Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ALIDE2_RS02240 Genome accession   NC_015422
Coordinates   463011..464147 (-) Length   378 a.a.
NCBI ID   WP_013517390.1    Uniprot ID   A0A858ZPA8
Organism   Alicycliphilus denitrificans K601     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 458011..469147
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ALIDE2_RS02210 (Alide2_0442) - 458409..459332 (+) 924 WP_013721278.1 lysylphosphatidylglycerol synthase transmembrane domain-containing protein -
  ALIDE2_RS02215 (Alide2_0443) rsmI 459329..460246 (-) 918 WP_041700574.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  ALIDE2_RS02220 (Alide2_0444) - 460246..460644 (+) 399 WP_013721280.1 YraN family protein -
  ALIDE2_RS02225 (Alide2_0445) - 460705..461307 (+) 603 WP_013517387.1 SIS domain-containing protein -
  ALIDE2_RS02230 (Alide2_0446) - 461304..461969 (+) 666 WP_013517388.1 BON domain-containing protein -
  ALIDE2_RS02235 (Alide2_0447) - 461998..462930 (-) 933 WP_013721281.1 NAD(P)-dependent oxidoreductase -
  ALIDE2_RS02240 (Alide2_0448) pilU 463011..464147 (-) 1137 WP_013517390.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ALIDE2_RS02245 (Alide2_0449) - 464173..464802 (-) 630 WP_013517391.1 cyclic nucleotide-binding domain-containing protein -
  ALIDE2_RS02250 (Alide2_0450) pilT 464867..465910 (-) 1044 WP_013517392.1 type IV pilus twitching motility protein PilT Machinery gene
  ALIDE2_RS02255 (Alide2_0451) - 465950..466672 (+) 723 WP_013721282.1 YggS family pyridoxal phosphate-dependent enzyme -
  ALIDE2_RS02260 (Alide2_0452) - 466713..467843 (+) 1131 WP_013517394.1 aminotransferase class V-fold PLP-dependent enzyme -
  ALIDE2_RS02265 (Alide2_0453) - 467904..468326 (-) 423 WP_013517395.1 hypothetical protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41618.06 Da        Isoelectric Point: 6.9078

>NTDB_id=40424 ALIDE2_RS02240 WP_013517390.1 463011..464147(-) (pilU) [Alicycliphilus denitrificans K601]
MERDQASKFINDLLKLMVSRGGSDLFITADFPPAIKVDGKVTKVSPQPLTPVHTLTLARSIMSDKQVADFERTKECNFAI
APAGIGRFRANAFIQQGRVGMVLRTIPLTLPTIDGLGVPQVLKEIAMTKRGLCILVGATGSGKSTTLAAMVDWRNENSFG
HIVTVEDPIEFVHPHKNCVVTQREVGLDTDSWEAALKNSLRQAPDVILMGEIRDRETMEHAVAFAETGHLCLATLHANSA
NQALDRIVNFFPEERRAQLLMDLSLNLKAMVSQRLVPKQDGKGRAAAVEVMLNTPLIADLIFKGEVAEIKEIMKKSRNLG
MQTFDQSLFDLFEANVIGYEDALRNADSINDLRLQIKLSSQRAKTTDLASGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=40424 ALIDE2_RS02240 WP_013517390.1 463011..464147(-) (pilU) [Alicycliphilus denitrificans K601]
ATGGAACGCGACCAGGCCAGCAAATTCATCAACGACCTGCTCAAACTGATGGTCAGCCGGGGCGGCAGCGACCTGTTCAT
CACGGCCGACTTTCCGCCGGCCATCAAGGTGGACGGCAAGGTCACCAAGGTCTCGCCCCAGCCGCTCACGCCCGTGCACA
CGCTGACGCTGGCGCGCTCCATCATGAGCGACAAGCAGGTGGCCGACTTCGAGCGCACCAAGGAGTGCAACTTCGCCATC
GCCCCCGCGGGCATCGGCCGCTTTCGCGCCAACGCCTTCATCCAGCAGGGCCGCGTGGGCATGGTGCTGCGCACCATCCC
GCTGACGCTGCCCACCATCGACGGCCTGGGCGTGCCGCAGGTGCTCAAGGAAATCGCCATGACCAAACGCGGCCTGTGCA
TCCTGGTGGGCGCAACGGGCTCGGGCAAGTCCACCACGCTGGCCGCCATGGTGGACTGGCGCAACGAGAACTCCTTCGGC
CACATCGTCACCGTCGAGGATCCGATCGAATTCGTGCACCCGCACAAGAACTGCGTGGTCACGCAGCGCGAGGTGGGCCT
GGACACCGACAGCTGGGAGGCGGCACTCAAGAACTCGCTGCGCCAGGCGCCCGACGTGATCCTGATGGGCGAGATCCGCG
ACCGCGAGACCATGGAGCACGCCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGCATCGTCAACTTCTTCCCCGAGGAGCGGCGCGCCCAGCTGCTCATGGACCTGTCGCTGAACCT
CAAGGCCATGGTCTCGCAGCGCCTGGTTCCCAAGCAGGACGGCAAGGGCCGCGCAGCGGCCGTCGAGGTCATGCTCAACA
CCCCGCTGATCGCGGACCTGATCTTCAAGGGCGAAGTCGCCGAAATCAAGGAGATCATGAAGAAGAGCCGCAACCTGGGC
ATGCAGACCTTCGACCAGTCGCTGTTCGACCTGTTCGAGGCCAACGTCATCGGCTACGAGGATGCCTTGCGCAACGCCGA
TTCGATCAACGATCTGCGCCTGCAGATCAAGCTCAGCAGCCAGCGCGCCAAGACCACCGACCTGGCCTCGGGCACCGAGC
ATTTCGCCATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(121-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A858ZPA8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.183

92.328

0.593
  pilU Acinetobacter baylyi ADP1

57.263

94.709

0.542
  pilU Vibrio cholerae strain A1552

52.893

96.032

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.697

89.153

0.407
  pilT Pseudomonas aeruginosa PAK

44.807

89.153

0.399
  pilT Acinetobacter nosocomialis M2

44.745

88.095

0.394
  pilT Acinetobacter baumannii D1279779

44.745

88.095

0.394
  pilT Acinetobacter baumannii strain A118

44.745

88.095

0.394
  pilT Legionella pneumophila strain ERS1305867

44.144

88.095

0.389
  pilT Legionella pneumophila strain Lp02

44.144

88.095

0.389
  pilT Pseudomonas stutzeri DSM 10701

43.62

89.153

0.389
  pilT Acinetobacter baylyi ADP1

43.293

86.772

0.376
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.867

87.831

0.368
  pilT Vibrio cholerae strain A1552

41.867

87.831

0.368

Multiple sequence alignment