Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GCD22_RS01730 Genome accession   NZ_CP045571
Coordinates   330596..331720 (+) Length   374 a.a.
NCBI ID   WP_024893379.1    Uniprot ID   A0A5P9XNF2
Organism   Acidithiobacillus thiooxidans ATCC 19377     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 325596..336720
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GCD22_RS01700 (GCD22_00348) - 326051..326614 (+) 564 WP_024893374.1 acyloxyacyl hydrolase -
  GCD22_RS01705 (GCD22_00349) bioB 326674..327618 (-) 945 WP_031569515.1 biotin synthase BioB -
  GCD22_RS01710 - 327656..327937 (-) 282 WP_031569513.1 YggT family protein -
  GCD22_RS01715 (GCD22_00351) proC 327934..328761 (-) 828 WP_031569512.1 pyrroline-5-carboxylate reductase -
  GCD22_RS01720 - 328758..329429 (-) 672 WP_031569511.1 YggS family pyridoxal phosphate-dependent enzyme -
  GCD22_RS01725 (GCD22_00353) pilT 329536..330585 (+) 1050 WP_153940361.1 type IV pilus twitching motility protein PilT Machinery gene
  GCD22_RS01730 (GCD22_00354) pilU 330596..331720 (+) 1125 WP_024893379.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GCD22_RS01735 (GCD22_00355) - 331732..332922 (+) 1191 WP_031569509.1 glycosyltransferase -
  GCD22_RS01740 (GCD22_00356) - 332948..334075 (-) 1128 WP_031569507.1 FAD-dependent oxidoreductase -
  GCD22_RS01745 (GCD22_00358) trmB 334396..335088 (-) 693 WP_080707745.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
  GCD22_RS01750 (GCD22_00359) purE 335130..335627 (+) 498 WP_031569989.1 5-(carboxyamino)imidazole ribonucleotide mutase -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 41493.91 Da        Isoelectric Point: 6.4007

>NTDB_id=395255 GCD22_RS01730 WP_024893379.1 330596..331720(+) (pilU) [Acidithiobacillus thiooxidans ATCC 19377]
MSVLDDLLALMVEKNGSDLYLTVGSPPVMKIDGRAVNLGTDMLKPGQVLSLAKEILGMERLQEFQHEKEINMAISATGIG
RFRVNGFYQRGELSFVLRAIKTQIPTLEQLKLPPILKDLAMTSRGLVLFVGATGSGKSTSLASMIQYRNQNAAGHILTIE
DPIEFIHKNQKSIVNQREVGIDTLSYERALENALREAPDVILIGEVRNRDTMDHAMAYAETGHLCMSTLHANNANQAIER
IINFFPEDRKKQLLMDLSLNLKAVVSQRLLPIKGQAGRIAAIEVLINTPAIADLIYKGEVGMLKEAMGRTNDVGMQTFDR
SLLNLYAQGLVEYEDALRAADSQNDLRLAIKQDCLRRGLDDPGVKHSSEDKWQI

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=395255 GCD22_RS01730 WP_024893379.1 330596..331720(+) (pilU) [Acidithiobacillus thiooxidans ATCC 19377]
ATGTCTGTACTGGATGATCTGCTGGCATTGATGGTCGAAAAAAACGGTTCTGATTTGTATCTGACTGTGGGTTCTCCGCC
GGTCATGAAAATTGATGGACGCGCCGTAAATCTGGGTACAGATATGTTGAAACCCGGGCAAGTGCTCAGTCTCGCCAAGG
AAATTCTGGGGATGGAGCGTCTGCAGGAATTTCAGCACGAAAAAGAAATCAACATGGCTATTTCAGCTACAGGCATCGGG
CGTTTTCGGGTCAATGGATTTTATCAGCGTGGTGAACTGAGTTTTGTGTTGCGTGCCATTAAAACCCAAATCCCGACCCT
GGAGCAGCTCAAATTGCCACCCATTCTGAAGGATCTCGCCATGACTTCGCGAGGTCTGGTGCTGTTTGTGGGCGCGACCG
GTTCGGGTAAAAGTACCTCATTGGCTTCCATGATTCAGTATCGTAATCAGAATGCTGCCGGTCATATCCTGACCATCGAA
GATCCTATTGAATTTATTCATAAGAATCAGAAATCGATTGTCAATCAGCGGGAAGTCGGCATAGATACGCTTAGTTATGA
AAGAGCACTGGAAAATGCCCTGCGTGAGGCGCCTGATGTGATTTTGATTGGTGAGGTGCGTAATAGGGATACAATGGATC
ACGCCATGGCCTACGCAGAAACCGGGCACTTATGCATGTCTACCCTGCATGCCAATAATGCCAACCAGGCCATTGAACGC
ATCATCAACTTTTTCCCCGAAGATCGGAAAAAGCAGTTGCTGATGGATTTATCCCTGAATCTGAAAGCCGTTGTTTCCCA
GCGACTTCTGCCCATCAAGGGGCAGGCGGGACGTATTGCCGCCATTGAGGTGTTGATTAATACGCCGGCGATTGCTGATC
TTATCTACAAAGGCGAAGTCGGAATGCTCAAGGAAGCCATGGGTCGCACCAATGATGTGGGCATGCAAACCTTTGATCGG
AGTCTGCTGAATTTATACGCTCAGGGTTTGGTGGAATATGAAGATGCGCTCCGTGCCGCCGATTCTCAAAATGATTTACG
TTTGGCTATCAAGCAGGATTGCTTGCGTCGCGGTTTGGATGACCCAGGGGTTAAGCATTCTTCCGAGGATAAGTGGCAAA
TATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-250)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5P9XNF2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.931

93.048

0.586
  pilU Vibrio cholerae strain A1552

52.778

96.257

0.508
  pilU Acinetobacter baylyi ADP1

54.441

93.316

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.939

92.781

0.398
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.939

88.235

0.388
  pilT Vibrio cholerae strain A1552

43.939

88.235

0.388
  pilT Legionella pneumophila strain ERS1305867

42.73

90.107

0.385
  pilT Legionella pneumophila strain Lp02

42.73

90.107

0.385
  pilT Acinetobacter baylyi ADP1

41.298

90.642

0.374
  pilT Pseudomonas aeruginosa PAK

41.246

90.107

0.372
  pilT Acinetobacter baumannii D1279779

40.95

90.107

0.369
  pilT Acinetobacter baumannii strain A118

40.95

90.107

0.369
  pilT Acinetobacter nosocomialis M2

40.653

90.107

0.366
  pilT Pseudomonas stutzeri DSM 10701

40.653

90.107

0.366
  pilT Neisseria meningitidis 8013

39.306

92.513

0.364

Multiple sequence alignment