Detailed information NTG0403153

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: HUW39_RS04560
• Genome accession: NZ_CP056036
• Coordinates: 950634..951614 (+)
• Length: 326 a.a.
• NCBI ID: WP_001542340.1
• Uniprot ID: -
• Organism: Escherichia coli strain EC31
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 945634..956614

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HUW39_RS04530 (HUW39_04535)	-	946012..946998 (-)	987	WP_000784004.1	TRAP transporter substrate-binding protein	-
HUW39_RS04535 (HUW39_04540)	hemW	947311..948447 (-)	1137	WP_001546994.1	radical SAM family heme chaperone HemW	-
HUW39_RS04540 (HUW39_04545)	rdgB	948440..949033 (-)	594	WP_001174747.1	XTP/dITP diphosphatase	-
HUW39_RS04545 (HUW39_04550)	yggU	949041..949331 (-)	291	WP_001277229.1	DUF167 family protein YggU	-
HUW39_RS04550 (HUW39_04555)	yggT	949328..949894 (-)	567	WP_001094831.1	osmotic shock tolerance protein YggT	-
HUW39_RS04555 (HUW39_04560)	yggS	949912..950616 (-)	705	WP_000997795.1	pyridoxal phosphate homeostasis protein	-
HUW39_RS04560 (HUW39_04565)	pilT	950634..951614 (+)	981	WP_001542340.1	type IV pilus twitching motility protein PilT	Machinery gene
HUW39_RS04565 (HUW39_04570)	ruvX	951729..952145 (-)	417	WP_001542339.1	Holliday junction resolvase RuvX	-
HUW39_RS04570 (HUW39_04575)	yqgE	952145..952708 (-)	564	WP_001053178.1	YqgE/AlgH family protein	-
HUW39_RS04575 (HUW39_04580)	gshB	952817..953767 (-)	951	WP_000593273.1	glutathione synthase	-
HUW39_RS04580 (HUW39_04585)	rsmE	953780..954511 (-)	732	WP_001222509.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
HUW39_RS04585 (HUW39_04590)	endA	954591..955298 (-)	708	WP_000286497.1	deoxyribonuclease I	-
HUW39_RS04590 (HUW39_04595)	yggI	955393..955890 (-)	498	WP_001300769.1	SprT family zinc-dependent metalloprotease	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35958.12 Da
• Isoelectric Point: 5.7980

>NTDB_id=403153 HUW39_RS04560 WP_001542340.1 950634..951614(+) (pilT) [Escherichia coli strain EC31]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRTILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLVNTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=403153 HUW39_RS04560 WP_001542340.1 950634..951614(+) (pilT) [Escherichia coli strain EC31]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGACAATATTGCTGGAGAATGGTCAGTTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCTGCGTTGCGGGAAGATCCTGATGTGATTTTGCTAGGAGAGCTACGTGACAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGACACCTGGTGCTGGCAACTTTACATACACGTGGTGCGGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCAGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCGGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGGTTAACACACCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGACGTTTGTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (62-239)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae strain A1552	49.541	100	0.497
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.541	100	0.497
pilT	Neisseria meningitidis 8013	48.476	100	0.488
pilT	Neisseria gonorrhoeae MS11	48.171	100	0.485
pilT	Acinetobacter baylyi ADP1	46.483	100	0.466
pilT	Acinetobacter baumannii D1279779	46.177	100	0.463
pilT	Acinetobacter nosocomialis M2	46.177	100	0.463
pilT	Acinetobacter baumannii strain A118	46.177	100	0.463
pilT	Pseudomonas stutzeri DSM 10701	46.177	100	0.463
pilT	Pseudomonas aeruginosa PAK	45.872	100	0.46
pilT	Legionella pneumophila strain ERS1305867	45.26	100	0.454
pilT	Legionella pneumophila strain Lp02	45.26	100	0.454
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.138	97.546	0.411
pilU	Vibrio cholerae strain A1552	40.379	97.239	0.393
pilU	Pseudomonas stutzeri DSM 10701	37.273	100	0.377
pilU	Acinetobacter baylyi ADP1	36.646	98.773	0.362
pilB	Legionella pneumophila strain ERS1305867	30.89	100	0.362