDQALFDLFESHSITFEDAIRNADSANDLRLQIKLNSQRARSTDLAAGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=404779 GNX71_RS30440 WP_176661903.1 6531983..6533119(+) (pilU) [Variovorax sp. RKNM96]
ATGGAACGCGATCAAGCCAGCCAGTTCATCAACGACCTGCTCAAGCTCATGGTGAGCCGCAACGGCAGCGACCTGTTCAT
CACCGCCGACTTTCCGCCCGCGATCAAGGTCGATGGCAAGGTCACCAAGGTGTCGCAGCAGGCGCTGGGTGCGCAGCACA
CGCTGGCGCTCACGCGCTCGGTCATGAACGACCGACAGGTGGCCGATTTCGAGCGCACCAAGGAGTGCAACTTCGCGATC
TCGCCGACCGGCATCGGGCGCTTTCGCGTCAACGCCTTCGTGCAGCAGGGCAAGGTCGGCATGGTGTTGCGGACCATTCC
CGCCAAGCTGCCGACCATCGACGGCCTGGGCATGCCGCAGATCCTGAAGGACGTGTCGATGACCAAGCGCGGCCTCACCA
TCCTGGTGGGCGCCACCGGTTCGGGCAAGTCAACCACGCTGGCCGCGATGATCGACTGGCGCAACGAGAACTCCTACGGC
CACATCGTCACGGTGGAAGACCCGGTCGAGTTCGTGCACCCGCACAAGAACTGCGTGGTGACGCAGCGCGAAGTGGGCAT
CGACACCGACAGCTGGGAAGCGGCGCTCAAGAACACGCTGCGCCAGGCACCCGACGTGATCCTGATGGGCGAAATCCGCG
ACCGCGAAACCATGGAACACGCGGTGGCCTTCGCCGAAACCGGGCATCTGTGCATGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGGATCATCAACTTCTTCCCCGAAGAGCGCCGCGCGCAGCTCCTGATGGACCTGTCGCTGAACCT
GCGCTCGCTGGTCTCGCAGCGCCTGGTGCCCACCGAAGACGGCCAGGGCCGCGTGGCCGCCGTCGAGGTGCTGCTGAACA
CGCCGCTGATCTCCGACCTGATCTTCAAGGGCGAGGTGGGCGAGATCAAGGAGATCATGCGCAAGAGCCGCAACCTGGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGACCTGTTCGAGAGCCACTCGATCACCTTCGAGGACGCCATCCGCAACGCCGA
CTCGGCCAACGATCTGCGGCTGCAGATCAAGCTCAACAGCCAGCGTGCGCGCAGCACGGACCTGGCAGCCGGCACGGAGC
ACTTCGCGATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(50-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.61

92.328

0.587
  pilU Acinetobacter baylyi ADP1

57.542

94.709

0.545
  pilU Vibrio cholerae strain A1552

52.342

96.032

0.503
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.994

89.153

0.41
  pilT Pseudomonas aeruginosa PAK

44.807

89.153

0.399
  pilT Acinetobacter nosocomialis M2

44.144

88.095

0.389
  pilT Pseudomonas stutzeri DSM 10701

42.73

89.153

0.381
  pilT Acinetobacter baumannii D1279779

45.141

84.392

0.381
  pilT Acinetobacter baumannii strain A118

45.141

84.392

0.381
  pilT Acinetobacter baylyi ADP1

42.943

88.095

0.378
  pilT Legionella pneumophila strain Lp02

43.465

87.037

0.378
  pilT Legionella pneumophila strain ERS1305867

43.465

87.037

0.378

Multiple sequence alignment