Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GCD22_RS01725 Genome accession   NZ_CP045571
Coordinates   329536..330585 (+) Length   349 a.a.
NCBI ID   WP_153940361.1    Uniprot ID   A0A5P9XLQ3
Organism   Acidithiobacillus thiooxidans ATCC 19377     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 324536..335585
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GCD22_RS01695 (GCD22_00347) - 324795..325808 (-) 1014 WP_010641319.1 HlyD family secretion protein -
  GCD22_RS01700 (GCD22_00348) - 326051..326614 (+) 564 WP_024893374.1 acyloxyacyl hydrolase -
  GCD22_RS01705 (GCD22_00349) bioB 326674..327618 (-) 945 WP_031569515.1 biotin synthase BioB -
  GCD22_RS01710 - 327656..327937 (-) 282 WP_031569513.1 YggT family protein -
  GCD22_RS01715 (GCD22_00351) proC 327934..328761 (-) 828 WP_031569512.1 pyrroline-5-carboxylate reductase -
  GCD22_RS01720 - 328758..329429 (-) 672 WP_031569511.1 YggS family pyridoxal phosphate-dependent enzyme -
  GCD22_RS01725 (GCD22_00353) pilT 329536..330585 (+) 1050 WP_153940361.1 type IV pilus twitching motility protein PilT Machinery gene
  GCD22_RS01730 (GCD22_00354) pilU 330596..331720 (+) 1125 WP_024893379.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GCD22_RS01735 (GCD22_00355) - 331732..332922 (+) 1191 WP_031569509.1 glycosyltransferase -
  GCD22_RS01740 (GCD22_00356) - 332948..334075 (-) 1128 WP_031569507.1 FAD-dependent oxidoreductase -
  GCD22_RS01745 (GCD22_00358) trmB 334396..335088 (-) 693 WP_080707745.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -

Sequence

Protein


Download         Length: 349 a.a.        Molecular weight: 38550.19 Da        Isoelectric Point: 6.4320

>NTDB_id=395254 GCD22_RS01725 WP_153940361.1 329536..330585(+) (pilT) [Acidithiobacillus thiooxidans ATCC 19377]
MDIAELLSFAVKNNASDTHISAGLAPMLRINGDIRPINVEALDGKAVHAMIYDIMSDSQRKWYEENLEIDFAYELPGIAR
FRVNAFNQDRGPAAAFHTIPAKVLSLEDLNAPKSFTEIINIPRGLILVTGPTGSGKSTTLAAMVDHINANRPDHIITIED
PIEFIHTAKKCLINQREVGPNTHSFENALRSALREDPDIILVGELRDLETMRLALTAAETGHIVFATLHTSSAPKTIDRI
VDSFPGGEKDMVRSMLSESLRAVISQTLLKTADGKGRVPAHEIMIATPAIRNLIRENKVAQMYSVIQTGQNQGMQTLDQC
LADLVRAHKITREEAMRRVQNKDSFMNVA

Nucleotide


Download         Length: 1050 bp        

>NTDB_id=395254 GCD22_RS01725 WP_153940361.1 329536..330585(+) (pilT) [Acidithiobacillus thiooxidans ATCC 19377]
ATGGATATTGCTGAACTACTCAGCTTCGCGGTGAAAAATAACGCATCAGATACGCACATATCTGCGGGCCTCGCACCCAT
GTTGCGCATTAACGGCGATATACGTCCCATCAACGTTGAAGCACTGGACGGAAAGGCCGTCCACGCAATGATTTACGACA
TCATGTCGGATTCCCAGCGAAAATGGTATGAAGAAAATCTGGAAATCGATTTTGCCTATGAACTGCCCGGTATTGCGCGT
TTTCGGGTGAATGCTTTTAATCAGGATCGGGGTCCCGCTGCGGCATTTCATACCATTCCGGCCAAGGTGTTGAGTCTGGA
AGATCTCAATGCGCCAAAATCTTTTACGGAAATTATTAATATCCCGCGTGGACTGATACTGGTTACCGGACCAACCGGGT
CTGGTAAGTCCACGACCCTGGCGGCTATGGTGGATCATATCAACGCTAATCGGCCAGATCATATTATTACCATCGAAGAT
CCTATAGAGTTCATCCATACGGCTAAAAAGTGCCTGATTAATCAGCGTGAAGTGGGGCCGAATACCCATTCCTTCGAAAA
CGCCCTGCGTTCGGCGCTGCGTGAAGATCCGGATATTATTCTGGTCGGTGAGTTGCGTGATCTGGAAACCATGCGGCTTG
CCTTGACGGCAGCAGAAACCGGACACATTGTTTTTGCGACGCTACATACCAGTTCAGCACCTAAAACCATTGATCGTATT
GTCGATTCTTTCCCGGGCGGGGAAAAGGACATGGTCCGCTCCATGTTGTCTGAATCCTTACGGGCGGTCATTTCGCAAAC
CCTGCTGAAAACTGCCGATGGAAAAGGGCGGGTTCCAGCTCATGAAATCATGATTGCCACGCCGGCTATTCGTAATCTCA
TTCGCGAAAATAAAGTGGCACAAATGTACTCAGTCATTCAGACCGGACAAAACCAGGGCATGCAAACGCTGGATCAATGC
CTGGCAGATTTGGTGCGTGCCCATAAAATTACTCGCGAAGAGGCAATGCGCCGGGTGCAGAACAAAGACAGTTTTATGAA
TGTGGCCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5P9XLQ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

67.63

99.14

0.67
  pilT Acinetobacter baumannii strain A118

67.63

99.14

0.67
  pilT Acinetobacter nosocomialis M2

67.63

99.14

0.67
  pilT Pseudomonas stutzeri DSM 10701

67.246

98.854

0.665
  pilT Pseudomonas aeruginosa PAK

66.667

98.854

0.659
  pilT Acinetobacter baylyi ADP1

66.377

98.854

0.656
  pilT Legionella pneumophila strain Lp02

65.507

98.854

0.648
  pilT Legionella pneumophila strain ERS1305867

65.507

98.854

0.648
  pilT Neisseria meningitidis 8013

64.928

98.854

0.642
  pilT Neisseria gonorrhoeae MS11

64.638

98.854

0.639
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65

97.421

0.633
  pilT Vibrio cholerae strain A1552

65

97.421

0.633
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.284

100

0.493
  pilU Acinetobacter baylyi ADP1

42.899

96.848

0.415
  pilU Pseudomonas stutzeri DSM 10701

41.369

96.275

0.398
  pilU Vibrio cholerae strain A1552

39.286

96.275

0.378

Multiple sequence alignment