Detailed information NTG0040329

Bioinformatically predicted

Overview

• Name: pilF
• Type: Machinery gene
• Locus tag: MARKY_RS10630
• Genome accession: NC_015387
• Coordinates: 2128023..2130677 (-)
• Length: 884 a.a.
• NCBI ID: WP_013704882.1
• Uniprot ID: F2NPR1
• Organism: Marinithermus hydrothermalis DSM 14884
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2123023..2135677

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MARKY_RS10615 (Marky_2110)	-	2123969..2125624 (+)	1656	WP_013704879.1	30S ribosomal protein S1	-
MARKY_RS10620 (Marky_2111)	-	2125680..2126882 (-)	1203	WP_013704880.1	adenylosuccinate synthase	-
MARKY_RS10625 (Marky_2112)	pilT	2126894..2128009 (-)	1116	WP_013704881.1	type IV pilus twitching motility protein PilT	Machinery gene
MARKY_RS10630 (Marky_2113)	pilF	2128023..2130677 (-)	2655	WP_013704882.1	type II/IV secretion system protein	Machinery gene
MARKY_RS10635 (Marky_2114)	-	2130781..2131266 (-)	486	WP_013704883.1	YqeG family HAD IIIA-type phosphatase	-
MARKY_RS10640 (Marky_2115)	pgeF	2131284..2132015 (-)	732	WP_013704884.1	peptidoglycan editing factor PgeF	-
MARKY_RS10645 (Marky_2116)	-	2132090..2132494 (+)	405	WP_013704885.1	DUF4395 domain-containing protein	-
MARKY_RS10650 (Marky_2117)	-	2132486..2133061 (-)	576	WP_013704886.1	peroxidase-related enzyme	-
MARKY_RS10655 (Marky_2118)	-	2133072..2133668 (-)	597	WP_342626873.1	CoA pyrophosphatase	-
MARKY_RS10660 (Marky_2119)	-	2133593..2134189 (-)	597	WP_013704888.1	MazG family protein	-
MARKY_RS10665 (Marky_2120)	-	2134194..2135282 (-)	1089	WP_013704889.1	A24 family peptidase	-

Sequence

Protein

• Length: 884 a.a.
• Molecular weight: 98809.22 Da
• Isoelectric Point: 4.8977

>NTDB_id=40329 MARKY_RS10630 WP_013704882.1 2128023..2130677(-) (pilF) [Marinithermus hydrothermalis DSM 14884]
MSVLTIGDKRLGAALIELGYLTDEDLQRALEQHREIGGRLADVIVDLGLLSERRIAQAIEAAFGIPLVDLHTLEIPPEVR
ALLPAEKAQELEAIPFALEDKTLRVAFTDPLDNLKIDEVEEITEHIVEPYQTLRASFQYALAKNYPELGLPLPPPPKHTN
PSELKLGELLVHKGLITRETLEQALVQQEKTGELLGKILIQAGKITEADLYRALAEQAGIAFLEDTAGIEADPEIASLFL
RTDALRYRAIPVAEEGGEVTVILGETRHQEEVRRILNRPVRFFLTTPTAWEQLFKRTYPEHARLGEILVQEGKLEREDLQ
QALKVQQRLGRSRSIGEVLVELGYVTPEDIEEALQKQRAGGGRLEDTLVQSGKINPEMLAKSIAIQQGYPYIDPQETPPD
PGVVMLVPEATARRYTVFPHHLEGDALVVLMKDPRNIFAIDDLRMFTKREIKPAVATEDAILKLIERFYGHTGELDELAK
EFARERPEEEQLEASNLDDNAIVRLVNTIIREAYLQEASDIHIEPRQDHVLVRIRVDGALREYTRLPKGTGPAIASRVKI
MGHLDIAERRLPQDGRVRYRDHSIDLDLRLSTLPTVYGEKIVMRLLRRAADIPEIEQLGFAPSVFERFQEVIQKPYGIFL
ITGPTGSGKSFTTFSILKRIATPDKNTTTVEDPVEYEIPGINQTQVNPQAGLTFARALRAFLRQDPDIIMVGEIRDQETA
QIATEAALTGHLVIATLHTNDAAGAITRLEEMGIELFNISASLIGVLAQRLVRRVCDHCRVEVDPDPAVLKSLELSERDL
EGKTLYRGVGCERCGGTGYRGRYAIHELLVVDDAIRQAIVAGKSATEIKALARERGMRTLREDGIYKALEGITTLEEVMA
RTLE

