Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MARKY_RS10625 Genome accession   NC_015387
Coordinates   2126894..2128009 (-) Length   371 a.a.
NCBI ID   WP_013704881.1    Uniprot ID   F2NPR0
Organism   Marinithermus hydrothermalis DSM 14884     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2121894..2133009
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MARKY_RS10610 (Marky_2109) - 2122566..2123668 (-) 1103 Protein_2083 transposase -
  MARKY_RS10615 (Marky_2110) - 2123969..2125624 (+) 1656 WP_013704879.1 30S ribosomal protein S1 -
  MARKY_RS10620 (Marky_2111) - 2125680..2126882 (-) 1203 WP_013704880.1 adenylosuccinate synthase -
  MARKY_RS10625 (Marky_2112) pilT 2126894..2128009 (-) 1116 WP_013704881.1 type IV pilus twitching motility protein PilT Machinery gene
  MARKY_RS10630 (Marky_2113) pilF 2128023..2130677 (-) 2655 WP_013704882.1 type II/IV secretion system protein Machinery gene
  MARKY_RS10635 (Marky_2114) - 2130781..2131266 (-) 486 WP_013704883.1 YqeG family HAD IIIA-type phosphatase -
  MARKY_RS10640 (Marky_2115) pgeF 2131284..2132015 (-) 732 WP_013704884.1 peptidoglycan editing factor PgeF -
  MARKY_RS10645 (Marky_2116) - 2132090..2132494 (+) 405 WP_013704885.1 DUF4395 domain-containing protein -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 41257.46 Da        Isoelectric Point: 6.8442

>NTDB_id=40328 MARKY_RS10625 WP_013704881.1 2126894..2128009(-) (pilT) [Marinithermus hydrothermalis DSM 14884]
MEKTPDIVKLLELTVQRGASDLVLTVGVPPMLKVDGEFHPTEFEPLTPQDTRRLMYAMMDEKQQRQFEENKELDFSFSLP
GKGWFRVNVFFQRGSVGGVLRAIPAVIKGFDELGLPKNIAEIALSPRGLVLVTGPTGSGKSTTLAAMVDYINENRRVHIV
TIEDPIEFFHKHKMAIVNQREVGTDTHDFSRALRSVLRQAPDVILVGEMRDYETIRAAITAAETGHLVMGTLHTNSAPET
IDRIVDVFPEEQQEQVRVQLANNLVAVLTQQLLPKAHGDGRVLAYELMIATPAVRALIREGKTHQLPSVIQTGGQYGMIT
MDAHLADLYRRKLITYEVGLARAVDPKEFSRLAGMQEPSPSSARRRPGFSG

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=40328 MARKY_RS10625 WP_013704881.1 2126894..2128009(-) (pilT) [Marinithermus hydrothermalis DSM 14884]
ATGGAAAAAACGCCGGACATCGTGAAACTGCTGGAGCTTACGGTTCAACGGGGCGCTTCGGACCTGGTCTTGACGGTAGG
AGTGCCGCCCATGCTCAAGGTGGACGGGGAGTTTCACCCCACGGAGTTCGAGCCCTTGACCCCGCAGGACACGCGGCGGT
TGATGTACGCCATGATGGACGAGAAGCAGCAGCGCCAGTTCGAGGAGAACAAGGAGCTGGACTTCTCGTTCAGCCTGCCG
GGCAAGGGCTGGTTCCGGGTGAACGTCTTCTTTCAGCGGGGCTCGGTCGGCGGGGTGCTCCGCGCCATCCCCGCGGTGAT
CAAGGGGTTCGACGAGCTGGGCCTGCCCAAGAACATCGCGGAGATCGCCTTGAGCCCGCGCGGCCTGGTCCTGGTCACGG
GCCCCACCGGCTCGGGTAAGTCCACCACCCTCGCCGCGATGGTGGACTACATCAACGAGAACCGCCGGGTGCACATCGTG
ACGATCGAGGACCCCATCGAGTTCTTCCACAAGCACAAGATGGCCATCGTCAACCAGCGCGAGGTGGGGACCGACACCCA
CGACTTCTCCCGGGCGCTGCGCAGCGTGCTGCGCCAAGCCCCGGACGTGATCCTCGTGGGGGAGATGCGCGACTACGAAA
CGATCCGCGCGGCGATCACCGCGGCGGAGACCGGGCATTTGGTGATGGGCACGCTGCACACCAACTCCGCGCCGGAAACC
ATCGACCGCATCGTGGACGTCTTCCCCGAGGAGCAACAGGAGCAGGTGCGCGTCCAGCTCGCGAACAACCTGGTCGCGGT
CCTGACGCAACAACTCCTGCCCAAAGCGCACGGCGACGGCCGCGTCCTCGCCTACGAGCTCATGATCGCCACGCCCGCGG
TGCGGGCCCTGATCCGCGAGGGCAAGACCCACCAGCTGCCCTCCGTCATCCAGACCGGCGGCCAGTACGGCATGATCACC
ATGGACGCGCACCTCGCGGACCTGTACCGCCGCAAGCTCATCACCTACGAGGTCGGCCTCGCCCGCGCAGTGGATCCCAA
GGAGTTCAGCCGCCTCGCGGGGATGCAAGAACCCAGCCCAAGCTCCGCGCGGCGGCGCCCCGGCTTTTCGGGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F2NPR0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

68.678

93.801

0.644
  pilT Acinetobacter nosocomialis M2

54.651

92.722

0.507
  pilT Acinetobacter baumannii D1279779

54.651

92.722

0.507
  pilT Acinetobacter baumannii strain A118

54.651

92.722

0.507
  pilT Acinetobacter baylyi ADP1

54.651

92.722

0.507
  pilT Pseudomonas aeruginosa PAK

54.07

92.722

0.501
  pilT Pseudomonas stutzeri DSM 10701

53.779

92.722

0.499
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.464

92.992

0.488
  pilT Vibrio cholerae strain A1552

52.464

92.992

0.488
  pilT Legionella pneumophila strain Lp02

51.453

92.722

0.477
  pilT Legionella pneumophila strain ERS1305867

51.453

92.722

0.477
  pilT Neisseria meningitidis 8013

50.291

92.722

0.466
  pilT Neisseria gonorrhoeae MS11

49.709

92.722

0.461
  pilU Pseudomonas stutzeri DSM 10701

48.06

90.296

0.434
  pilU Vibrio cholerae strain A1552

47.097

83.558

0.394
  pilU Acinetobacter baylyi ADP1

41.739

92.992

0.388

Multiple sequence alignment