Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACAV_RS02945 Genome accession   NC_015138
Coordinates   643789..644925 (-) Length   378 a.a.
NCBI ID   WP_013593087.1    Uniprot ID   F0Q6A0
Organism   Paracidovorax avenae ATCC 19860     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 638789..649925
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAV_RS02920 (Acav_0600) rsmI 639974..640933 (-) 960 WP_013593082.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  ACAV_RS02925 (Acav_0601) - 640933..641340 (+) 408 WP_041828534.1 YraN family protein -
  ACAV_RS02930 (Acav_0602) - 641412..642014 (+) 603 WP_013593084.1 SIS domain-containing protein -
  ACAV_RS02935 (Acav_0603) - 642011..642667 (+) 657 WP_013593085.1 BON domain-containing protein -
  ACAV_RS02940 (Acav_0604) - 642767..643705 (-) 939 WP_013593086.1 NAD(P)-dependent oxidoreductase -
  ACAV_RS02945 (Acav_0605) pilU 643789..644925 (-) 1137 WP_013593087.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACAV_RS02950 (Acav_0606) - 644953..645591 (-) 639 WP_013593088.1 cyclic nucleotide-binding domain-containing protein -
  ACAV_RS02955 (Acav_0607) pilT 645679..646722 (-) 1044 WP_011793812.1 type IV pilus twitching motility protein PilT Machinery gene
  ACAV_RS02960 (Acav_0608) - 646764..647486 (+) 723 WP_013593089.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACAV_RS02965 (Acav_0609) ltaE 647498..648562 (-) 1065 WP_013593090.1 low-specificity L-threonine aldolase -
  ACAV_RS02970 (Acav_0610) - 648767..649906 (+) 1140 WP_013593091.1 aminotransferase class V-fold PLP-dependent enzyme -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41718.14 Da        Isoelectric Point: 6.9079

>NTDB_id=39865 ACAV_RS02945 WP_013593087.1 643789..644925(-) (pilU) [Paracidovorax avenae ATCC 19860]
MERDQASKFINDLLKLMVGRGGSDLFITAEFPPAIKVDGKVTKVSPQPLTPAHTLTLARAIMSDKQVADFERTKECNFAI
SPAGIGRFRVNAFIQQGRVGMVLRTIPLTLPTIDGLGVPQVLKEITMSKRGLCILVGATGSGKSTTLAAMVDWRNENSFG
HIITVEDPIEFVHPHKNCVVTQREVGLDTDSWEAALKNTLRQAPDVILMGEIRDRETMDHAIAFSETGHLCLATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRAMVSQRLIPKQDGKGRAAAVEVMLNTPLIADLIFKGEVAEIKEIMKKSRNLG
MQTFDQSLFDLFEANVIGYEDALRNADSLNDLRLQIKLGSQRARSTDLSSGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=39865 ACAV_RS02945 WP_013593087.1 643789..644925(-) (pilU) [Paracidovorax avenae ATCC 19860]
ATGGAACGCGATCAGGCCAGCAAATTCATCAACGACCTGCTGAAGCTCATGGTGGGGCGCGGCGGCAGCGACCTTTTCAT
CACGGCGGAATTCCCCCCTGCCATCAAGGTGGACGGGAAGGTCACGAAGGTCTCCCCCCAGCCGCTCACGCCGGCGCACA
CCCTCACCCTTGCGCGCGCCATCATGAGCGACAAGCAGGTGGCGGATTTCGAGCGCACGAAGGAATGCAACTTCGCCATA
TCCCCCGCGGGCATCGGCCGCTTCCGCGTGAACGCCTTCATCCAGCAGGGCCGCGTGGGCATGGTGCTGCGGACCATCCC
GCTCACGCTGCCCACCATCGACGGGCTGGGCGTGCCGCAGGTGCTCAAGGAAATCACCATGTCCAAGCGCGGGCTGTGCA
TCCTCGTGGGTGCCACCGGCTCGGGCAAGTCCACCACCCTCGCGGCCATGGTGGACTGGCGCAACGAGAACTCCTTCGGC
CACATCATCACGGTGGAAGACCCCATCGAGTTCGTGCACCCGCACAAGAACTGCGTGGTCACCCAGCGCGAGGTGGGCCT
GGACACCGACAGCTGGGAGGCGGCCCTCAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTCATGGGAGAAATCCGCG
ACCGCGAGACCATGGACCATGCCATCGCCTTCTCCGAGACCGGCCACCTGTGCCTCGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGGATCATCAACTTCTTCCCCGAGGAACGGCGCGCGCAGCTGCTCATGGACCTGTCGCTCAACCT
GCGCGCCATGGTGTCCCAGCGGCTCATCCCCAAGCAGGACGGCAAGGGCCGCGCCGCGGCGGTGGAAGTCATGCTCAACA
CGCCCCTGATCGCCGACCTGATCTTCAAGGGTGAAGTCGCCGAGATCAAGGAGATCATGAAGAAGAGCCGCAACCTCGGC
ATGCAGACCTTCGACCAGTCGCTGTTCGACCTCTTCGAGGCCAACGTCATCGGCTACGAGGACGCCCTGCGCAACGCCGA
CTCGCTCAACGACCTGCGGCTGCAGATCAAGCTCGGCAGCCAGCGCGCCCGCAGCACCGACCTGTCGTCCGGCACCGAGC
ACTTCGCCATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F0Q6A0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.47

92.328

0.595
  pilU Acinetobacter baylyi ADP1

57.542

94.709

0.545
  pilU Vibrio cholerae strain A1552

52.617

96.032

0.505
  pilT Pseudomonas aeruginosa PAK

44.807

89.153

0.399
  pilT Acinetobacter baumannii strain A118

45.045

88.095

0.397
  pilT Acinetobacter nosocomialis M2

45.045

88.095

0.397
  pilT Acinetobacter baumannii D1279779

45.045

88.095

0.397
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.214

89.153

0.394
  pilT Legionella pneumophila strain Lp02

44.144

88.095

0.389
  pilT Legionella pneumophila strain ERS1305867

44.144

88.095

0.389
  pilT Pseudomonas stutzeri DSM 10701

43.62

89.153

0.389
  pilT Acinetobacter baylyi ADP1

43.243

88.095

0.381
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.169

87.831

0.37
  pilT Vibrio cholerae strain A1552

42.169

87.831

0.37

Multiple sequence alignment