Nucleotide

• Length: 2655 bp

>NTDB_id=40329 MARKY_RS10630 WP_013704882.1 2128023..2130677(-) (pilF) [Marinithermus hydrothermalis DSM 14884]
ATGAGCGTCTTAACGATCGGCGACAAGCGGTTGGGTGCCGCCCTTATCGAGCTCGGGTACCTCACGGATGAGGACCTCCA
GCGCGCTTTGGAGCAGCACCGAGAGATCGGAGGGCGGCTGGCCGACGTCATCGTGGACCTGGGCCTCCTCTCAGAGCGCC
GCATCGCCCAGGCCATCGAGGCCGCGTTCGGCATCCCCCTCGTGGACCTGCACACCCTCGAGATCCCCCCGGAGGTCCGG
GCGCTCCTGCCGGCGGAGAAGGCCCAGGAGCTCGAGGCGATCCCCTTCGCCCTCGAGGACAAGACCCTGCGCGTGGCCTT
CACCGACCCGCTCGACAACCTCAAGATCGACGAGGTCGAGGAGATCACCGAGCACATCGTGGAGCCGTACCAGACGCTAA
GGGCCTCGTTCCAGTACGCCCTCGCTAAGAACTACCCTGAGCTGGGCCTGCCCCTTCCGCCGCCCCCCAAGCACACGAAC
CCCAGCGAGCTGAAGCTCGGTGAGCTGCTGGTGCATAAGGGCCTCATCACCCGCGAGACGTTAGAGCAAGCCTTGGTGCA
GCAGGAAAAAACCGGGGAGCTGCTCGGCAAGATCCTGATCCAGGCCGGGAAGATCACGGAGGCGGACCTGTACCGCGCGC
TCGCGGAACAAGCCGGGATCGCCTTCCTCGAGGACACCGCGGGGATCGAGGCCGACCCCGAGATCGCGAGCTTATTCCTA
CGCACGGACGCCCTGCGCTACCGCGCGATTCCCGTGGCGGAGGAAGGGGGTGAGGTCACGGTCATCCTCGGGGAGACGCG
GCACCAGGAGGAGGTGCGCCGCATCCTGAACCGTCCCGTCCGCTTCTTCCTCACGACCCCTACGGCGTGGGAGCAGCTCT
TCAAGCGCACCTACCCCGAGCACGCCCGGCTGGGTGAGATCCTGGTGCAGGAGGGGAAGCTGGAGCGCGAGGACCTCCAG
CAGGCCCTTAAGGTGCAGCAGCGCCTGGGCCGCAGCCGGTCCATCGGCGAGGTGCTGGTCGAGCTGGGGTACGTCACCCC
CGAGGACATCGAGGAGGCGCTGCAAAAACAACGCGCGGGCGGCGGGCGGCTGGAGGACACCCTGGTCCAGTCGGGCAAGA
TCAACCCCGAGATGCTCGCGAAAAGCATCGCGATCCAGCAAGGCTACCCGTACATCGATCCGCAGGAAACCCCGCCGGAC
CCTGGGGTCGTGATGCTGGTGCCCGAGGCCACGGCGCGCCGGTACACGGTCTTCCCCCACCACCTCGAGGGCGACGCCTT
GGTGGTGTTGATGAAGGACCCGCGGAACATCTTCGCCATCGACGACCTGAGGATGTTCACCAAGCGGGAGATCAAGCCCG
CCGTCGCCACCGAGGACGCGATCCTCAAGCTCATCGAGCGCTTCTACGGTCACACCGGGGAGCTGGACGAGCTCGCGAAG
GAGTTCGCGCGGGAACGTCCCGAGGAGGAGCAGCTCGAGGCCTCGAACCTGGACGACAACGCGATCGTCCGGCTGGTGAA
CACCATCATCCGCGAGGCGTACCTGCAGGAAGCCTCGGACATCCACATCGAGCCGCGCCAGGACCACGTCCTGGTGCGCA
TCCGCGTGGACGGCGCGCTCCGGGAGTACACCCGCCTGCCCAAAGGCACGGGCCCAGCCATCGCCAGCCGCGTCAAGATC
ATGGGGCACCTGGACATCGCCGAGCGGCGCCTGCCCCAGGACGGGCGCGTGCGGTACCGGGACCACTCGATCGACCTGGA
CCTGCGCCTCTCCACGCTGCCCACCGTGTACGGCGAGAAGATCGTGATGCGCCTCTTGCGCCGCGCAGCGGACATCCCCG
AGATCGAGCAGCTCGGCTTCGCCCCCAGCGTCTTCGAACGCTTCCAGGAGGTCATCCAAAAACCCTACGGGATCTTCCTA
ATCACCGGCCCGACCGGTTCGGGCAAGTCGTTCACCACCTTCTCCATCCTCAAGCGCATCGCCACCCCCGACAAGAACAC
CACCACGGTCGAGGACCCGGTCGAGTACGAGATTCCCGGGATCAACCAGACCCAGGTCAACCCCCAGGCGGGCCTCACCT
TCGCCCGGGCGTTGCGCGCCTTTCTGCGCCAGGACCCGGACATCATCATGGTGGGCGAGATCCGCGACCAAGAAACCGCG
CAGATCGCTACCGAGGCCGCACTCACCGGCCACCTGGTCATCGCCACGCTGCACACCAACGACGCGGCCGGCGCGATCAC
CCGGCTGGAGGAGATGGGGATCGAGCTCTTCAACATCTCCGCCTCCCTCATCGGGGTGCTCGCCCAGCGCCTCGTGCGCC
GGGTCTGCGACCACTGCCGGGTCGAGGTAGACCCCGACCCCGCGGTCCTCAAGAGCCTCGAGCTCAGCGAGCGGGACCTC
GAGGGCAAAACCCTCTACCGCGGCGTGGGGTGCGAGCGCTGCGGCGGCACCGGGTACCGGGGCCGGTACGCGATCCACGA
GCTTTTGGTGGTGGACGACGCGATCCGCCAGGCGATCGTCGCGGGCAAATCCGCCACCGAGATCAAGGCCCTCGCGCGCG
AGCGCGGCATGCGAACGCTGCGGGAGGACGGGATCTACAAGGCCCTCGAGGGCATCACCACCCTGGAAGAGGTCATGGCA
CGGACCCTCGAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (508-774)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (45-146)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (368-474)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (199-302)

3D structure

• AlphaFold DB: F2NPR1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilF	Thermus thermophilus HB27	70.819	100	0.714
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	57.675	100	0.578