Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GH975_RS01120 Genome accession   NZ_CP045871
Coordinates   219285..220322 (+) Length   345 a.a.
NCBI ID   WP_153712738.1    Uniprot ID   A0A5Q2Q8R2
Organism   Litorivicinus lipolyticus strain IMCC 1097     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 214285..225322
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GH975_RS01085 (GH975_01085) rdgB 214453..215037 (-) 585 WP_153712731.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  GH975_RS01090 (GH975_01090) - 215034..215465 (-) 432 WP_153712732.1 DUF4426 domain-containing protein -
  GH975_RS01095 (GH975_01095) metW 215462..216061 (-) 600 WP_153712733.1 methionine biosynthesis protein MetW -
  GH975_RS01100 (GH975_01100) - 216058..217152 (-) 1095 WP_153712734.1 homoserine O-acetyltransferase -
  GH975_RS01105 (GH975_01105) - 217153..217734 (-) 582 WP_153712735.1 YggT family protein -
  GH975_RS01110 (GH975_01110) proC 217758..218558 (-) 801 WP_153712736.1 pyrroline-5-carboxylate reductase -
  GH975_RS01115 (GH975_01115) - 218555..219253 (-) 699 WP_153712737.1 YggS family pyridoxal phosphate-dependent enzyme -
  GH975_RS01120 (GH975_01120) pilT 219285..220322 (+) 1038 WP_153712738.1 type IV pilus twitching motility protein PilT Machinery gene
  GH975_RS01125 (GH975_01125) pilU 220332..221450 (+) 1119 WP_153712739.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GH975_RS01130 (GH975_01130) - 221523..222362 (+) 840 WP_153712740.1 MBL fold metallo-hydrolase -
  GH975_RS01135 (GH975_01135) - 222327..223208 (-) 882 WP_153712741.1 hypothetical protein -
  GH975_RS01140 (GH975_01140) - 223205..224173 (-) 969 WP_153712742.1 aspartate carbamoyltransferase catalytic subunit -
  GH975_RS01145 (GH975_01145) ruvX 224217..224630 (-) 414 WP_153712743.1 Holliday junction resolvase RuvX -
  GH975_RS01150 (GH975_01150) - 224627..225181 (-) 555 WP_153712744.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 37916.37 Da        Isoelectric Point: 6.5087

>NTDB_id=398911 GH975_RS01120 WP_153712738.1 219285..220322(+) (pilT) [Litorivicinus lipolyticus strain IMCC 1097]
MDITELLAFSAKQKASDLHLSAGLPPMIRVDGDVRRINVPELDHKTVHGLIYDIMNDKQRRDFEEKLETDFSFEVPGVAR
FRVNAFNQNRGAAAVFRTIPTEILSMEALGMGQVFRNLSDKPRGLVLVTGPTGSGKSTTLAAMVDYINETRNDHILTIED
PIEFVHPSKKSLVNQREVHRDTHGFSDALRSALREDPDVILVGEMRDLETISLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPAAEKDMVRSMLSESLQGVISQTLLKKNGGGRVAAHEIMMGTPAIRNLIREDKVAQMVSAIQTGSSMGMVTLDQSL
QTLVDKGVVSKEVARTKASRPDTFN

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=398911 GH975_RS01120 WP_153712738.1 219285..220322(+) (pilT) [Litorivicinus lipolyticus strain IMCC 1097]
ATGGATATCACAGAGTTATTGGCCTTTAGCGCCAAACAGAAGGCCTCGGATTTACACCTGTCGGCGGGTTTGCCGCCGAT
GATTCGGGTGGACGGTGATGTCCGGCGTATCAATGTGCCTGAACTTGACCATAAAACCGTGCATGGGCTGATTTACGACA
TCATGAACGACAAGCAACGACGTGACTTTGAAGAGAAGCTGGAAACAGACTTTTCGTTTGAAGTGCCCGGTGTGGCGCGT
TTTCGTGTCAACGCCTTTAATCAAAACCGTGGCGCGGCGGCGGTGTTCCGGACCATTCCAACCGAAATTTTGAGTATGGA
AGCGCTCGGCATGGGGCAGGTGTTCCGCAACTTGTCGGACAAACCGCGGGGTTTGGTGCTGGTGACCGGCCCGACCGGTT
CGGGCAAGTCGACCACGCTGGCGGCCATGGTGGACTACATCAACGAGACCCGAAACGATCACATCTTGACGATCGAAGAT
CCGATCGAGTTTGTGCACCCGTCGAAAAAATCCCTGGTCAACCAGCGTGAAGTCCACCGCGACACCCATGGGTTTAGCGA
CGCCCTGCGCAGCGCCCTGCGCGAAGACCCGGACGTGATTTTGGTCGGCGAGATGCGTGATTTAGAAACCATTAGCTTGG
CGCTGACGGCGGCTGAAACTGGCCACCTGGTGTTCGGCACGCTGCACACTACCAGTGCCGCCAAAACGATCGACCGTATT
ATCGACGTCTTCCCGGCGGCCGAGAAAGACATGGTCCGCTCGATGCTGTCCGAATCCTTGCAAGGCGTGATCAGCCAAAC
GCTATTGAAAAAGAATGGCGGCGGCCGGGTCGCGGCCCATGAAATCATGATGGGTACACCGGCGATTCGTAACCTGATAC
GCGAGGACAAGGTCGCGCAAATGGTCAGTGCGATCCAAACCGGCAGCTCGATGGGCATGGTTACCTTGGATCAGTCCCTA
CAGACCCTGGTCGATAAGGGGGTGGTCTCTAAAGAGGTTGCCCGGACCAAGGCATCCCGCCCCGATACGTTTAATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5Q2Q8R2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

83.43

99.71

0.832
  pilT Pseudomonas stutzeri DSM 10701

82.267

99.71

0.82
  pilT Acinetobacter baumannii strain A118

81.686

99.71

0.814
  pilT Acinetobacter nosocomialis M2

81.686

99.71

0.814
  pilT Acinetobacter baumannii D1279779

81.686

99.71

0.814
  pilT Acinetobacter baylyi ADP1

78.779

99.71

0.786
  pilT Legionella pneumophila strain Lp02

72.674

99.71

0.725
  pilT Legionella pneumophila strain ERS1305867

72.674

99.71

0.725
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.03

97.681

0.684
  pilT Vibrio cholerae strain A1552

70.03

97.681

0.684
  pilT Neisseria meningitidis 8013

67.052

100

0.672
  pilT Neisseria gonorrhoeae MS11

66.474

100

0.667
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.519
  pilU Pseudomonas stutzeri DSM 10701

41.593

98.261

0.409
  pilU Vibrio cholerae strain A1552

41.791

97.101

0.406
  pilU Acinetobacter baylyi ADP1

39.017

100

0.391

Multiple sequence alignment