Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ALIDE2_RS02250 Genome accession   NC_015422
Coordinates   464867..465910 (-) Length   347 a.a.
NCBI ID   WP_013517392.1    Uniprot ID   A0A858ZQD7
Organism   Alicycliphilus denitrificans K601     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 459867..470910
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ALIDE2_RS02220 (Alide2_0444) - 460246..460644 (+) 399 WP_013721280.1 YraN family protein -
  ALIDE2_RS02225 (Alide2_0445) - 460705..461307 (+) 603 WP_013517387.1 SIS domain-containing protein -
  ALIDE2_RS02230 (Alide2_0446) - 461304..461969 (+) 666 WP_013517388.1 BON domain-containing protein -
  ALIDE2_RS02235 (Alide2_0447) - 461998..462930 (-) 933 WP_013721281.1 NAD(P)-dependent oxidoreductase -
  ALIDE2_RS02240 (Alide2_0448) pilU 463011..464147 (-) 1137 WP_013517390.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ALIDE2_RS02245 (Alide2_0449) - 464173..464802 (-) 630 WP_013517391.1 cyclic nucleotide-binding domain-containing protein -
  ALIDE2_RS02250 (Alide2_0450) pilT 464867..465910 (-) 1044 WP_013517392.1 type IV pilus twitching motility protein PilT Machinery gene
  ALIDE2_RS02255 (Alide2_0451) - 465950..466672 (+) 723 WP_013721282.1 YggS family pyridoxal phosphate-dependent enzyme -
  ALIDE2_RS02260 (Alide2_0452) - 466713..467843 (+) 1131 WP_013517394.1 aminotransferase class V-fold PLP-dependent enzyme -
  ALIDE2_RS02265 (Alide2_0453) - 467904..468326 (-) 423 WP_013517395.1 hypothetical protein -
  ALIDE2_RS02270 (Alide2_0454) - 468511..469812 (+) 1302 WP_013721283.1 DUF445 domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38178.96 Da        Isoelectric Point: 6.8355

>NTDB_id=40425 ALIDE2_RS02250 WP_013517392.1 464867..465910(-) (pilT) [Alicycliphilus denitrificans K601]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDALDHKTVHAMVYDIMSDAQRKVYEEFLEVDFSFEIEGLAR
FRVNAFNQNRGAAAVFRTIPSKILTLEQLNAPKIFADLALKPRGLVLVTGPTGSGKSTTLAGMINHLNETEYGHILTIED
PIEFVHESKKCLVNQREVGPMTLSFSAALRSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKLKDGSGRVAAHEIMTGTSAIRNLIREAKVAQMYSTIQTSNSVGMQTLDQN
LTDLVRRNIISPAEARSKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=40425 ALIDE2_RS02250 WP_013517392.1 464867..465910(-) (pilT) [Alicycliphilus denitrificans K601]
GTGGACATCACCCAGTTGCTCGCCTTCAGCGTGAAGAACAAGGCGTCCGACCTGCATCTGTCGGCCGGCCTTCCTCCGAT
GATCCGGGTGCATGGTGATGTGCGGCGCATCAACGTCGATGCGCTGGACCACAAGACCGTCCATGCCATGGTGTACGACA
TCATGAGCGATGCCCAGCGCAAGGTCTACGAAGAGTTCCTGGAGGTGGACTTCTCCTTCGAGATCGAGGGCCTGGCGCGC
TTCCGGGTCAACGCCTTCAACCAGAACCGCGGCGCGGCGGCGGTCTTCCGCACCATCCCGAGCAAGATCCTGACGCTGGA
GCAGCTCAACGCCCCCAAGATCTTCGCGGACCTGGCGCTCAAGCCCCGCGGCCTGGTGCTGGTGACCGGCCCCACGGGCT
CGGGCAAGTCCACCACGCTGGCCGGCATGATCAACCACCTCAACGAAACCGAGTACGGCCACATCCTCACCATCGAGGAC
CCGATCGAGTTCGTGCACGAGTCCAAGAAATGCCTGGTCAACCAGCGCGAGGTGGGGCCGATGACGCTGTCGTTCTCCGC
TGCGCTGCGCTCGGCGCTGCGCGAGGACCCGGACGCCATCCTCGTGGGCGAAATGCGCGACCTGGAGACCATCCGCCTGG
CCATGACGGCGGCCGAAACGGGCCACCTGGTGTTCGGCACGCTGCACACCTCCAGCGCCGCCAAGACGATCGACCGCATC
ATCGACGTGTTCCCGGCCGAGGAGAAGGAGATGGTGCGCGCCATGCTGTCGGAGTCGCTGCAGGCCGTGATCTCGCAGAC
CCTGTGCAAGCTCAAGGACGGTTCGGGCCGCGTGGCGGCGCACGAGATCATGACGGGCACCAGCGCCATCCGCAACCTGA
TCCGCGAAGCCAAGGTGGCGCAGATGTACTCCACCATCCAGACCAGCAACAGCGTCGGCATGCAGACGCTCGACCAGAAT
CTCACCGACCTGGTGCGCCGCAACATCATCAGTCCCGCCGAGGCGCGCAGCAAGGCCAAGATCCCGGAAAATTTCCCCGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A858ZQD7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.391

99.424

0.769
  pilT Pseudomonas stutzeri DSM 10701

76.812

99.424

0.764
  pilT Acinetobacter nosocomialis M2

75.652

99.424

0.752
  pilT Acinetobacter baumannii D1279779

75.652

99.424

0.752
  pilT Acinetobacter baumannii strain A118

75.652

99.424

0.752
  pilT Acinetobacter baylyi ADP1

74.493

99.424

0.741
  pilT Legionella pneumophila strain Lp02

73.746

97.695

0.72
  pilT Legionella pneumophila strain ERS1305867

73.746

97.695

0.72
  pilT Neisseria meningitidis 8013

67.536

99.424

0.671
  pilT Neisseria gonorrhoeae MS11

67.246

99.424

0.669
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.862

98.271

0.657
  pilT Vibrio cholerae strain A1552

66.862

98.271

0.657
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

99.424

0.504
  pilU Vibrio cholerae strain A1552

43.284

96.542

0.418
  pilU Acinetobacter baylyi ADP1

41.329

99.712

0.412
  pilU Pseudomonas stutzeri DSM 10701

41.493

96.542

0.401

Multiple sequence alignment