Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACAV_RS02955 Genome accession   NC_015138
Coordinates   645679..646722 (-) Length   347 a.a.
NCBI ID   WP_011793812.1    Uniprot ID   A1TJV3
Organism   Paracidovorax avenae ATCC 19860     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 640679..651722
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAV_RS02925 (Acav_0601) - 640933..641340 (+) 408 WP_041828534.1 YraN family protein -
  ACAV_RS02930 (Acav_0602) - 641412..642014 (+) 603 WP_013593084.1 SIS domain-containing protein -
  ACAV_RS02935 (Acav_0603) - 642011..642667 (+) 657 WP_013593085.1 BON domain-containing protein -
  ACAV_RS02940 (Acav_0604) - 642767..643705 (-) 939 WP_013593086.1 NAD(P)-dependent oxidoreductase -
  ACAV_RS02945 (Acav_0605) pilU 643789..644925 (-) 1137 WP_013593087.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACAV_RS02950 (Acav_0606) - 644953..645591 (-) 639 WP_013593088.1 cyclic nucleotide-binding domain-containing protein -
  ACAV_RS02955 (Acav_0607) pilT 645679..646722 (-) 1044 WP_011793812.1 type IV pilus twitching motility protein PilT Machinery gene
  ACAV_RS02960 (Acav_0608) - 646764..647486 (+) 723 WP_013593089.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACAV_RS02965 (Acav_0609) ltaE 647498..648562 (-) 1065 WP_013593090.1 low-specificity L-threonine aldolase -
  ACAV_RS02970 (Acav_0610) - 648767..649906 (+) 1140 WP_013593091.1 aminotransferase class V-fold PLP-dependent enzyme -
  ACAV_RS02975 (Acav_0611) - 650118..651542 (+) 1425 WP_013593092.1 DUF445 domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38132.93 Da        Isoelectric Point: 6.8354

>NTDB_id=39866 ACAV_RS02955 WP_011793812.1 645679..646722(-) (pilT) [Paracidovorax avenae ATCC 19860]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDALDHKTVHAMVYDIMSDAQRKVYEEFLEVDFSFEIEGLAR
FRVNAFNQNRGAAAVFRTIPSKILTLEQLNAPRIFADLALKPRGLVLVTGPTGSGKSTTLAAMVNHLNETEYGHILTVED
PIEFVHDSKKCLINQREVGPMTLSFAAALKSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTSAIRNLIREAKVAQMYSTIQTSSSVGMQTLDQN
LTDLVRRNIISPAEARGKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=39866 ACAV_RS02955 WP_011793812.1 645679..646722(-) (pilT) [Paracidovorax avenae ATCC 19860]
GTGGACATCACCCAACTGCTCGCCTTCAGCGTGAAGAACAAGGCTTCCGACCTGCACCTGTCCGCGGGCCTGCCTCCGAT
GATCCGCGTGCATGGCGACGTGCGCCGCATCAACGTCGATGCGCTCGACCACAAGACGGTGCATGCCATGGTGTACGACA
TCATGAGCGATGCCCAGCGCAAGGTGTACGAGGAGTTCCTGGAAGTGGACTTCTCGTTCGAGATCGAGGGCCTGGCGCGC
TTCCGGGTCAACGCCTTCAACCAGAACCGCGGCGCGGCCGCCGTGTTCCGGACCATCCCGAGCAAGATCCTCACGCTCGA
GCAACTCAATGCCCCGCGCATCTTCGCCGACCTCGCCCTCAAGCCGCGCGGCCTCGTGCTCGTCACCGGCCCCACGGGCT
CGGGCAAGTCCACCACGCTCGCGGCCATGGTGAACCACCTCAACGAGACCGAGTACGGGCACATCCTCACGGTGGAAGAC
CCGATCGAGTTCGTGCACGACTCCAAGAAGTGCCTGATCAACCAGCGCGAGGTCGGGCCGATGACGCTGTCCTTCGCCGC
CGCGCTGAAATCCGCGCTGCGCGAAGACCCGGACGCCATCCTCGTGGGTGAAATGCGCGACCTCGAGACCATCCGCCTGG
CCATGACGGCCGCCGAGACGGGCCACCTCGTGTTCGGCACCCTGCACACCTCCAGCGCCGCCAAGACCATCGACCGGATC
ATCGACGTGTTCCCCGCCGAGGAAAAGGAGATGGTGCGCGCCATGCTGTCCGAATCGCTGCAGGCCGTGATCTCCCAGAC
GCTGTGCAAGACCAAGGACGGCCAGGGCCGCGTGGCGGCGCACGAGATCATGCTGGGCACCAGCGCCATCCGCAACCTGA
TCCGCGAGGCCAAGGTGGCGCAGATGTACTCCACCATCCAGACCAGCAGCAGCGTCGGCATGCAGACGCTGGACCAGAAC
CTCACCGACCTCGTGCGGCGCAACATCATCAGCCCCGCGGAAGCCCGCGGCAAGGCCAAGATTCCGGAGAACTTCCCGGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A1TJV3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

76.232

99.424

0.758
  pilT Acinetobacter nosocomialis M2

76.232

99.424

0.758
  pilT Acinetobacter baumannii D1279779

76.232

99.424

0.758
  pilT Acinetobacter baumannii strain A118

76.232

99.424

0.758
  pilT Pseudomonas stutzeri DSM 10701

75.652

99.424

0.752
  pilT Acinetobacter baylyi ADP1

74.493

99.424

0.741
  pilT Legionella pneumophila strain ERS1305867

73.451

97.695

0.718
  pilT Legionella pneumophila strain Lp02

73.451

97.695

0.718
  pilT Neisseria gonorrhoeae MS11

67.826

99.424

0.674
  pilT Neisseria meningitidis 8013

67.826

99.424

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.155

98.271

0.66
  pilT Vibrio cholerae strain A1552

67.155

98.271

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

99.424

0.504
  pilU Vibrio cholerae strain A1552

43.881

96.542

0.424
  pilU Acinetobacter baylyi ADP1

41.618

99.712

0.415
  pilU Pseudomonas stutzeri DSM 10701

41.493

96.542

0.401

Multiple sequence alignment