Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GJ694_RS19600 Genome accession   NZ_CP046116
Coordinates   4127609..4128589 (-) Length   326 a.a.
NCBI ID   WP_129253131.1    Uniprot ID   -
Organism   Enterobacter cloacae strain CBG15936     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4122609..4133589
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GJ694_RS19570 (GJ694_19570) - 4123485..4123982 (+) 498 WP_003860035.1 SprT family zinc-dependent metalloprotease -
  GJ694_RS19575 (GJ694_19575) endA 4124077..4124784 (+) 708 WP_013098661.1 deoxyribonuclease I -
  GJ694_RS19580 (GJ694_19580) rsmE 4124837..4125568 (+) 732 WP_013098662.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  GJ694_RS19585 (GJ694_19585) gshB 4125588..4126535 (+) 948 WP_023620782.1 glutathione synthase -
  GJ694_RS19590 (GJ694_19590) - 4126622..4127182 (+) 561 WP_129253130.1 YqgE/AlgH family protein -
  GJ694_RS19595 (GJ694_19595) ruvX 4127182..4127598 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  GJ694_RS19600 (GJ694_19600) pilT 4127609..4128589 (-) 981 WP_129253131.1 type IV pilus twitching motility protein PilT Machinery gene
  GJ694_RS19605 (GJ694_19605) - 4128607..4129308 (+) 702 WP_023620785.1 YggS family pyridoxal phosphate-dependent enzyme -
  GJ694_RS19610 (GJ694_19610) - 4129330..4129896 (+) 567 WP_013098667.1 YggT family protein -
  GJ694_RS19615 (GJ694_19615) yggU 4129893..4130180 (+) 288 WP_013098668.1 DUF167 family protein YggU -
  GJ694_RS19620 (GJ694_19620) rdgB 4130193..4130786 (+) 594 WP_129253132.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  GJ694_RS19625 (GJ694_19625) hemW 4130779..4131927 (+) 1149 WP_038983534.1 radical SAM family heme chaperone HemW -
  GJ694_RS19630 (GJ694_19630) - 4131984..4132346 (+) 363 WP_038983532.1 endonuclease domain-containing protein -
  GJ694_RS19635 (GJ694_19635) - 4132414..4133130 (-) 717 WP_023621456.1 DUF2884 domain-containing protein -
  GJ694_RS19640 (GJ694_19640) - 4133187..4133513 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35637.68 Da        Isoelectric Point: 6.3038

>NTDB_id=401196 GJ694_RS19600 WP_129253131.1 4127609..4128589(-) (pilT) [Enterobacter cloacae strain CBG15936]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRVGRLEPAPFPSPDVDALLKTWLNDELQGAWWASGQVDFAVTLTGNQRLRA
SAFKQMKGNSITLRLLPRACPQLSALGVPRAIPELLSHDSGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FVYQSERCLIQQREIGLHSPSFAEALRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQRLLPDLQGGRVALYELLVNTPAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=401196 GJ694_RS19600 WP_129253131.1 4127609..4128589(-) (pilT) [Enterobacter cloacae strain CBG15936]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGAGTAGGCCGCCTTGAACCGGCTCCCTTTCCCTCTCCCGATGTGGATGCGTTACTGAAAACGTGGCTCAACG
ATGAGCTGCAGGGGGCATGGTGGGCAAGCGGGCAGGTGGATTTTGCCGTGACACTTACGGGAAACCAGCGCCTGCGCGCA
AGCGCCTTTAAACAGATGAAGGGCAACTCAATCACGCTACGCCTGTTGCCCCGGGCCTGTCCGCAGCTCTCCGCCCTGGG
GGTTCCCCGGGCTATCCCGGAACTCTTATCCCACGACAGCGGGCTGATACTGGTGACGGGGGCAACCGGCAGCGGCAAAT
CTACCACCCTTGCGGCAATGGTGGATTTTCTTAATCACCAGACGGATGGGCATATTCTGACGCTGGAAGATCCGGTGGAG
TTTGTCTATCAGAGCGAACGTTGCCTGATTCAGCAGCGGGAGATAGGCCTGCACAGTCCCTCATTTGCCGAGGCGCTGCG
CGCTGCGTTGCGTGAAGATCCGGATGTCATCCTGCTGGGAGAACTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CCGCTGAAACCGGGCATCTGGTGCTGGCGACGCTGCACACGCGCGGTGCCTCTCAGGCGATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGACCCGGTGCGTAATCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAAAGACTGCTTCC
CGACCTGCAGGGTGGGCGCGTCGCGCTGTATGAGCTGCTGGTGAATACGCCAGCGGCAGCGAACCTTATCCGCGAAGGTA
AAACGTGGCAGTTGCCCGGGATTATTCAAACCGGTCAGCAGGCGGGGATGCAGAACTTTGACCAGAGCCTGGCAGAGCGA
CGGGCGCAGGGGCGGTTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(64-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Pseudomonas stutzeri DSM 10701

48.318

100

0.485
  pilT Legionella pneumophila strain ERS1305867

48.758

98.773

0.482
  pilT Legionella pneumophila strain Lp02

48.758

98.773

0.482
  pilT Acinetobacter baumannii strain A118

47.706

100

0.479
  pilT Acinetobacter baumannii D1279779

47.706

100

0.479
  pilT Acinetobacter baylyi ADP1

47.401

100

0.475
  pilT Pseudomonas aeruginosa PAK

47.401

100

0.475
  pilT Acinetobacter nosocomialis M2

48.125

98.16

0.472
  pilT Neisseria meningitidis 8013

46.951

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.646

100

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

98.773

0.423
  pilU Pseudomonas stutzeri DSM 10701

38.485

100

0.39
  pilU Vibrio cholerae strain A1552

39.441

98.773

0.39
  pilB Haemophilus influenzae 86-028NP

33.708

100

0.368

Multiple sequence alignment